We narrowed to 13,951 results for: CRISPR-Cas9

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pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-Blast

Plasmid

#71219

Purpose

dCas9 fused to the human DNMT3B catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-DNMT3B(E697A), DNMT3B catalytic domain with inactivation mutation E697A

Use

Lentiviral

Tags

dSpCas9-DNMT3B(E697A)

Expression

Mammalian

Available Since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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AOI-WT-Cas9-sg-mouse Gfi1-exon5 (F3)-GFP

Plasmid

#91884

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Gfi1-exon4(F2)-GFP

Plasmid

#91877

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
AOI-WT-Cas9-sg-mouse Gfi1-exon4(F1)-GFP

Plasmid

#91883

Purpose

Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

Depositor

Martine Roussel

Article

Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

Insert

gRNA targeting Gfi1 (Gfi1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only
AAV_NLS-dSaCas9-NLS-VPR

Plasmid

#68495

Purpose

AAV vector containing nuclease null SaCas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

dSaCas9

Use

AAV

Tags

VPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pET-dCas9-VP64-6xHis

Plasmid

#62935

Purpose

Expression of dCas9-VP64-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

dCas9-VP64

Tags

3xFLAG, 6xHis, NLS, and VP64

Expression

Bacterial

Mutation

D10A and H840A amino acid changes render Cas9 nuc…

Promoter

T7

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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LLP339 pGK-dCas9-SunTag

Plasmid

#100943

Purpose

dCas9 vector with SunTagx10, driven by pGK, with 3xHA tag, Puro driven by EF1a

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

dCas9, SunTag array (10xGCN4)

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Mutation

D10A, H840A for dCas9

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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AAVi U6_gRNA CMV_SadCas9-KRAB

Plasmid

#214609

Purpose

All-in-one AAVi plasmid expressing S. aureus dCas9-KRAB with sgRNA cassette

Depositor

Ralf Gilsbach

Article

Laurette et al Circ Res. 2023 Dec 22. doi: 10.1161/CIRC

Inserts

gRNA
SadCas9

Use

AAV

Tags

NP NLS and SV40 NLS

Expression

Mammalian

Mutation

D10A, N580A

Promoter

CMV and U6

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9-P2A-Puro

Plasmid

#110837

Purpose

Lentiviral vector for expression of Cas9 in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

Cas9

Use

Lentiviral

Mutation

WT

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pSaCas9-1xms2-2x3’UTR

Plasmid

#122946

Purpose

For expressing SaCas9 mRNA with one copy of MS2 aptamer and 2 copies of HBB 3' UTR

Depositor

Baisong Lu

Article

Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

Insert

SaCas9-MS2-HBB 3' UTR

Use

AAV

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSaCas9-1xPP7-2x3'UTR

Plasmid

#122947

Purpose

For expressing SaCas9 mRNA with one copy of PP7 aptamer and 2 copies of HBB 3' UTR

Depositor

Baisong Lu

Article

Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

Insert

SaCas9-PP7-HBB 3' UTR

Use

AAV

Expression

Mammalian

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSB-XPRESSO-hSpCas9-EGFP

Plasmid

#237298

Purpose

Sleeping Beauty XPRESSO vector for Cas9-EGFP expression

Depositor

Lior Gepstein

Article

Wexler et al Stem Cell Reports. 2025 Aug 19:102603. d

Insert

spCas9 (cas9 Stretococcus pyogenes)

Use

CRISPR

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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gRNA3_NIPBL_Cas9-hGem-for_N-terminal_tagging

Plasmid

#217662

Purpose

expresses Cas9-hGem and guideRNA for N terminal tagging of NIPBL

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

sgRNA3 for N terminal tagging of NIPBL (NIPBL Human)

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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AAV_NLS-SaCas9-NLS-VPR

Plasmid

#68496

Purpose

AAV vector containing SaCas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

SaCas9-VPR

Use

AAV and CRISPR

Tags

VPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-mR2-ACTB_sgRNA

Plasmid

#183884

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ACTB sgRNA spacer (ACTB Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR221-H1-sgGFP1-7SK-sgCas9

Plasmid

#87908

Purpose

multiple sgRNA

Depositor

Nicole Déglon

Article

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Insert

sgGFP1, sgCas9

Use

Gateway entry vector

Promoter

H1, 7SK

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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px458-Cas9 UPF1 sgRNA

Plasmid

#251491

Purpose

Cas9 CRISPR gRNA construct; expresses human UPF1 gRNA

Depositor

Markus Landthaler

Article

Boehm et al Mol Cell. 2025 Sep 18;85(18):3524-3546.e

Insert

UPF1 gRNA

Expression

Bacterial

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pX330-U6-E690_guide-CBh-hSpCas9

Plasmid

#188545

Purpose

Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid E690

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

gRNA

Use

CRISPR

Tags

T2A-EGFP

Promoter

U6

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-U6-W308_guide-CBh-hSpCas9

Plasmid

#188546

Purpose

Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid W308

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

gRNA

Use

CRISPR

Tags

T2A-EGFP

Promoter

U6

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-U6-L101_guide-CBh-hSpCas9

Plasmid

#188547

Purpose

Plasmid encoding pCas9 and gRNA for mutagenesis or gene-correction of human ABCA3 mutation at amino acid L101

Depositor

Darrell Kotton

Article

Sun et al J Clin Invest. 2024 Jan 16;134(2):e16427

Insert

gRNA

Use

CRISPR

Tags

T2A-EGFP

Promoter

U6

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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