We narrowed to 12,028 results for: LEA

Showing: 2841 - 2860 of 12028 results

Results per page:

Sort results by:

mCherry Codon 59 sgRNA

Plasmid

#109431

Purpose

MLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporter

Depositor

Reuben Harris

Article

St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

Insert

mCherry codon 59 gRNA

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJRTCLCrVB1.3

Plasmid

#22844

Depositor

Niki Robertson

Article

Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

Insert

B DNA

Use

Cotton vigs vector

Expression

Bacterial

Mutation

1.3 unit B componet length inserted into pBlueScr…

Available Since

March 16, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
SpCas9-NG-YE1-BE4max

Plasmid

#198535

Purpose

Expresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NG-YE1-BE4max

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet-1_VNp-mNeongreen_OmpA-mCherry

Plasmid

#182390

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen and ompA-mCherry fusions

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-mNeongreen-His
OmpA-mCherry

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-3C-sGFP1-10_pcDNA3.1(-)

Plasmid

#182931

Purpose

For expression of membrane protein with N-terminal FLAG tag and C-terminal split GFP1-10 fragment

Depositor

William Weis

Article

Bruguera et al J Biol Chem. 2022 Jan 21:101628. doi: 10

Type

Empty backbone

Tags

3C-cleavable split GFP 1-10, FLAG tag, and HA sig…

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSHS306 - Bacterial expression plasmid for SpCas9, HNH FRET variant

Plasmid

#87350

Purpose

S867C/S355C in cysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmid, HNH-1 FRET construct

Depositor

Jennifer Doudna

Article

Sternberg et al Nature. 2015 Nov 5;527(7576):110-3. doi:

Insert

Cas9

Tags

His-MBP

Expression

Bacterial

Mutation

C80S/S355C/C574S/S867C

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
6974 pPROEX-HTb-SSB1

Plasmid

#1232

Depositor

Susan Lindquist

Article

Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

Insert

ssb1 (SSB1 Budding Yeast)

Tags

his tag (tev cleavable)

Expression

Bacterial

Available Since

Aug. 9, 2005

Availability

Academic Institutions and Nonprofits only
pSH-Csy4-T2A-SaRFN

Plasmid

#85755

Purpose

Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Backbone derived from pSQT1601 (Addgene #53369).

Depositor

Lawrence Stanton

Article

Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

Insert

Csy4-T2A-FokI-SadCas9

Use

CRISPR

Tags

3xHA tag, Csy4, and FokI fusion via 36 amino acid…

Expression

Mammalian

Mutation

D10A, N580A

Promoter

CAG

Available Since

Jan. 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-EF1a-DIO-GRAB_g5-HT3.0

Plasmid

#208726

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0

Use

AAV

Promoter

EF1a

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDisplay-NE2m-IRES-mCherry-CAAX

Plasmid

#208690

Purpose

Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2m and a membrane-localized mcherry in mammalian cells

Depositor

Yulong Li

Article

Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

Insert

GPCR activation based norepinephrine (NE) sensor GRAB_NE2m

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pX601-AAV-CMV-NLS-ISYmu1-NLS-3xHA-bGHpA;U6-BsaI-reRNA

Plasmid

#205317

Purpose

All-in-one AAV vector expressing ISYmu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

humanized ISYmu1 TnpB

Use

AAV

Tags

3 x HA

Expression

Mammalian

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHS0806 His14-bdSUMO-AwaIscB

Plasmid

#176539

Purpose

Bacterial expression of His14-bdSUMO-AwaIscB

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

AwaIscB

Use

CRISPR

Tags

14xHis-bdSUMO tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-hSyn-DIO-mCReP

Plasmid

#167503

Purpose

Conditional expression of the eIF2alpha phophatase, CReP (Ppp1r15b)

Depositor

Nicole Calakos

Article

Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

Insert

Ppp1r15b (Ppp1r15b Mouse)

Use

AAV and Cre/Lox

Tags

Myc

Expression

Mammalian

Promoter

hSyn

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 AIF-mClover

Plasmid

#129575

Purpose

Expression of mClover fused AIF

Depositor

Stephen Tait

Article

Riley et al EMBO J. 2018 Sep 3;37(17). pii: embj.201

Insert

AIF (AIFM1 Human)

Expression

Mammalian

Mutation

None

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-ExRai-AKAR-NES

Plasmid

#118407

Purpose

Cytosol-targeted excitation-ratiometric biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AKAR-NES

Tags

Nuclear export signal (NES)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNCS Citrine

Plasmid

#91761

Purpose

Bacterial expression of a fluorescent protein, Citrine

Depositor

Erik Rodriguez, Roger Tsien

Article

Griesbeck et al J Biol Chem. 2001 Aug 3;276(31):29188-94

Insert

Citrine

Tags

Hexa-Histidine tag, Xpress epitope for detection …

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-SynI-CreOn-FlpOff-TeNT-P2A-EGFP

Plasmid

#176282

Purpose

Viral vector for co-expression of TeNT and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

Depositor

Martin Riccomagno

Article

Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

TeNT-P2A-EGFP

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

human Synapsin I

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Diacylglycerol Kinase Zeta

Plasmid

#85454

Purpose

expression of Diacylglycerol Kinase Zeta

Depositor

Stephen Gee

Article

Hogan et al J Biol Chem. 2001 Jul 13;276(28):26526-3

Insert

Diacylglycerol Kinase Zeta (DGKZ Human)

Tags

3x HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pVP2A-PIF6

Plasmid

#73369

Purpose

Expresses Adeno-associated virus VP2 with PIF6 insert.

Depositor

Junghae Suh

Article

Gomez et al ACS Nano. 2015 Nov 30.

Insert

PIF6 (PIL2 Mustard Weed)

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)-Mito-CaNARi

Plasmid

#64851

Purpose

Mitochondria-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of mitochondrial outer membrane.

Depositor

Jin Zhang

Article

Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

Insert

CaNARi

Tags

Cerulean, N-terminal 30 amino acids from DAKAP1, …

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge