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We narrowed to 7,566 results for: ef1a

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Showing: 7301 - 7350 of 7566 results

  9. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

    Plasmid
    #239448
    Purpose
    Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.
    Depositor
    Alexis Komor
    Inserts
    S.p. gRNA backbone
    S.a. gRNA backbone
    PuroR-T2A-BFP(C>T_screening)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    BFP: T65S, S72S, V145F, K206K, H231L
    Promoter
    EF1alpha, U6, and mU6
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239448-plasmid-map-sequence-id-476590

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  13. BII-gR-PnGW

    Plasmid
    #133355
    Purpose
    Piggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications), co-expressed with GFP-NLS
    Depositor
    Blair Madison
    Article
    Fischer et al Am J Physiol Gastrointest Liver Physiol.
    Insert
    GFP-NLS
    Tags
    HA-tagged GFP
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available Since
    Jan. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    133355-plasmid-map-sequence-id-267392

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  22. Fireworks RFP[PTC-] (AVA2515)

    Plasmid
    #85443
    Purpose
    Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.
    Depositor
    Joan Steitz
    Article
    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:
    Insert
    tDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-HygromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Expression
    Mammalian
    Available Since
    Feb. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85443-plasmid-map-sequence-id-171526

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  30. p1.1-eGFP

    Plasmid
    #162738
    Purpose
    Fluorescent reporter for CHO cells expression studies
    Depositor
    Ivan Vorobiev
    Article
    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:
    Insert
    enhanced green fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    July 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    162738-plasmid-map-sequence-id-337724

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  32. pMpGWB321

    Plasmid
    #68649
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Promoter
    MpEF1alpha promoter
    Available Since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68649-plasmid-map-sequence-id-121824

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  33. pMpGWB121

    Plasmid
    #68575
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Promoter
    MpEF1alpha promoter
    Available Since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68575-plasmid-map-sequence-id-121822

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  34. p1.2-Zeo-eGFP

    Plasmid
    #162743
    Purpose
    Fluorescent reporter for CHO cells expression studies
    Depositor
    Ivan Vorobiev
    Article
    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:
    Insert
    enhanced green fluorescent protein
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available Since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162743-plasmid-map-sequence-id-339096

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  35. pMpGWB118

    Plasmid
    #68572
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Promoter
    MpEF1alpha promoter
    Available Since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68572-plasmid-map-sequence-id-121810

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  36. pMpGWB218

    Plasmid
    #68609
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Promoter
    MpEF1alpha promoter
    Available Since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68609-plasmid-map-sequence-id-121811

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  37. pMpGWB221

    Plasmid
    #68612
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Promoter
    MpEF1alpha promoter
    Available Since
    July 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    68612-plasmid-map-sequence-id-121823

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  43. pMpGWB418

    Plasmid
    #68683
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Promoter
    MpEF1alpha promoter
    Available Since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68683-plasmid-map-sequence-id-121813

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  44. pMpGWB421

    Plasmid
    #68686
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Promoter
    MpEF1alpha promoter
    Available Since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68686-plasmid-map-sequence-id-121825

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  46. Fireworks GFP[PTC-] (AVA2598)

    Plasmid
    #85445
    Purpose
    Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.
    Depositor
    Joan Steitz
    Article
    Alexandrov et al Mol Cell. 2017 Jan 5;65(1):191-201. doi:
    Insert
    tDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
    Use
    Minimal backbone fragment for low-copy bacterial …
    Expression
    Mammalian
    Available Since
    Feb. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    85445-plasmid-map-sequence-id-171491

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Showing: 7301 - 7350 of 7566 results