4,136 results

1. Raptor_2 shRNA

   Plasmid

   #1858

   Depositor

   David Sabatini

   Article

   Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

   Insert

   Raptor (RPTOR Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK286 (mAID-NeoR)

   Plasmid

   #72824

   Purpose

   C-terminal tagging with mAID using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-Neo
   

   Use

   CRISPR

   Tags

   mAID

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 Flag Runx1 FL (P#1790)

   Plasmid

   #14585

   Depositor

   Matija Peterlin

   Article

   Jiang et al Mol Cell Biol. 2005 Dec . 25(24):10675-8

   Insert

   Runx1 (Runx1 Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-PGK-KRAS4B(G12V)

   Plasmid

   #35633

   Depositor

   Daniel Haber

   Article

   Singh et al Cell. 2012 Feb 17;148(4):639-50.

   Insert

   K-Ras4B(G12V) (KRAS Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Glycine 12 to Valine

   Promoter

   PGK

   Available since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-EGFP-Rac1(wt)

   Plasmid

   #13719

   Depositor

   Klaus Hahn

   Article

   Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

   Insert

   EGFP-Rac1(wt) (RAC1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-hULK1

   Plasmid

   #31963

   Depositor

   Do-Hyung Kim

   Article

   Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

   Insert

   ULK1 (ULK1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   Sept. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe K-Ras 12V

   Plasmid

   #12544

   Depositor

   Channing Der

   Article

   Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

   Insert

   K-Ras 12V (KRAS Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   12V constituitively activated

   Promoter

   Available since

   Sept. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. SIRT6 Flag

   Plasmid

   #13817

   Purpose

   Mammalian expression of human SIRT6 with flag tag

   Depositor

   Eric Verdin

   Article

   North et al Mol Cell. 2003 Feb . 11(2):437-44.

   Insert

   SIRT6 (SIRT6 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2-Cas9

   Plasmid

   #47322

   Purpose

   For in vivo expression of Cas9 or generation of Cas9 mRNA

   Depositor

   Alex Schier

   Article

   Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

   Insert

   Cas9
   

   Use

   CRISPR; Xenopus and zebrafish expression

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. piRFP720-N1

    Plasmid

    #45461

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP720
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

    Promoter

    CMV

    Available since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT7-EGFP-C1-HsNot1

    Plasmid

    #37370

    Depositor

    Elisa Izaurralde

    Article

    Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

    Insert

    HsNot1 (CNOT1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLM-mCerulean-cMyc

    Plasmid

    #23244

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCerulean-2A-MYC (MYC Human, synthetic fluorescent protein)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5 alpha6

    Plasmid

    #16036

    Depositor

    Filippo Giancotti

    Article

    Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

    Insert

    alpha6 (ITGA6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLL7.0: tgRFPt-SSPB WT

    Plasmid

    #60415

    Purpose

    Mammalian Expression of tgRFPt-Nano

    Depositor

    Brian Kuhlman

    Article

    Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

    Insert

    tgRFPt-SSPB
    

    Use

    Cre/Lox and Lentiviral

    Tags

    TagRFPt

    Expression

    Mammalian

    Mutation

    Y11K and A15E

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pINDUCER11 (miR-RUG)

    Plasmid

    #44363

    Purpose

    Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest

    Depositor

    Thomas Westbrook

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Insert

    PheS Gly294
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLH-spsgRNA2

    Plasmid

    #64114

    Purpose

    Vector for expression of Sp sgRNA2 in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp sgRNA2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-EZH2

    Plasmid

    #28060

    Depositor

    Mien-Chie Hung

    Article

    Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-UniSAM

    Plasmid

    #99866

    Purpose

    Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0

    Depositor

    Lesley Forrester

    Article

    Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.

    Insert

    UniSAM-mCherry + U6-gRNA2.0
    

    Use

    CRISPR and Synthetic Biology; Dcas9-sam activation

    Tags

    Expression

    Mammalian

    Mutation

    BbsI sites in CDS were ablated by consensus mutat…

    Promoter

    EF1a

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSBFP2-C1

    Plasmid

    #22880

    Depositor

    Dorus Gadella

    Article

    Kremers et al Biochemistry. 2007 Mar 27. 46(12):3775-8

    Insert

    SBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1 hygro shRNA E-cadherin

    Plasmid

    #18802

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    E-cadherin shRNA (CDH1 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge