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174,630 results

Showing: 5681 - 5720 of 174630 results

  6. pCMV-SHP2 CS

    Plasmid
    #8382
    Depositor
    Ben Neel
    Insert
    SHP2 (PTPN11 Human)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    C459S, mutation of essential catalytic cysteine
    Available Since
    Sept. 26, 2005
    Availability
    Academic Institutions and Nonprofits only

  16. pKAR2-Br512

    Plasmid
    #161875
    Purpose
    Expresses Br512 polymerase in E.coli
    Depositor
    Andrew Ellington
    Article
    Maranhao et al medRxiv
    Insert
    Modified DNA Polymerase I Large Fragment (Br512)
    Tags
    8x His tag; Villin Headpiece 47 amino acid (HP47)…
    Expression
    Bacterial
    Promoter
    T7, tetPA
    Available Since
    Nov. 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    161875-plasmid-map-sequence-id-316881

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  18. pcDNA3.1_ spike_del19

    Plasmid
    #155297
    Purpose
    Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles
    Depositor
    Raffaele De Francesco
    Article
    Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2
    Insert
    SARS-Cov2 spike_deleted (S SARS-Cov2)
    Use
    Generation of sars-cov-2-pseudotyped lentiviral p…
    Mutation
    Deletion in C-term 19 aa of spike
    Promoter
    CMV
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    155297-plasmid-map-sequence-id-307858

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  20. pET_RTX-6xHis

    Plasmid
    #145029
    Purpose
    pET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tag
    Depositor
    Andrew Ellington
    Article
    Bhadra et al BioProtocol 2021
    Insert
    RTX
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145029-plasmid-map-sequence-id-292639

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  22. SpyTag003-MBP

    Plasmid
    #133450
    Purpose
    Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm
    Depositor
    Mark Howarth
    Article
    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p
    Insert
    SpyTag003-MBP
    Tags
    His6 and Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Peptide tag added to N-terminus of MBP via a GSGE…
    Promoter
    T7
    Available Since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    133450-plasmid-map-sequence-id-267039

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  23. STING-V1

    Plasmid
    #124262
    Purpose
    C-terminally tagged STING with split Venus Construct for studying localisation of STING in different cellular compartments
    Depositor
    Eric Schirmer
    Insert
    TMEM173 (STING1 Human)
    Tags
    Venus1
    Expression
    Mammalian
    Available Since
    May 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    124262-plasmid-map-sequence-id-241476

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  27. pSFV-Helper1

    Plasmid
    #92073
    Purpose
    This is a plasmid for transcription of defective helper RNA, providing structural Semliki forest virus (SFV) proteins to package recombinant SFV genomes into virus like particles.
    Depositor
    Henrik Garoff
    Article
    Liljestrom et al Biotechnology (N Y). 1991 Dec;9(12):1356
    Insert
    cDNA of SFV genome with deletion removing the nonstructural SFV proteins and also the viral RNA packaging signal.
    Use
    Helper plasmid, for run-off transcription and rna…
    Available Since
    July 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92073-plasmid-map-sequence-id-275942

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  36. R777-E283 Hs.ROCK1

    Plasmid
    #70567
    Purpose
    Gateway ORF clone of human ROCK1 [NM_005406.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    ROCK1 (ROCK1 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70567-plasmid-map-sequence-id-126016

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  38. pCDH-EF1-copGFP-T2A-Puro

    Plasmid
    #72263
    Purpose
    Express copGFP and puromycin resistance gene under EF1 promoter
    Depositor
    Kazuhiro Oka
    Insert
    copGFP-T2A-Puro
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    elongation factor-1
    Available Since
    Jan. 11, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72263-plasmid-map-sequence-id-474982

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  39. MSP2135

    Plasmid
    #72248
    Purpose
    Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    N497A, R661A, Q695A, Q926A, and D1135E
    Promoter
    CMV
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72248-plasmid-map-sequence-id-131868

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Showing: 5681 - 5720 of 174630 results