We narrowed to 9,393 results for: Coli

1. pSB1C3-J23110-B0034-mRFP1_Orange

   Plasmid

   #160444

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Orange chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

   Insert

   promoter, RBS, mRFP1E_Orange
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. mCherryS131C

   Plasmid

   #48732

   Purpose

   Expresses mCherryS131C in E. coli cells

   Depositor

   Bradley Olsen

   Article

   Thomas et al ACS Nano. 2011 Jul 26;5(7):5697-707. doi

   Insert

   mCherryS131C
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed serine 131 to cysteine

   Promoter

   T5

   Available since

   Oct. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMAL-c5X-35-H-6

   Plasmid

   #67008

   Purpose

   Expresses malE-35-H-6 in E. coli

   Depositor

   Ashutosh Chilkoti

   Article

   Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

   Insert

   malE-35-H-6
   

   Use

   Tags

   His6 and malE-Factor Xa

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Jan. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEPQD0KN0245

   Plasmid

   #162284

   Purpose

   Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven E. coli cell-free protein synthesis

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEPQDKN0729

   Plasmid

   #162316

   Purpose

   TEV protease plasmid for T7-driven E. coli cell-free protein synthesis

   Depositor

   Nicola Patron

   Article

   Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

   Insert

   T7prom-RBS-NET-TEVprotease
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-SznE

   Plasmid

   #139282

   Purpose

   expresses SznE in E. coli

   Depositor

   Emily Balskus

   Article

   Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

   Insert

   sznE
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMOD4 galK-GT

   Plasmid

   #19183

   Depositor

   Al Fisher

   Article

   Zhang et al BMC Dev Biol. 2008 Dec 30;8(1):119.

   Insert

   galK (galK E.coli)
   

   Use

   Recombineering

   Tags

   50 nucleotides from N-term TAP, C-term TAP, and F…

   Expression

   Mutation

   Promoter

   Available since

   Jan. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28a-PAN

   Plasmid

   #200971

   Purpose

   Express poly-histidine tagged Methanococcus jannaschii PAN in E. coli recombinantly

   Depositor

   Yifan Cheng

   Article

   Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

   Insert

   PAN
   

   Use

   Tags

   6xHis and 6xHis-thrombin site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBbS5c-AcrB/AcrD/AcrFa, ABO_0964

   Plasmid

   #86206

   Purpose

   Expresses efflux pump in E. coli to provide resistance to biofuel (low copy)

   Depositor

   Mary Dunlop

   Article

   Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

   Insert

   Efflux pump
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

   Available since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28a-hdNadV

    Plasmid

    #83362

    Purpose

    Expresses nadV from Haemophilus ducreyi in Escherichia coli

    Depositor

    George Cătălin Marinescu

    Article

    Marinescu et al Sci Rep. 2018 Mar 13;8(1):4433. doi: 10.

    Insert

    nadV
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pND004

    Plasmid

    #200950

    Purpose

    MBP-tagged E. coli protein expression destination vector for Golden Gate cloning

    Depositor

    Timothy Whitehead

    Article

    Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

    Type

    Empty backbone

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEDL3

    Plasmid

    #25092

    Depositor

    Christopher Voigt

    Article

    Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

    Insert

    Edge detector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAW-SpyCsn2

    Plasmid

    #113251

    Purpose

    E. coli expression vector for S. pyogenes Csn2-MBP-His

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    S. pyogenes Csn2
    

    Use

    Tags

    MBP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSETb-pHlash

    Plasmid

    #61550

    Purpose

    Fusion of Rluc8 and cpVenus. Expression of pHlash in Escherichia coli for protein purification

    Depositor

    Carl Johnson

    Article

    Zhang et al PLoS One. 2012;7(8):e43072. doi: 10.1371

    Insert

    pHlash
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pAW-SpyCas1

    Plasmid

    #113248

    Purpose

    E. coli expression vector for His-MBP-S. pyogenes Cas1

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    S. pyogenes Cas1
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAW-SpyCas2

    Plasmid

    #113249

    Purpose

    E. coli expression vector for His-MBP-S. pyogenes Cas2

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    S. pyogenes Cas2
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRD324

    Plasmid

    #198045

    Purpose

    Expression of HMfB D14A K30A E34A in E. coli

    Depositor

    Remus Dame

    Article

    Erkelens et al Front Microbiol. 2023 Apr 18;14:1166608.

    Insert

    HMfB D14A K30A E34A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pet11a-ABLE

    Plasmid

    #158627

    Purpose

    Amp resistant IPTG inducible 6xHis-TEV-ABLE protein for E. coli expression

    Depositor

    William DeGrado

    Article

    Polizzi et al Science. 2020 Sep 4;369(6508):1227-1233.

    Insert

    Apixaban-Binding Helical Bundle
    

    Use

    Tags

    6x His-tag and TEV cleavage tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcrRNA.ind

    Plasmid

    #61284

    Purpose

    Arabinose-inducible repeat-only E. coli CRISPR array

    Depositor

    Chase Beisel

    Article

    Luo et al Nucleic Acids Res. 2014 Oct 17. pii: gku

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    Dec. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJT105

    Plasmid

    #25089

    Depositor

    Christopher Voigt

    Article

    Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

    Insert

    Edge detector delta cI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMAL-c5X-ELP[V5A2G3-60]

    Plasmid

    #67009

    Purpose

    Expresses malE-ELP[V5A2G3-60] in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-ELP[V5A2G3-60]
    

    Use

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCDW114

    Plasmid

    #70061

    Purpose

    artificial transcription template "wild-type"

    Depositor

    Jeff Gelles

    Article

    Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

    Insert

    lambda PR' - repeat cassette - E. coli rpoB - lambda TR'
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

23. pGEX-6P-1-GST-CD-ATL (Cbx7)

    Plasmid

    #82527

    Purpose

    Expresses mutant GST-CD-ATL (Cbx7) fusion proteins in E. coli. Cbx7 CD-ATL region, amino acids 1–84.

