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ATP1A1_G4_Q118R_Std_BFP-to-EGFP_Dual_epegRNA_tevopreQ1

Plasmid

#187459

Purpose

Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 pegRNA and EBFP_To_EGFP epegRNA (tevopreq1) from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G4 Q118R pegRNA + EBFP to EGFP epegRNA (tevopreq1) (ATP1A1 Human)

Use

CRISPR; Prime editing

Tags

Expression

Mammalian

Mutation

Promoter

Tandem U6 promoters

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GFP(9-7)

Plasmid

#165145

Purpose

Expresses GFP(9-7) in mammalian cells

Depositor

Daichi Kamiyama

Article

Tamura et al Commun Biol. 2021 Feb 26;4(1):257. doi:

Insert

GFP(9-7)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hFGD5

Plasmid

#50760

Purpose

expression of EGFP-tagged human FGD5

Depositor

Naoki Mochizuki

Article

Ando et al J Cell Biol. 2013 Sep 16;202(6):901-16.

Insert

FGD5 (FGD5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-PH1(WT)

Plasmid

#86599

Purpose

Expresses eGFP-tagged PH(WT) domain of human Akt1

Depositor

Thomas Leonard, Ivan Yudushkin

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 PH domain (AKT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pGFP-mHP1alpha

Plasmid

#181900

Purpose

Fluorescently tagged HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-rSyntenin1

Plasmid

#89431

Purpose

mammalian expressio of EGFP tagged rat Syntenin1

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-(SIM)3

Plasmid

#126955

Purpose

Bacterial expression plasmid for eGFP-(SIM)3 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)

Use

Tags

eGFP

Expression

Bacterial

Mutation

Promoter

unknown

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pGFP-mHP1beta

Plasmid

#181901

Purpose

Fluorescently tagged HP1beta

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1beta (Cbx1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pKTol2C-EGFP

Plasmid

#85598

Purpose

Tol2 transposon with EGFP driven by minimal-CAGs promoter.

Depositor

Karl Clark, Scott Fahrenkrug

Article

Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

Insert

Tol2 transposon with EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

mini-CAG

Available since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-Sec3

Plasmid

#53755

Purpose

pEGFP-C3 plasmid that expresses Sec3

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Sec3 (EXOC1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
FlucSM-HA-GFP11-N1

Plasmid

#91955

Purpose

Expresses aggregation-prone FlucSM-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

FlucSM-HA-GFP11

Use

Tags

Expression

Mammalian

Mutation

R188Q

Promoter

CMV

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-PRKAA1

Plasmid

#30305

Depositor

Jay Brenman

Article

Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

Insert

AMP-activated Protein Kinase α1 (Prkaa1 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2011

Availability

Academic Institutions and Nonprofits only

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pTXB1-sfGFP

Plasmid

#51562

Purpose

Expression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)

Depositor

Sankar Adhya, Francisco Malagon

Insert

superfolder GFP

Use

Tags

Intein-Chiting Binding Domanin

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pCEFLmGFP-Gli2

Plasmid

#37672

Depositor

Philip Beachy

Article

Kim et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

Insert

Gli2 (Gli2 Mouse)

Use

Tags

mGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

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pLVX Nup62Fv2GFP

Plasmid

#139683

Purpose

Nup62 dimerization construct fused to 2 copies of GFP under an CMV promoter

Depositor

Edward Campbell

Article

Dharan et al Nat Microbiol. 2020 Jun 1. pii: 10.1038/

Insert

Nup62 (NUP62 Human)

Use

Lentiviral

Tags

2 copies of GFP and Dimerization domain FKBP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-H2B-6

Plasmid

#56436

Purpose

Localization: Nucleus/Histones, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

Dec. 23, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-H2B_Halo_T2A_EGFP

Plasmid

#135444

Purpose

Mamalian cell expression of nuclear HaloTag7 and cytosolic EGFP

Depositor

Kai Johnsson

Article

Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

Insert

H2B-HaloTag7 and EGFP

Use

Tags

H2B

Expression

Mammalian

Mutation

Promoter

CMV/TetO

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec24D

Plasmid

#32678

Depositor

Henry Lester

Article

Richards et al J Biol Chem. 2011 Sep 9;286(36):31241-9.

Insert

Sec24D (SEC24D Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 13, 2012

Availability

Academic Institutions and Nonprofits only

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EGFP-m-Paxillin

Plasmid

#80023

Purpose

Mammalian expression of mouse EGFP-Paxillin

Depositor

Chinten James Lim

Insert

Paxillin (Pxn Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pGFPns-reporter

Plasmid

#35626

Depositor

Howard Gu

Article

Naughton et al Anal Biochem. 2011 Oct 1;417(1):162-4. E

Type

Empty backbone

Use

Reporter

Tags

GFPns

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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