159,258 results

1. pHR-SFFV-GFP1-10

   Plasmid

   #80409

   Purpose

   Expresses GFP1-10 in mammalian cells

   Depositor

   Bo Huang

   Article

   Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

   Insert

   GFP1-10
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available since

   Dec. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZP06C

   Plasmid

   #232322

   Purpose

   pZP06C was constructed by adding the mCherry reporter gene driven by the rpsJ promoter into pZP06B (containing PrpsJ-sacB) to facilitate the screening of positive clones through red fluorescence.

   Depositor

   Chenggang Wu

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsJ

   Available since

   March 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLacO-ISce1

   Plasmid

   #58505

   Purpose

   provides 256 Lac operator repeats adjacent to the ISce1 restriction site

   Depositor

   Tom Misteli

   Article

   Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

   Insert

   Lac repeats adjacent to 6 copies of the ISce1 restriction site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   no promoter

   Available since

   Oct. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-N1-TFEB

   Plasmid

   #38119

   Purpose

   Mammalian expression of Transcription factor EB fused to EGFP

   Depositor

   Shawn Ferguson

   Article

   Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

   Insert

   TFEB (TFEB Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted stop codon

   Promoter

   CMV

   Available since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-15b-EK_C122S_His5

   Plasmid

   #49048

   Purpose

   Expresses the pentahistidine-tagged catalytic domain of bovine enteropeptidase in E. coli

   Depositor

   Hans Brandstetter

   Article

   Skala et al J Biotechnol. 2013 Dec;168(4):421-5. doi

   Insert

   Enteropeptidase, peptidase domain (TMPRSS15 Bovine)
   

   Use

   Tags

   5xHis

   Expression

   Bacterial

   Mutation

   P92R, E185D, C122S

   Promoter

   T7

   Available since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-mCherry-CDK4KTR

   Plasmid

   #126680

   Purpose

   Fluorescent reporter for CDK4/6 activity

   Depositor

   Hee Won Yang

   Article

   Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

   Insert

   RB cterm
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV4-ApoE3

   Plasmid

   #87086

   Purpose

   Expresses ApoE3 in Mammalian cells

   Depositor

   Bradley Hyman

   Article

   Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

   Insert

   Apolipoprotein E3 (APOE Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. NES-EGFP-PH-ARNO2G-I303Ex2

   Plasmid

   #116868

   Purpose

   PIP3 biosensor

   Depositor

   Gerry Hammond

   Article

   Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

   Insert

   CYTH2 (CYTH2 Human)
   

   Use

   Tags

   EGFP and X.leavis map2k1.L(32-44)

   Expression

   Mammalian

   Mutation

   amind acids 252-399, isoleucine 303 changed to gl…

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAG-FR-GECO1a

   Plasmid

   #163681

   Purpose

   Far-red fluorescent calcium indicator FR-GECO1a

   Depositor

   Robert Campbell

   Article

   Dalangin et al bioRxiv 2020.11.12.380089

   Insert

   FR-GECO1a
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-HRE-dUnaG

    Plasmid

    #124372

    Purpose

    UnaG fluorescent protein reporter for hypoxia-induced factor (HIF) signaling

    Depositor

    Roland Friedel

    Article

    Sattiraju et al Immunity. 2023 Jul 7:S1074-7613(23)00274

    Insert

    HRE-dUnaG
    

    Use

    Lentiviral

    Tags

    PEST-degron and Myc tag

    Expression

    Mutation

    Promoter

    HRE (HIF responsive element 5x + CMV minimal prom…

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-sCAG-mBaoJin-oPRE

    Plasmid

    #218684

    Purpose

    AAV vector plasmid expressing mBaoJin under a short variant of the CAG (sCAG) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.

    Depositor

    Bart Nieuwenhuis

    Insert

    mBaoJin
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Short variant of the CAG promoter

    Available since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV.TBG.PI.Cre.rBG

    Plasmid

    #107787

    Purpose

    AAV vector expressing Cre from TBG promoter

    Depositor

    James M. Wilson

    Has Service

    AAV8

    Insert

    Cre
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TBG

    Available since

    May 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFR-miRFP670nano3

    Plasmid

    #184673

    Purpose

    EGF receptor (EGFR) labelled with near-infrared fluorescent protein miRFP670nano3

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

    Insert

    EGFR-miRFP670nano3
    

    Use

    Tags

    miRFP670nano3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Luciferase-pcDNA3

    Plasmid

    #18964

    Depositor

    William Kaelin

    Article

    Safran et al Proc Natl Acad Sci U S A. 2006 Jan 3. 10

    Insert

    Firefly Luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. APEX2-NLS

    Plasmid

    #124617

    Purpose

    proximity biotinylation

    Depositor

    Alice Ting

    Article

    Kaewsapsak et al Elife. 2017 Dec 14;6. doi: 10.7554/eLife

    Insert

    APEX2-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSR43.6r

    Plasmid

    #63197

    Purpose

    Expresses CcaS, Ho1, and PcyA constitutively

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    CcaS
    Ho1
    PcyA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pscAAV-CAG-GFP

    Plasmid

    #83279

    Purpose

    recombinant AAV vector packaging self-complementary GFP under the CAG promoter

    Depositor

    Mark Kay

    Article

    Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

    Insert

    enhanced green fluorescent protein
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 TEV (full-length)

    Plasmid

    #64276

    Purpose

    Expresses tobacco etch virus protease (TEV)

    Depositor

    Xiaokun Shu

    Article

    To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

    Insert

    Tobacco etch virus (TEV) protease
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. BPK1520

    Plasmid

    #65777

    Purpose

    Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SpCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBABE-MCS(extended)-Puro (empty vector)

    Plasmid

    #172603

    Purpose

    Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    The multiple cloning site (MCS) has been edited/e…

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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