We narrowed to 14,031 results for: TIM

1. PE-P3-maize

   Plasmid

   #185611

   Purpose

   For plant prime editing in maize plants

   Depositor

   Jinxiao Yang

   Article

   Xu et al Nat Plants. 2022 Jan;8(1):45-52. doi: 10

   Insert

   3XFLAG-4XNLS-M-MLV-nCas9(H840A)-4XNLS
   

   Use

   CRISPR

   Promoter

   ZmUbi

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCytERM-Electra1

   Plasmid

   #179476

   Purpose

   ER Visualization (OSER assay) Expresses Electra1 in fusion with N-terminal part of p450

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   CytERM-Electra1
   

   Tags

   Electra1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMX Vav1 uGFP

   Plasmid

   #14557

   Depositor

   Joan Brugge

   Article

   Wilsbacher et al Cell Commun Signal. 2006 May 18;4:5. doi

   Insert

   Vav1 (VAV1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   April 12, 2007

   Availability

   Academic Institutions and Nonprofits only

4. NLS-GI-VP16-IRES-NLS-GAL4DBD-FKBP-SMBiT-FKF1

   Plasmid

   #183402

   Purpose

   Dual expression of GI-VP16 and GAL4DBD-FKBP-SmBiT-FKF1 in the nucleus

   Depositor

   Alice Ting

   Article

   Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

   Insert

   GI-VP16-IRES-GAL4DBD-FKBP-SMBiT-FKF1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. LAMP-OeNL_pcDNA3

   Plasmid

   #89525

   Purpose

   Orange color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   OeNL
   

   Tags

   LAMP1

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-natMX6-Purg1

   Plasmid

   #39350

   Depositor

   Jurg Bahler

   Article

   Watt et al PLoS One. 2008 Jan 16;3(1):e1428.

   Insert

   urg1 promoter (urg1, urg1 Fission Yeast)
   

   Use

   Yeast genomic targeting

   Tags

   natMX6 module - reverse orientation

   Available Since

   Nov. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pCAG-eGtACR1-mRuby2-ST

   Plasmid

   #109136

   Purpose

   Anion channelrhodopsin GtACR1 fused to ER export signal, mRuby2 and targeted to the neuronal soma and proximal dendrites

   Depositor

   Hillel Adesnik

   Article

   Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

   Insert

   eGtACR1-mRuby2-ST
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. mNG-sfTq2

   Plasmid

   #124218

   Purpose

   Cytoplasmic mNeongreen superfolder mTurquoise2 tandem, ~60 % energy transfer. pNM044 - pSAV057-mNG-sfTq2

   Depositor

   Tanneke den Blaauwen

   Article

   Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

   Insert

   mNG-sfTq2
   

   Expression

   Bacterial

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMET7-GAG-HRAS

   Plasmid

   #80604

   Purpose

   Mammalian expression vector that expresses the HIV-1 GAG protein with HRAS fused to it

   Depositor

   Sven Eyckerman

   Article

   Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

   Insert

   HIV-1 p55 GAG
   

   Expression

   Mammalian

   Promoter

   SRalpha

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. ptagBFP-LacI

    Plasmid

    #103839

    Purpose

    Flourescent marker for lacO arrays

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    tagBFP-LacI
    

    Expression

    Mammalian

    Mutation

    LacI: C19T (silent, L7L), T264C (silent, A88A), C…

    Promoter

    SV2

    Available Since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC1750

    Plasmid

    #127984

    Purpose

    AMA1 plasmid with Aspergillus optimized Cpf1, ble selection marker, and USER cassette (PacI/Nt.BbvCI)

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al Fungal Biol Biotechnol. 2019 May 1;6:6.

    Insert

    Cpf1
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Mutation

    codon optimized for Aspergillus nidulans

    Promoter

    Aspergillus nidulans tef1 promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-PxCas12a-2AeGFP

    Plasmid

    #123637

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

    Insert

    PxCas12a
    

    Tags

    3xHA tag

    Expression

    Mammalian

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEB2-TagRFP

    Plasmid

    #103997

    Purpose

    Plasmid encoding TagRFP

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    TagRFP
    

    Use

    Low copy

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, K42R, K67R, …

    Promoter

    proC

    Available Since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5/FRT/TO_LAMP1_STf

    Plasmid

    #182006

    Purpose

    CMV overexpression of SNAP-tag fast in the lysosome of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    LAMP1_STf
    

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMET7-GAG-GW

    Plasmid

    #194634

    Purpose

    Gateway destination vector for mammalian expression of Virotrap bait constructs

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    p55 GAG
    

    Tags

    PQS1

    Expression

    Mammalian

    Promoter

    SRalpha

    Available Since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZR064_CROPseq-sgCD4-CRISPRi

    Plasmid

    #180270

    Purpose

    Lentiviral CROPseq vector expressing CD4 targeting sgRNA for CRISPRi

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Insert

    CD4 sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV MN (eGFP)

    Plasmid

    #89932

    Purpose

    Expresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

    Depositor

    Carl Novina

    Article

    Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

    Insert

    MN
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    M.SssI residues 2-272 fragment

    Promoter

    CMV

    Available Since

    Sept. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. H2A-mTurquoise-RHOG-QL-deltaCAAX

    Plasmid

    #176096

    Purpose

    Constitutively active, nuclear localized RhoG

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

    Insert

    RHOG (RHOG Human)
    

    Expression

    Mammalian

    Mutation

    QL

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. KA1158_pPBCAG-MerCreMer-IZ

    Plasmid

    #124184

    Purpose

    piggyBac-based expression of the tamoxifen-inducible Cre

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Insert

    MerCreMer-IRES-Zeo
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUCBB-ntH6-eGFP

    Plasmid

    #32557

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    6 His tag with Thrombin site

    Expression

    Bacterial

    Promoter

    Consitutive lac

    Available Since

    Oct. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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