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pRG711_lexO-AscI_LexA-TF_HIS3MX

Plasmid

#154820

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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p6721 MSCV-IP N FlagHA 16E6 8S9A10T

Plasmid

#44153

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16E6

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

E6 8S9A10T mutant

Promoter

MSCV LTR

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pSUM36-MCS2-myc

Plasmid

#109379

Purpose

Shuttle vector for high level expression in mycobacteria.

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

MCS2-Myc

Tags

Myc

Expression

Bacterial

Promoter

Kanamycin

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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p6722 MSCV-IP N FlagHA 16E6 I128T

Plasmid

#44154

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16E6

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

HPV16 E6 I128T mutant

Promoter

MSCV LTR

Available Since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
pFUW-tetO-Nfat1

Plasmid

#139830

Purpose

doxycycline-inducible expression of mouse Nfat1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Nfat1

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNAflaB

Plasmid

#149642

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi flaB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAflaB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-Dm4E-T

Plasmid

#79261

Purpose

Expresses EGFP-tagged Dm4E-T in S2 cells

Depositor

Elisa Izaurralde

Article

Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

Insert

Dm4E-T (4E-T Fly)

Use

Schneider 2 cell expression

Tags

EGFP

Promoter

Ac5 promoter

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNAmreB

Plasmid

#149653

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-ABEmax(TadA E59A, TadA*E59A)

Plasmid

#125649

Purpose

pCMV-ABEmax(TadA E59A, TadA*E59A) in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

ABEmax(TadA E59A TadA* E59A)

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pQTEV-PHYHIP

Plasmid

#31578

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

phytanoyl-CoA hydroxylase interacting protein (PHYHIP Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA

Plasmid

#133344

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_cpaA

Use

CRISPR

Promoter

constitutive

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pNP1 OR sfGFP

Plasmid

#107364

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 OR input A and pNP1 OR input B are co-expressed

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

OR gate + sfGFP

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_arr2

Plasmid

#149639

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pJL1-PorA-4xDQNAT

Plasmid

#128392

Purpose

In vitro expression of N. meningitidis PorA with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

PorA

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJL1-TTc-4xDQNAT

Plasmid

#128393

Purpose

In vitro expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Insert

Tetanus toxin fragment C domain

Tags

4xDQNAT glycosylation tag and 6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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pJKW1847

Plasmid

#174004

Purpose

Expresses PpCYP94D108 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

Depositor

Jing-Ke Weng

Article

Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

Inserts

PpCYP94D108
ATR1 (ATR1 Mustard Weed)

Expression

Yeast

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK-kinase10

Plasmid

#59123

Purpose

FAK fret control sensor without FERM domain with 10 nm linker

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2_BirA

Plasmid

#136521

Purpose

Used as a donor vector to clone into pSLIK

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

BirA

Use

Entry vector for gateway cloning

Mutation

R118G (highly promiscuous form)

Available Since

June 26, 2021

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6C-(928-1020)-NES-mut

Plasmid

#42027

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6C (TNRC6C Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa928-1020, L943A, L947A, M950A, F952A

Promoter

CMV

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pLX-V5-TRAF2 S102A-puro

Plasmid

#44113

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S102A (TRAF2 Human)

Use

Lentiviral

Tags

V5

Mutation

Serine 102 to Alanine

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only
pDD2002

Plasmid

#101198

Purpose

Name: pCBG68-LANApi. LANApi driving green luciferase reporter for CBG68 in pCBG68 backbone.

Depositor

Dirk Dittmer

Article

Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

Insert

LANApi driving green luciferase reporter for CBG68 in pCBG68 backbone.

Use

Luciferase

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pDD2005

Plasmid

#101219

Purpose

Name: pCBR-K14. K14 driving red luciferase reporter for CBR in pCBR backbone.

Depositor

Dirk Dittmer

Article

Hilton et al J Virol. 2012 Sep;86(18):9683-95. doi: 1

Insert

K14 driving red luciferase reporter for CBR in pCBR backbone.

