We narrowed to 51,040 results for: des.1

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TET-pLKO.1 PURO shLacZ

Plasmid

#98632

Purpose

Lentiviral shRNA vector for inducible knockdown of LacZ (control hairpin)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shLacZ

Use

Lentiviral

Expression

Mammalian

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1 EB1 shRNA #3

Plasmid

#37927

Depositor

Torsten Wittmann

Article

Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

Insert

EB1 shRNA #3 (MAPRE1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

human U6

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

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Integrin alpha5(1-623)-AHCys

Plasmid

#27301

Depositor

Timothy Springer

Article

Takagi et al EMBO J. 2003 Sep 15. 22(18):4607-15.

Insert

integrin alpha 5 (ITGA5 Human)

Expression

Mammalian

Mutation

alpha 5(1-623)+GG+TEV+GGEN+AHCys

Available Since

March 8, 2011

Availability

Academic Institutions and Nonprofits only
pCA528-AMOT(1-300)-sortase

Plasmid

#166706

Purpose

expresses AMOT(1-300) with C-terminal sortase tag in bacterial cells

Depositor

Wesley Sundquist

Article

Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

Insert

AMOT aa 1-300

Tags

His-SUMO and Sortase tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRSETC-PITPbeta (splice variant 1)

Plasmid

#58270

Purpose

bacterial expression of PITP beta (phosphatidylinositol transfer protein beta)

Depositor

Shamshad Cockcroft

Article

Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

Insert

PITP beta (PITPNB Human)

Tags

HIS

Expression

Bacterial

Promoter

T7

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
pRK-flag-USP19 1-493

Plasmid

#78581

Purpose

Expression flag-USP19 1-493 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 1-493 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

mutated 494 residue to stop codon

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
GLN, Linear-3, PolyU(1)

Plasmid

#166984

Purpose

Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a GLN tRNA promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination module:PolyU(1)

Expression

Mammalian

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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H6-epsin 1 (144-575)

Plasmid

#63008

Purpose

expresses the adaptor protein epsin that lacks its ENTH-domain in bacteria

Depositor

Ernst Ungewickell

Article

Kalthoff et al J Biol Chem. 2002 Mar 8;277(10):8209-16.

Insert

Epsin 1 without ENTH domain (Epn1 Rat)

Tags

His tag

Expression

Bacterial

Available Since

March 24, 2015

Availability

Academic Institutions and Nonprofits only
821 pGEX2T PTEN 1-274

Plasmid

#10741

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Bacterial

Mutation

Amino acids 1-274.

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA FLAG TORC1 (1-630)

Plasmid

#22974

Depositor

Marc Montminy

Article

Conkright et al Mol Cell. 2003 Aug . 12(2):413-23.

Insert

TORC1 (1-630) (CRTC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Amino acids 1-630 rather than 1-634.

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG/MKRN1(1-369)

Plasmid

#78753

Purpose

To overexpress MKRN1(1-369) in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

1-369

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pmiReport PU.1 3' UTR

Plasmid

#26530

Depositor

David Baltimore

Article

O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

Insert

PU.1 3' UTR (Spi1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

Oct. 28, 2010

Availability

Academic Institutions and Nonprofits only
pGEX-5X-1-DJ1-D149A

Plasmid

#29351

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Tags

GST

Expression

Bacterial

Mutation

Asp149Ala

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pCGN-ELK-1 R mut

Plasmid

#27159

Purpose

For mammalian expression of human Elk-1 with an HA tag and a mutation in the R repression domain

Depositor

Ron Prywes

Article

Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

Insert

ELK1 (ELK1 Human)

Tags

HA

Expression

Mammalian

Mutation

R box mutant: K249A/V250A/E251A

Available Since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only
pGEX-5X-1-DJ1-M26I

Plasmid

#29353

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Tags

GST

Expression

Bacterial

Mutation

Met26Ile

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
p3x-Flag Gli3 30R3-1

Plasmid

#120304

Purpose

Expresses human protein Gli containing a 4HT-inducible intein evolved to operate at 30C

Depositor

David Liu

Article

Peck et al Chem Biol. 2011 May 27;18(5):619-30. doi

Insert

30R3-1, Gli3

Use

Synthetic Biology

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-mMcl-1 KR

Plasmid

#32981

Depositor

Joseph Opferman

Article

Stewart et al Mol Cell Biol. 2010 Jun;30(12):3099-110.

Insert

Mcl-1 (Mcl1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

All Lys to Arg

Promoter

LTR

Available Since

March 15, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-5X-1-DJ1-E64D

Plasmid

#29352

Depositor

Mark Cookson

Article

Blackinton et al Brain Res Mol Brain Res. 2005 Mar 24. 13

Insert

DJ1 (PARK7 Human)

Tags

GST

Expression

Bacterial

Mutation

Glu64Asp

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pRK5-HA-SZT2 1-1021

Plasmid

#87037

Purpose

mammalian expression of SZT2 fragment

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Tags

HA

Expression

Mammalian

Mutation

AA 1-1021

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-2a-1

Plasmid

#22764

Depositor

Elisa Izaurralde

Article

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 2a-1 (mir-2a-1 Fly, primary transcript)

Use

Drosophila expression

Available Since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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