We narrowed to 85,295 results for: Mal;

1. pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)

   Plasmid

   #203911

   Purpose

   Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells

   Depositor

   Jared Toettcher

   Article

   Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

   Insert

   ZF9LOV[C2]-VP64
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)

   Plasmid

   #203910

   Purpose

   Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells

   Depositor

   Jared Toettcher

   Article

   Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

   Insert

   ZF9LOV[C1]-VP64
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   July 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRXIP-Myc-YKT6

   Plasmid

   #116945

   Purpose

   Expressing Myc-YKT6 for mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

   Insert

   Synaptobrevin homolog YKT6 (YKT6 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p330 VQR

   Plasmid

   #101730

   Purpose

   Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. p458 VRER

   Plasmid

   #101728

   Purpose

   Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E, and T1337R mutation…

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. 4xHRE_minSV40-sfGFP-CMV_dsRed

   Plasmid

   #117400

   Purpose

   sfGFP expressed from hypoxia-inducible minimal SV40 promoter and dsRed expressed from consitutive CMV promoter

   Depositor

   Yvonne Chen

   Article

   Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

   Insert

   SuperFolder GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO/Flag-tGFP-HuR.wt

   Plasmid

   #110376

   Purpose

   Mammalian expression of turboGFP-HuR (wild type) with FLAG tag, Flp-In T-Rex system

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAV like RNA binding protein 1 (ELAVL1 Human)
   

   Use

   Tags

   FLAG and tGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNICE HA-mSYD1A

   Plasmid

   #59361

   Purpose

   Expression plasmid for HA-tagged mSYD1A / Syde1 (HA-tag at N-terminus of mSYD1A)

   Depositor

   Peter Scheiffele

   Article

   Wentzel et al Neuron. 2013 Jun 19;78(6):1012-23. doi:

   Insert

   mSYD1A (Syde1 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. NKP64 GFP (1007)

   Plasmid

   #62020

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

   Insert

   SLC38A10 (SLC38A10 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

10. SlugBE4max

    Plasmid

    #163799

    Purpose

    Expresses AncBE4max and nSlugCas9

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SlugCas9 (D10A)

    Promoter

    Available since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Blat-BSD

    Plasmid

    #163791

    Purpose

    The plasmid expresses human codon-optimized BlatCas9, gRNA expression elements and blasticidin resistence gene.

    Depositor

    Yongming Wang

    Article

    Gao et al Front Cell Dev Biol. 2020 Oct 19;8:58316

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. [UBC][NRE Pum][eGFP]

    Plasmid

    #97192

    Purpose

    The wild-type Pum, fused to eGFP, and behind the ubiquitin promoter.

    Depositor

    Edward Boyden

    Article

    Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Insert

    wild type Pumilio homology domain
    

    Use

    Synthetic Biology

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    UBC (ubiquitin C)

    Available since

    Nov. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNICE HA-mSYD1B

    Plasmid

    #59362

    Purpose

    Expression plasmid for HA-tagged mSYD1B / Syde2 (HA-tag at N-terminus of mSYD1B)

    Depositor

    Peter Scheiffele

    Article

    Wentzel et al Neuron. 2013 Jun 19;78(6):1012-23. doi:

    Insert

    mSYD1B (Syde2 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTS104 pHAGE2 CMVtetO2 MCS-3C-22xGS-GFP

    Plasmid

    #199357

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAGEN-SBP-DICER1

    Plasmid

    #50558

    Purpose

    purification of Dicer1 from mammalian cells

    Depositor

    Yukihide Tomari

    Article

    Betancur et al RNA. 2012 Jan;18(1):24-30. doi: 10.1261/

    Insert

    Dicer1 (Dicer1 Mouse)
    

    Use

    Tags

    SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

16. AAV.pCry1(408).DIO.Luciferase

    Plasmid

    #192623

    Purpose

    Cre-dependent expression of firefly luciferase, driven by a minimal mouse Cry1 promoter

    Depositor

    Michael Hastings

    Article

    Brancaccio et al Science. 2019 Jan 11;363(6423):187-192.

    Insert

    luciferase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTW102

    Plasmid

    #115945

    Purpose

    ORF insert for replicon MoClo

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 DDd-L7Ae
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19-U6-crRNA4n96

    Plasmid

    #123640

    Purpose

    Mammalian expression, Genome editing, gRNA scaffold

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    crRNA scaffold 4n96
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pWZL-Stat5a1*6

    Plasmid

    #130669

    Purpose

    Retroviral vector for the expression of constitutive Stat5a (hygromycin resistance)

    Depositor

    Gerardo Ferbeyre

    Article

    Mallette et al J Biol Chem. 2007 Nov 30;282(48):34938-4

    Insert

    Stat5a (Stat5a Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Histidine 298 to Arginine and Serine 710 …

    Promoter

    LTR

    Available since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. FH95pUp95GW (B4)

    Plasmid

    #74013

    Purpose

    lentiviral expression of shRNA resistant Psd95-alpha-EGFP fusion and Psd95 shRNA

    Depositor

    Weifeng Xu, Oliver Schluter, Robert Malenka

    Article

    Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

    Insert

    Psd95 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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