We narrowed to 14,578 results for: ung

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pDSM-ef-Ppfy1-CSC-Taip1

Plasmid

#127741

Purpose

Yeast pathway position 6. CSC transcription unit with the PFY1 promoter and AIP1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ppfy1

Availability

Academic Institutions and Nonprofits only

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pDSM-ef-Psbtdh3-CSC-Tefm1

Plasmid

#127742

Purpose

Yeast pathway position 6. CSC transcription unit with the S. bayanus TDH3 promoter and EFM1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

citrate synthase

Use

Synthetic Biology

Expression

Yeast

Promoter

Psbtdh3

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Peno2-ACDH-Ttal1

Plasmid

#127743

Purpose

Yeast pathway position 7. ACDH transcription unit with the ENO2 promoter and TAL1 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Peno2

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Pvma6-ACDH-Trps14a

Plasmid

#127744

Purpose

Yeast pathway position 7. ACDH transcription unit with the VMA6 promoter and RPS14A terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Pvma6

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Prpl28-ACDH-Tecm10

Plasmid

#127745

Purpose

Yeast pathway position 7. ACDH transcription unit with the RPL28 promoter and ECM10 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Prpl28

Availability

Academic Institutions and Nonprofits only

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pDSM-f3-Ptdh3-ACDH-Trpl3

Plasmid

#127746

Purpose

Yeast pathway position 7. ACDH transcription unit with the TDH3 promoter and RPL3 terminator.

Depositor

Christopher Voigt

Article

Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

Insert

acetylating acetaldehyde dehydrogenase

Use

Synthetic Biology

Expression

Yeast

Promoter

Ptdh3

Availability

Academic Institutions and Nonprofits only

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HER2 WT

Plasmid

#16257

Depositor

Mien-Chie Hung

Article

Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

Insert

HER2 (ERBB2 Human)

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag-MBD1 R44Q-14-mVenusC

Plasmid

#191394

Purpose

R44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

pcDNA3 Flag-MBD1 R44Q-14-mVenusC

Tags

3xFLAG

Expression

Mammalian

Mutation

R44Q

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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lentiSAMv2

Plasmid

#75112

Purpose

lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Article

Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EF1A

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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pCH45 (multiAsCas12a-KRAB lenti)

Plasmid

#217330

Purpose

Lentiviral expression of multiAsCas12a-KRAB

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

multiAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Mutation

R1226A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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HA-Vps34

Plasmid

#86749

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

human Vps34 (PIK3C3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGIPz-PD-L1 WT

Plasmid

#121486

Purpose

2nd generation lentiviral vector for over expressing human PD-L1 WT with PD-L1 shRNA

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

PD-L1

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2

Plasmid

#215327

Purpose

Lentiviral mammalian vector with firefly luciferase reporter for MUC5AC expression; renilla luciferase and dsRed2 reporter under CMV promoter.

Depositor

Rob Hynds

Article

Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

Insert

MUC5AC Promoter

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

MUC5AC

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pKeima-LiveDrop

Plasmid

#199695

Purpose

lipophagy reporter system based on the Keima-fluorophore

Depositor

Tobias Walther

Article

Chung et al Nat Cell Biol. 2023 Aug;25(8):1101-1110.

Insert

GPAT4 (Gpat4 Fly)

Tags

Keima

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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HER2 CA (V659E)

Plasmid

#16259

Depositor

Mien-Chie Hung

Article

Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

Insert

HER2 (ERBB2 Human)

Expression

Mammalian

Mutation

Constitutively active: V659E

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1

Plasmid

#233078

Purpose

Expression vector for sgRNA-S1b and eCas12f1

Depositor

Kyoungmi Kim

Article

Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

Insert

U6-sgRNA-S1b (BsaI)-CBh-bpNLS-eCas12f1-2xFLAG-P2A-EGFP

Use

CRISPR

Tags

2xFLAG

Expression

Mammalian

Promoter

hU6, CBh

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_HsELOB

Plasmid

#254348

Purpose

Expression of ELOB for protein purification

Depositor

David Bartel

Article

Farnung et al Nature. 2026 Apr;652(8110):784-793. doi:

Insert

Human Elongin B (ELOB Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pCI-Caspase 1

Plasmid

#41552

Depositor

Kate Fitzgerald

Article

Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

Insert

Caspase-1 (CASP1 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV immediate-early enhancer/promoter

Available Since

Dec. 20, 2012

Availability

Academic Institutions and Nonprofits only

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BPK764

Plasmid

#65767

Purpose

Bacterial expression plasmid for SpCas9 & sgRNA (need to clone spacer into BsaI sites): T7-humanSpCas9-NLS-3xFLAG-T7-BsaIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9-NLS-3XFlag, and SpCas9 gRNA

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Bacterial

Promoter

T7 (x2)

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pDC216

Plasmid

#115791

Purpose

Expresses rat Syntaxin 1

Depositor

Axel Brunger

Article

Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

Insert

Syntaxin1 (Stx1a Rat)

Tags

hexa histidine

Expression

Bacterial

Mutation

C145S S193C C271S C272S

Promoter

T7

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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