We narrowed to 88,546 results for: Mal;

1. M-ST1n-VP64

   Plasmid

   #48675

   Purpose

   Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9-VP64, nuclease-null
   

   Use

   CRISPR

   Tags

   NLS and VP64

   Mutation

   nuclease-null (D9A D598A H599A N622A)

   Promoter

   CMV

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. P823_eSpCas9(1.1)-RecA

   Plasmid

   #87264

   Purpose

   Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

   Depositor

   Yonglun Luo

   Article

   Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

   Insert

   RecA
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-UBE2E1 C131A in pcDNA3.1

   Plasmid

   #118214

   Purpose

   Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

   Depositor

   Cynthia Wolberger

   Article

   Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

   Insert

   UBE2E1 (UBE2E1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed cysteine 131 to alanine

   Available Since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX-FKBP-Rab7a-Blasticidin

   Plasmid

   #120716

   Purpose

   Expresses late endosome-targeted recruiter protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   Rab7a
   

   Use

   Lentiviral

   Tags

   FKBP and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. P628_SpCas9-RecA

   Plasmid

   #87263

   Purpose

   Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

   Depositor

   Yonglun Luo

   Article

   Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

   Insert

   RecA
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   no

   Promoter

   CBh

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti6.3-SauriCas9-BlastR

   Plasmid

   #220967

   Purpose

   Expresses human codon-optimized SauriCas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   SauriCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTP924 pHAGE2 CMV GFP

   Plasmid

   #199392

   Purpose

   Lentiviral transfer plasmid for constitutive expression of a GFP in mammalian cells

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPD1028 ZF43x6-Compact(SV40Min)_EYFP

   Plasmid

   #138912

   Purpose

   EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a SV40 minimal promoter

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Insert

   ZF43x6-Compact(SV40min)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   ZF43x6-Compact(SV40min)

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

   Plasmid

   #199356

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. GWF058

    Plasmid

    #133609

    Purpose

    UAS-mCherry/CMV-Gal4DBD-NS3a-P2a-DNCR2-VPR

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    Gal4DBD-NS3a, DNCR2-VPR
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMiniP

    Plasmid

    #118049

    Purpose

    GBPart - Minimal Promoter for expression in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    minimal TATA-box promoter with low basal activity
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-HA-BRAT1

    Plasmid

    #62802

    Purpose

    Expresses human BRAT1 with an N-terminal HA tag in mammalian cells

    Depositor

    Toru Ouchi

    Article

    So et al BMC Cancer. 2014 Jul 29;14:548. doi: 10.

    Insert

    BRAT1 (BRAT1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pAAV-SMVP-Cas9N-U6-gRNA P2

    Plasmid

    #80944

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA P2 for Pd-l1 binding

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK-flag-USP19 C506S

    Plasmid

    #78584

    Purpose

    Expression flag-USP19 C506S in mammalian cells

    Depositor

    Yihong Ye

    Article

    Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

    Insert

    flag-USP19 C506S (USP19 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    mutate 506 cysteine residue to serine

    Promoter

    CMV

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

15. GWF297

    Plasmid

    #133612

    Purpose

    TET3G-2xMS2(wt+f6), NLS-MCP-ANR-ANR-P2a-BFP

    Depositor

    Dustin Maly

    Article

    Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

    Insert

    TET3G-2xMS2(wt+f6), MCP-ANR-ANR, BFP
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. px458 EQR

    Plasmid

    #101731

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-hSyn-Chronos-mT-Sapphire

    Plasmid

    #149690

    Purpose

    For packaging and expression of Chronos-mT-Sapphire in neurons via AAV

    Depositor

    Robert Malenka

    Article

    Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

    Insert

    Chronos
    

    Use

    AAV

    Tags

    mT-Sapphire

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMS2CP(V29I)-myc-T2A-tagRFP

    Plasmid

    #140260

    Purpose

    Expresses MS2CP(V29I) in mammalian cells

    Depositor

    Hirohide Saito

    Article

    Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

    Insert

    Coat protein of bacteriphage MS2
    

    Use

    Synthetic Biology

    Tags

    myc-T2A-tagRFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXPR_212

    Plasmid

    #133457

    Purpose

    U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Ef1a_NANOG_Ires_Puro

    Plasmid

    #18920

    Depositor

    Linzhao Cheng

    Article

    Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

    Insert

    NANOG (NANOG Human)
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only