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AAV:ITR-U6-sgRNA(backbone)-pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR

Plasmid

#60226

Purpose

Expresses Cre recombinase and Renilla luciferase from the EFS promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Renilla luciferase
Cre recombinase

Use

AAV, CRISPR, Cre/Lox, Luciferase, and Mouse Targe…

Tags

Cre-HA and Rluc-P2A

Expression

Mammalian

Promoter

EFS and U6

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro

Plasmid

#141370

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp5 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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EGFP-IC2-FL

Plasmid

#51409

Purpose

expression of EGFP-tagged full-length dynein intermediate chain IC2C in mammalian cells

Depositor

Trina Schroer

Article

King et al Mol Biol Cell. 2003 Dec;14(12):5089-97.

Insert

dynein intermediate chain IC2C (Dync1i2 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-C1

Plasmid

#60560

Purpose

Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-hSOX2

Plasmid

#17218

Purpose

Retroviral expression of human SOX2

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2007 Nov 30. 131(5):861-72.

Insert

SOX2 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-pegRNA-RNF2_+5GtoT

Plasmid

#135957

Purpose

S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_5GtoT pegRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-MetGFP

Plasmid

#37560

Depositor

David Rimm

Insert

MET (MET Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP VAMP3

Plasmid

#42310

Depositor

Thierry Galli

Article

Galli et al Mol Biol Cell. 1998 Jun;9(6):1437-48.

Insert

VAMP 3 (VAMP3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pQLinkN

Plasmid

#13670

Depositor

Konrad Buessow

Article

Scheich et al Nucleic Acids Res. 2007;35(6):e43. doi:

Insert

multi cloning site

Use

Co-expression

Expression

Bacterial

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only

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pCMVTNT PINK1 C-myc

Plasmid

#13314

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Tags

myc

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pBABE-zeo largeTcDNA

Plasmid

#1779

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

large T antigen (SV40gp6 simian virus)

Use

Retroviral

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3-SARS-CoV-2-S-RBD-sfGFP

Plasmid

#141184

Purpose

Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Has Service

Cloning Grade DNA

Insert

S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)

Tags

Signal peptide from influenza HA and Superfolder …

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

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xCas9 3.7

Plasmid

#108379

Purpose

Mammalian expression vector for xCas9 3.7

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9 3.7

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti X1 Puro DEST (694-6)

Plasmid

#17297

Purpose

3rd gen lentiviral promoter-less Gateway destination vector, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Gateway Cloning, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti-DsRed_IRES_EGFP

Plasmid

#92194

Purpose

Lentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screen

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

DsRed-IRES-EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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Integrin beta 2 - mYFP

Plasmid

#8638

Depositor

Timothy Springer

Article

Kim et al Science 2003 Sep 19;301(5640):1720-5.

Insert

Integrin beta 2 (ITGB2 Human)

Tags

YFP

Expression

Mammalian

Mutation

stop codon of beta2 was replaced with AgeI restri…

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF1a-ETV2-IRES-Puro

Plasmid

#61061

Purpose

lentiviral vector for ETV2

Depositor

Igor Slukvin

Article

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

ETV2 (ETV2 Human)

Use

Lentiviral

Promoter

EF1alpha

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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HA-HIF2alpha-P405A/P531A-pBabe-puro

Plasmid

#19006

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: P405A and P531A

Available Since

Aug. 20, 2008

Availability

Academic Institutions and Nonprofits only

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Mfn2-YFP

Plasmid

#28010

Depositor

Richard Youle

Article

Karbowski et al J Cell Biol. 2002 Dec 23. 159(6):931-8.

Insert

mitofusin 2 (MFN2 Human)

Tags

YFP

Expression

Mammalian

Mutation

silent S508S

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-HNRNPU

Plasmid

#38068

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

HNRNPU heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A) (HNRNPU Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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