We narrowed to 7,345 results for: Lif;

1. pCA528 CHMP7 366-453

   Plasmid

   #104598

   Purpose

   Expresses C-terminal 366-453 residues of CHMP7 with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-12

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   CHMP7 residues 366-453 (CHMP7 Human)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRXIP-GFP-TAPP1(PHD)

   Plasmid

   #221757

   Purpose

   Stable expression of GFP-TAPP1(PHD) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   PLEKHA1 (PLEKHA1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   tandem TAPP1 (PHD) (amino acids 184-304)

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRXIP-Btk1(PHD)-GFP

   Plasmid

   #221756

   Purpose

   Stable expression of Btk1(PHD)-GFP in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   Btk (BTK Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   amino acids 1–177

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRXIP-GFP-ING2(PHD)

   Plasmid

   #221751

   Purpose

   Stable expression of GFP-ING2(PHD) in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   ING2 (ING2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   two ING2(PlantHD) domains (amino acids 190–280)

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-XPACK-DNAJC5 (L115R)- HaloTag

   Plasmid

   #205712

   Purpose

   Mammalian expression of XPACK-DNAJC5 (L115R)-HaloTag

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (DNAJC5 Human)
   

   Use

   Tags

   HaloTag and XPACK (GCINSKRKD)

   Expression

   Mammalian

   Mutation

   L115R

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-DEAD XPACK-DNAJC5 (L115R)

   Plasmid

   #205718

   Purpose

   Mammalian expression of DEAD XPACK-DNAJC5 (L115R)

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (DNAJC5 Human)
   

   Use

   Tags

   Dead XPACK (GCINIKRKD)

   Expression

   Mammalian

   Mutation

   L115R

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE-80L 2xHis-LRRK2 Armadillo-K17A

   Plasmid

   #186019

   Purpose

   2xHis-LRRK2 Armadillo-K17A

   Depositor

   Suzanne Pfeffer

   Article

   Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

   Insert

   2xHis-LRRK2 Armadillo-K17A (LRRK2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE-80L 2xHis-LRRK2 Armadillo- K18A

   Plasmid

   #186020

   Purpose

   2xHis-LRRK2 Armadillo- K18A

   Depositor

   Suzanne Pfeffer

   Article

   Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

   Insert

   2xHis-LRRK2 Armadillo- K18A (LRRK2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV Flag-LRRK2 K17A/K18A

   Plasmid

   #186012

   Purpose

   Flag-LRRK2 K17A/K18A

   Depositor

   Suzanne Pfeffer

   Article

   Vides et al Elife. 2022 Sep 23;11:e79771. doi: 10.75

   Insert

   Flag-LRRK2 K17A/K18A (LRRK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AAV1-DIO-eGFP-pvRPL10a

    Plasmid

    #202542

    Purpose

    Cr-dependent (DIO) expression of eGFP-tagged ribosomal subunit that uses the prairie vole RPL10a gene sequence under the human synapsin promoter.

    Depositor

    Zoe Donaldson

    Article

    Sadino et al Elife. 2023 Feb 28;12:e80517. doi: 10.75

    Insert

    RPL10a subunit of the ribosome
    

    Use

    AAV and Cre/Lox

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    optimized for prairie vole DNA sequence and optim…

    Promoter

    human synapsin

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-CHMP2A-myc, L216D/L219D

    Plasmid

    #180645

    Purpose

    Mammalian expression of CHMP2A with L216D/L219D mutations. Has C-terminal Myc tag. Internal ID:WISP20-13.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    L216D, L219D

    Promoter

    pCAG

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-OSF-KATNA1 R14A

    Plasmid

    #160052

    Purpose

    Mammalian expression vector for KATNA1 (KATANIN-P60) R14A mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-19.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Tags

    One-Strep-Flag (OSF)

    Expression

    Mammalian

    Mutation

    R14A mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-OSF-KATNA1 V55D

    Plasmid

    #160053

    Purpose

    Mammalian expression vector for KATNA1 (KATANIN-P60) V55D mutant with N-terminal One-Strep-Flag tag. Internal ID: WISP20-17.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Tags

    One-Strep-Flag (OSF)

    Expression

    Mammalian

    Mutation

    V55D mutation

    Promoter

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCA528 KATNA1 MIT residues 1-79, V55D

    Plasmid

    #180615

    Purpose

    Bacterial expression for KATNA1 MIT domains, residues 1-79. Has V55D mutation. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-23.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    KATNA1 (KATNA1 Human)
    

    Use

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 1-79, V55D mutation

    Promoter

    T7

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-π-EB1_IRES-mCherry

    Plasmid

    #197427

    Purpose

    Homology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    IRES-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GCN4 leucine zipper, LOV2, Zdk1, and mCherry

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-π-EB1_EF1α-mCherry

    Plasmid

    #197429

    Purpose

    Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    GCN4 leucine zipper, LOV2, Zdk1, and mCherry

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC19-π-EB1_EF1α-EGFP

    Plasmid

    #197430

    Purpose

    Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5

    Depositor

    Torsten Wittmann

    Article

    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

    Insert

    EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    EGFP, GCN4 leucine zipper, LOV2, and Zdk1

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-INSRR-V5/HIS

    Plasmid

    #201981

    Purpose

    expression of human INSRR receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    INSRR receptor tyrosine kinase (INSRR Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lenti-guide-puro mCct8 - 2

    Plasmid

    #198504

    Purpose

    lentiviral stable expression of mCct8 gRNA 2

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mCct8 (Cct8 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-guide-puro mAtp5c-1

    Plasmid

    #198479

    Purpose

    lentiviral stable expression of mAtp5c gRNA 1

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    mAtp5c (Atp5c1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge