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pJC175e

Plasmid

#79219

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PPSP gIII, luxAB

Expression

Bacterial

Promoter

PPSP

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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shh10

Plasmid

#64867

Purpose

This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

Depositor

John Flannery, David Schaffer

Article

Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

Insert

shh10 cap

Use

AAV

Mutation

I319V, N451D, D532N

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pDESTtol2pACrymCherry

Plasmid

#64023

Purpose

Gateway destination vector with zebrafish aA-crystallin promoter driving mCherry GFP in lens

Depositor

Joachim Berger, Peter Currie

Article

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

mCherry

Use

Gateway Cloning

Promoter

alpha-Acrystallin promoter

Available Since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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Tol2-elavl3-GCaMP6s

Plasmid

#59531

Purpose

Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Article

Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
GCaMP6s

Available Since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/pMXs-puro

Plasmid

#51240

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Insert

3xFLAG-dCas9

Use

CRISPR and Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Expression

Mammalian

Mutation

human codon-optimized, D10A + H840A

Promoter

LTR

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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mCerulean3-C1

Plasmid

#54605

Purpose

Localization: C1 Cloning Vector, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Type

Empty backbone

Tags

mCerulean3

Expression

Mammalian

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pUltra-Chili-Luc

Plasmid

#48688

Purpose

3rd generation Lentiviral vector with bi-cistronic expression of dtomato and luciferase

Depositor

Malcolm Moore

Article

Fujiwara et al Mol Cancer Ther. 2021 Jun 4. pii: 1535-7

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pmCherry-NLS

Plasmid

#39319

Depositor

Martin Offterdinger

Article

Micutkova et al Int J Cancer. 2012 Apr 1;130(7):1544-57.

Insert

mCherry-3xNLS

Tags

3xSV40LT NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-MITF-A

Plasmid

#38132

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Sci Signal. 2012 Jun 12;5(228):ra42.

Insert

MITF-A (MITF Human)

Tags

EGFP

Expression

Mammalian

Mutation

deleted stop codon

Promoter

CMV

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only

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pBB541

Plasmid

#27394

Depositor

Bernd Bukau

Article

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

groESL (groL, groS E. coli)

Expression

Bacterial

Available Since

March 13, 2012

Availability

Academic Institutions and Nonprofits only

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p6344 pcDNA4-TO-HA-Brd4FL

Plasmid

#31351

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

Brd4FL

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

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phASCL1-N106

Plasmid

#31781

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

achaete-scute complex homolog 1 (ASCL1 Human)

Use

Lentiviral

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-hMcl-1

Plasmid

#25375

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

MCL1 (MCL1 Human)

Tags

V5 and His tag

Expression

Mammalian

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-KDR

Plasmid

#23925

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

KDR (KDR Human)

Use

Gateway Cloning

Mutation

V297I

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-oct4

Plasmid

#20323

Depositor

Rudolf Jaenisch

Article

Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

Insert

oct4 (Pou5f1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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mEGFP

Plasmid

#18696

Depositor

Karel Svoboda

Article

Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

Insert

monomeric EGFP

Expression

Mammalian

Mutation

A206K mutation

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA/Myc DeltaN TCF4

Plasmid

#16513

Depositor

Bert Vogelstein

Article

Korinek et al Science. 1997 Mar 21. 275(5307):1784-7.

Insert

TCF4 (TCF7L2 Human)

Expression

Mammalian

Mutation

Delta N: lacks aa 1-32

Available Since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pCEP4-HA-BAX

Plasmid

#16587

Depositor

Bert Vogelstein

Article

Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

Insert

BAX alpha (BAX Human)

Tags

2x HA

Expression

Mammalian

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-MKL1

Plasmid

#11978

Purpose

For mammalian expression of human MKL1 with a 3x FLAG tag

Depositor

Ron Prywes

Article

Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

Insert

MKL1 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

N579S T716A

Promoter

CMV

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only

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pKH3-human RSK1

Plasmid

#13841

Depositor

John Blenis

Article

Richards et al Mol Cell Biol. 2001 Nov . 21(21):7470-80

Insert

RSK1 (RPS6KA1 Human)

Tags

3x HA

Expression

Mammalian

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only

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