We narrowed to 4,949 results for: mcs

1. macroH2A1.2-F192V-GFP (pc5115)

   Plasmid

   #223438

   Purpose

   Mutation phenylalanine 192 to valine (F192V) in macroH2A1.2-GFP

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

   Insert

   macroH2A1.2-F192V (Macroh2a1 Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Mutation of phenylalanine 192 to valine (F192V)

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGECPL.BC10.MG

   Plasmid

   #223822

   Purpose

   Contains Cas9-editable barcode, marking guide (MG) for lineage tracing, Cre for Cas9 activation and loxP-mediated gene deletion, and Firefly Luciferase (FLuc) for live luminescence-based monitoring.

   Depositor

   Dawid Nowak

   Article

   Serio et al Cancer Discov. 2024 Jul 5. doi: 10.1158/

   Insert

   Ef1a-Driven Cre-P2A-FLuc
   

   Use

   CRISPR, Cre/Lox, Lentiviral, Luciferase, and Mous…

   Tags

   P2A

   Expression

   Mammalian

   Promoter

   Ef1a

   Available Since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-IRES-blast 3xFlag-SPNS1(R76A)

   Plasmid

   #198570

   Purpose

   SPNS1(R76A) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1(R76A) with n-terminal 3xFlag (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   changed arginine R76 to alanine (R76A)

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXs-IRES-blast GFP-SPNS1(E164K)

   Plasmid

   #198572

   Purpose

   SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1(E164K) with n-terminal 3xFlag-GFP fusion (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag-GFP

   Expression

   Mammalian

   Mutation

   changed glutamic acid E164 to lysine (E164K)

   Available Since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmch-mTet1-ZF-CD pc3956

   Plasmid

   #201709

   Purpose

   Expresses of mcherry-tagged mTet1-ZF-CD in mammalian cells

   Depositor

   Cristina Cardoso

   Article

   Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

   Insert

   Tet1-ZF_CD (Tet1 Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Zinc finger domain of Tet1 fused to its catalytic…

   Promoter

   CAG

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJT309 RapInducible1-ActivatorCloneSite-Citrine

   Plasmid

   #187321

   Purpose

   Rapamycin inducible genetic circuit with cloning site for activator domains

   Depositor

   Lacra Bintu, Michael Bassik

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Hy_U5:34A eGFP SV40

   Plasmid

   #195064

   Purpose

   Expresses eGFP when there is readthrough transcription from the Drosophila U5:34A gene

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   snRNA:U5:34A (snRNA:U5:34A Fly)
   

   Expression

   Insect

   Promoter

   U5:34A

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV_dCas9-KRAB_sgSTK3i_#2 (PuroR)

   Plasmid

   #209772

   Purpose

   CRISPRi for STK3

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   dCas9-KRAB, sgSTK3i (STK3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV_dCas9-KRAB_sgSTK3i_#1 (PuroR)

   Plasmid

   #209771

   Purpose

   CRISPRi for STK3

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   dCas9-KRAB, sgSTK3i (STK3 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENeCFPCNAL2 pc922

    Plasmid

    #167563

    Purpose

    For construction of CFP-tagged human PCNA, the GFP coding sequence in the pENeGFPCNAL2 vector was replaced by the ECFP coding sequence from the pECFP-C1 vector (Clontech Laboratories, Inc., CA).

    Depositor

    Cristina Cardoso

    Article

    Zhang et al Nucleic Acids Res. 2017 Apr 25. doi: 10.

    Inserts

    Proliferating Cell Nuclear Antigen (PCNA Human)
    CFP
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgVRK2-puro

    Plasmid

    #199636

    Purpose

    sgRNA guide against VRK2

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A (VRK2 Human)
    

    Use

    Lentiviral

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX317-VRK2-K168E

    Plasmid

    #199642

    Purpose

    Expresses kinase-inactive VRK2 (K168E)

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    VRK2 (VRK2 Human)
    

    Use

    Lentiviral

    Mutation

    K168E

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgLacZ-puro

    Plasmid

    #199635

    Purpose

    sgRNA control guide targeting bacterial LacZ

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A
    

    Use

    Lentiviral

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. sgCTRL-puro

    Plasmid

    #199634

    Purpose

    sgRNA control guide targeting intergenic region

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A
    

    Use

    Lentiviral

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. sgVRK1-hygro

    Plasmid

    #199640

    Purpose

    sgRNA guide against VRK1

    Depositor

    William Hahn

    Article

    So et al JCI Insight. 2022 Oct 10;7(19):e158755.

    Insert

    N/A (VRK1 Human)
    

    Use

    Lentiviral

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSF-G1TMSNKRS

    Plasmid

    #198323

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Ekanayake et al J Med Chem. 2023 Mar 15. doi: 10.1021/ac

    Insert

    tRNA synthetase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiTet-CSK-P2A-mCherry BlastR

    Plasmid

    #186742

    Purpose

    Dox inducible CSK-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    CSK (CSK Human)
    

    Use

    Lentiviral

    Tags

    P2A-mCherry

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti_EF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP_HygroR

    Plasmid

    #186744

    Purpose

    EF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP expression construct cloned into a lentiviral backbone

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    EF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP
    

    Use

    Lentiviral

    Tags

    P2A-BFP

    Expression

    Mammalian

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM30-C9ORF78_R41A

    Plasmid

    #193368

    Purpose

    Expression of C9ORF78 R41A variant in E.coli with N-terminal His6-GST-tag which is cleavable with TEV protease

    Depositor

    Markus Wahl

    Article

    Bergfort et al Nat Commun. 2022 Mar 3;13(1):1132. doi:

    Insert

    C9ORF78_R41A (C9orf78 Human)
    

    Tags

    His6-GST-TEV

    Expression

    Bacterial

    Mutation

    R41A, the mutation is located in the binding inte…

    Promoter

    T7

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-sgNotch2#1/Cre

    Plasmid

    #193225

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch2 gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgNotch2#1 (Notch2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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