Use

Luciferase

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLMB708

Plasmid

#83782

Purpose

Tartrate biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

Region upstream RL0996 containing all of RL0997

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLMB710

Plasmid

#83783

Purpose

Formate biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

Region upstream RL4393 (fdsG) containing all of RL4394

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLMB712

Plasmid

#83784

Purpose

Hesperetin biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

Region upstream pRL100185 (nodA) containing part, but not all of, pRL100184 (nodD)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLMB714

Plasmid

#83786

Purpose

C4-dicarboxylates biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

Region upstream RL3424 (dctA)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLMB705

Plasmid

#83787

Purpose

Mannitol biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

Region upstream RL4218 (mtlB) containing all of RL4219

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLMB717

Plasmid

#83788

Purpose

Erythritol biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

Region upstream pRL90085 containing all of pRL90087

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLMB737

Plasmid

#83790

Purpose

Malonate biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

Region upstream RL0990 (matA) containing all of RL0989 (matR)

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLMB738

Plasmid

#83791

Purpose

GABA biosensor plasmid

Depositor

Philip Poole

Article

Pini et al Plant Physiol. 2017 Jul;174(3):1289-1306

Insert

Region upstream RL0102 (gabT) containing all of RL0103

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CBX1wt-PA-mCherry

Plasmid

#138252

Purpose

Wild-type CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry).

Depositor

Jin Zhang

Article

Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

Insert

CBX1-PAmCherry

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

July 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBbdCas9G_arr2

Plasmid

#149571

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9G(RBSmut)_arr2

Plasmid

#149578

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Clover-CytERM-N-17

Plasmid

#56301

Purpose

Localization: Endoplasmic Reticulum, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

CytERM

Tags

Clover

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pNL1 Ecarin

Plasmid

#106289

Purpose

Expresses Ecarin in Ecoli off a T7 promoter in the NEB control cloning vector

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

Ecarin

Expression

Bacterial

Promoter

T7

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9K(-10TC)_arr2

Plasmid

#149584

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9K_arr2

Plasmid

#149570

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9K(RBSmut)_arr2

Plasmid

#149577

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Cas-Acr (D14A/G38A)

Plasmid

#128118

Purpose

Expresses SpyCas9 fused to attenuated AcrIIA4 (D14A/G38A)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (D14A/G38A)

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Park2_L (TAL 3012)

Plasmid

#36004

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting Park2 (prkn Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
Park2_R (TAL 3013)

Plasmid

#36005

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

TAL array targeting Park2 (prkn Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Available Since

April 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
p3E-rab6bb

Plasmid

#109722

Purpose

Multisite gateway vector for 3' tagging with rab6bb

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab6bb (rab6bb Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mNG-mCh

Plasmid

#124215

Purpose

Cytoplasmic mNeongreen mCherry tandem, ~19 % energy transfer pNM002 - pSAV057-mNG-mCh

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al MBio. 2017 Sep 12;8(5). pii: mBio.01089-

Insert

mNG-mCh

Expression

Bacterial

Available Since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2-C11orf83-V5

Plasmid

#65843

Purpose

Mammalian expression of C terminally V5-tagged C11orf83/UQCC3

Depositor

Amos Bairoch

Article

Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

Insert

UQCC3 WT (UQCC3 Human)

Tags

V5

Expression

Mammalian

Mutation

WT

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Myc-CICf_L40S

Plasmid

#48187

Purpose

Expresses L40S mutated Cic

Depositor

Huda Zoghbi

Article

Kim et al Genes Dev. 2013 Mar 15;27(6):590-5. doi:

Insert

Capicua (Cic Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
SI6-IGSF21a

Plasmid

#36141

Depositor

Gavin Wright

Article

Crosnier et al BMC Biol. 2010 Jun 4;8:76. doi: 10.1186/

Insert

IGSF21a mouse monoclonal antibody (igsf21a Zebrafish)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2012

Availability

Academic Institutions and Nonprofits only
EKAR2G_design1_mTFP_wt_Venus_173

Plasmid

#39815

Depositor

Olivier Pertz

Article

Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

Insert

Erk activity reporter

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pFGL1252_TetGFP(Hyg)

Plasmid

#118993

Purpose

TetOFF controlled GFP-tagging vector with Hygromycin selection

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Insert

eGFP

Use

Fungal expression (in magnaporthe oryzae)

Promoter

Ustilago maydis Mfa1 basal promoter with six tetO…

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mGDF8 donor mCherry-PA

Plasmid

#72596

Purpose

Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

mCherry-puromycin cassetter

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only
pDONR_P4-P1R_5'arm_apobb.1

Plasmid

#128662

Purpose

apobb.1 homology arm 5' gateway clone

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

Genomic sequence upstream of apobb.1 stop codon (apobb.1 Zebrafish)

Use

Multi-step gateway cloning

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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