4,177 results

1. p6XHis_NLS-SaCas9

   Plasmid

   #101086

   Purpose

   Express SaCas9 in bacteria with a 6xHis tag for purification

   Depositor

   Rick Tarleton

   Article

   Soares Medeiros et al MBio. 2017 Nov 7;8(6). pii: e01788-17. d

   Insert

   CRISPR-associated protein Cas9/Csn1
   

   Use

   CRISPR

   Tags

   TrxA-6xHis-STag

   Expression

   Bacterial

   Promoter

   T7lac

   Available Since

   Dec. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

   Plasmid

   #61592

   Purpose

   A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Has Service

   AAV6 and AAV8

   Insert

   hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Available Since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-WT-STAT1

   Plasmid

   #71454

   Purpose

   Lentiviral expression of WT-STAT1

   Depositor

   George Stark

   Article

   Cheon et al Proc Natl Acad Sci U S A. 2009 Jun 9;106

   Insert

   STAT1 (STAT1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Promoter

   CMV

   Available Since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1319 pcDNA3 flag FKHRL1 AAA

   Plasmid

   #10709

   Depositor

   William Sellers

   Article

   Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

   Insert

   FKHRL1 AAA (FOXO3 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   AAA mutant (see map) - T32A, S253A, S315A

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRsetB-PercevalHR

   Plasmid

   #49081

   Purpose

   Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

   Depositor

   Gary Yellen

   Article

   Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

   Insert

   PercevalHR
   

   Tags

   7xHis

   Expression

   Bacterial

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. DsRed-rab7 WT

   Plasmid

   #12661

   Depositor

   Richard Pagano

   Article

   Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

   Insert

   RAB7 (Homo sapiens) (RAB7A Human)
   

   Tags

   DsRed and HA

   Expression

   Mammalian

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1-hMcl-1

   Plasmid

   #25375

   Depositor

   Roger Davis

   Article

   Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

   Insert

   MCL1 (MCL1 Human)
   

   Tags

   V5 and His tag

   Expression

   Mammalian

   Available Since

   Aug. 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. mApple-pBAD

   Plasmid

   #54536

   Purpose

   Localization: Protein Expression Vector , Excitation: 568, Emission: 592

   Depositor

   Roger Tsien, Michael Davidson, Nathan Shaner

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Type

   Empty backbone

   Tags

   6xHis and mApple

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-myc3-bTrCP

   Plasmid

   #20718

   Depositor

   Yue Xiong

   Article

   Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

   Insert

   beta-transducin repeat containing (BTRC) (Btrc Mouse)
   

   Tags

   Myc3

   Expression

   Mammalian

   Mutation

   *

   Available Since

   May 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBOB-CAG-SARS-CoV2-Spike-HA

    Plasmid

    #141347

    Purpose

    3rd Generation lentiviral vector expressing the codon-optimized SARS-CoV2 Spike Glycoprotein ORF with a C-terminal HA tag generated by Junko Ogawa & Gerald M Pao

    Depositor

    Gerald Pao

    Article

    Ogawa et al bioRxiv. 2020 Jul 22. doi: 10.1101/2020.

    Insert

    SARS-CoV2 Spike Glycoprotein (S SARS-CoV2 hCoV19_USA EPI_ISL_414366 (GISAID))
    

    Use

    Lentiviral

    Tags

    HA

    Mutation

    resynthesized with human codon optimization nucle…

    Promoter

    CAG

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3TP-lux

    Plasmid

    #11767

    Depositor

    Joan Massague, Jeff Wrana

    Article

    Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

    Insert

    3TP
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Aug. 4, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. mEmerald-TOMM20-N-10

    Plasmid

    #54282

    Purpose

    Localization: Mitochondrial Membrane, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    TOMM20 (TOMM20 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. mTOR_2 shRNA

    Plasmid

    #1856

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    mTOR (MTOR Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. Stat1 alpha Flag pRc/CMV

    Plasmid

    #8691

    Depositor

    Jim Darnell

    Article

    Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

    Insert

    STAT1 alpha (STAT1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. PSD-95-pTagRFP

    Plasmid

    #52671

    Purpose

    expression of TagRFP-tagged PSD-95

    Depositor

    Johannes Hell

    Insert

    PSD-95 (Dlg4 Rat)
    

    Tags

    tagRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLPCX mito roGFP2-Orp1

    Plasmid

    #64992

    Purpose

    Mammalian expression of mitochondrial roGFP2-Orp1 (retroviral vector)

    Depositor

    Tobias Dick

    Article

    Gutscher et al J Biol Chem. 2009 Nov 13;284(46):31532-4

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Use

    Retroviral

    Tags

    roGFP2

    Expression

    Mammalian

    Mutation

    mitochondrial targeting sequence

    Available Since

    June 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. IMS-APEX2

    Plasmid

    #79058

    Purpose

    Expresses APEX in the mitochondrial intermembrane space.

    Depositor

    Alice Ting

    Article

    Hung et al Mol Cell. 2014 Jul 17;55(2):332-41. doi:

    Insert

    APEX
    

    Tags

    V5

    Expression

    Bacterial and Mammalian

    Promoter

    T7 promoter

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAVS1-TLR targeting vector

    Plasmid

    #64215

    Purpose

    Targeting vector for the human AAVS1 locus for insertion of traffic light reporter (TLR) construct

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Insert

    traffic light reporter (TLR) (AAVS1 Human)
    

    Use

    Human targeting

    Mutation

    see publication for details

    Promoter

    CAG

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. HLTV-hTRIM21

    Plasmid

    #104973

    Purpose

    Purification of His-Lipoyl-hTRIM21 protein from bacteria

    Depositor

    Leo James

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Tripartite Motif containing 21 (TRIM21 Human)
    

    Tags

    His-Lipoyl

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHPRT-DRGFP

    Plasmid

    #26476

    Purpose

    DR-GFP substrate (Addgene 26475) flanked by mouse hprt targeting sequences

    Depositor

    Maria Jasin

    Article

    Pierce et al Genes Dev. 2001 Dec 15. 15(24):3237-42.

    Insert

    pHPRT-DRGFP
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    Nov. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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