    Depositor

    Xiaojun Ren

    Insert

    Chromobox Homolog 7 (CBX7 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 1–84

    Promoter

    Available since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEXndoE

    Plasmid

    #47714

    Purpose

    Expresses GST-EndoE from E. faecalis in E. coli

    Depositor

    Vincent Fischetti, Mattias Collin

    Article

    Collin et al J Biol Chem. 2004 May 21;279(21):22558-7

    Insert

    ndoE
    

    Use

    Tags

    Glutathione S-transferase

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHL 2057

    Plasmid

    #64909

    Purpose

    AmpR + ColE1 + PLtetO-1::RBS(st7):cro::cII (31 basepairs)::T1 terminator + PLlacO-1::RBS(st7):psu::T1 terminator

    Depositor

    Han Lim

    Article

    Hussein et al Biochim Biophys Acta. 2015 May 14;1849(8

    Type

    Empty backbone

    Use

    Synthetic Biology; E. coli

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pUC57-FASTKD4

    Plasmid

    #73620

    Purpose

    Cloning vector containing FASTKD4

    Depositor

    Vamsi Mootha

    Article

    Wolf et al Cell Rep. 2014 May 8;7(3):918-31. doi: 1

    Insert

    FASTKD4 (TBRG4 Human)
    

    Use

    Tags

    Expression

    Mutation

    FASTKD4 has been codon optimized for expression i…

    Promoter

    lac

    Available since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

27. pHL 2067

    Plasmid

    #64910

    Purpose

    AmpR + ColE1 + PLtetO-1:: extra 5’UTR::RBS(st7):cfp::T1 terminator + PLlacO-1::RBS(st7):psu::T1 terminator

    Depositor

    Han Lim

    Article

    Hussein et al Biochim Biophys Acta. 2015 May 14;1849(8

    Type

    Empty backbone

    Use

    Synthetic Biology; E. coli

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pPLP1.3

    Plasmid

    #22615

    Depositor

    Lynn Hudson

    Article

    Puckett et al J Neurosci Res. 1987 . 18(4):511-8.

    Insert

    Proteolipid Protein
    

    Use

    E. coli phagemid vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

29. I766T USP7 UBL-12

    Plasmid

    #227232

    Purpose

    Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    I766T; aa 535-775

    Promoter

    T7-promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. L757P USP7 UBL-12

    Plasmid

    #227231

    Purpose

    Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    L757P; aa 535-775

    Promoter

    T7-promoter

    Available since

    April 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28_Strep-Psc-pScaC

    Plasmid

    #228834

    Purpose

    E. coli expression of the ScaC passenger domain

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    ScaC
    

    Use

    Tags

    Strep

    Expression

    Bacterial

    Mutation

    aa 33-223

    Promoter

    Available since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pET28_pScaC(50-159)

    Plasmid

    #228836

    Purpose

    E. coli expression of a truncated ScaC passenger domain (aa 50-159)

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    ScaC
    

    Use

    Tags

    Strep

    Expression

    Bacterial

    Mutation

    aa 50-159

    Promoter

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. R152C USP7 TRAF

    Plasmid

    #227229

    Purpose

    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    R152C; aa 62-205

    Promoter

    T7-promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. G392A FL USP7

    Plasmid

    #227217

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    G392A

    Promoter

    T7-promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. L469R USP7 catalytic

    Plasmid

    #227212

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    L469R; aa 207-560

    Promoter

    T7-promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET27b+R

    Plasmid

    #188245

    Purpose

    Empty backbone vector for protein expression in Escherichia coli and his-tag purification

    Depositor

    Yiping Qi

    Article

    Fang et al Methods Mol Biol. 2023;2653:153-171. doi

    Type

    Empty backbone

    Use

    Tags

    6xHis tag and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJExpress401-T4 Lysozyme C54T C97A (WT*)

    Plasmid

    #224353

    Purpose

    Expresses T4 lysozyme in E. coli

    Depositor

    Terry Watt

    Article

    Toro et al MethodsX. 2015 May 18;2:256-62. doi: 10.

    Insert

    T4 Lysozyme
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Bacterial

    Mutation

    C45T, C94A

    Promoter

    T5

    Available since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pBz84

    Plasmid

    #226318

    Purpose

    E. coli protein expression for protein purification

    Depositor

    David Savage

    Article

    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

    Insert

    6xHis- (wtMR1-6)- strep
    

    Use

    Tags

    6xHis and Strep

    Expression

    Bacterial

    Mutation

    N-terminal domain deleted, C-terminal domain dele…

    Promoter

    T7

    Available since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. N418S FL USP7

    Plasmid

    #227215

    Purpose

    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    N418S

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. H111Q USP7 TRAF

    Plasmid

    #227227

    Purpose

    Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Use

    Tags

    6His

    Expression

    Bacterial

    Mutation

    H111Q; aa 62-205

    Promoter

    T7-promoter

    Available since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge