We narrowed to 9,829 results for: pho;

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pcDNA5/FRT/TO-Flag-Tipin-L195A

Plasmid

#23118

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Tipin (TIPIN Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed Leucine 195 to Alanine

Available Since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 L555A

Plasmid

#19851

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 L555A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 555 to Alanine

Available Since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only
pET28aAviHisCD72aCTLD

Plasmid

#129065

Purpose

generation of biotinylated mouse CD72a CTLD protein using bacteria

Depositor

Takeshi Tsubata

Article

Akatsu et al J Exp Med. 2016 Nov 14;213(12):2691-2706

Insert

CD72a (Cd72 Mouse)

Expression

Bacterial

Availability

Academic Institutions and Nonprofits only

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pEGFP-2xFYVE

Plasmid

#140047

Purpose

Expresses GFP-tagged HRS 2xFYVE in mammalian cells

Depositor

Harald Stenmark

Article

Gillooly et al EMBO J. 2000 Sep 1;19(17):4577-88. doi:

Insert

tandem HRS FYVE domain (Hgs Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

two FYVE domains (amino acids 147-223), linked by…

Promoter

CMV

Available Since

May 3, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Flag-His-ATM wt

Plasmid

#31985

Purpose

Mammalian expression of human ATM with Flag and His tags

Depositor

Michael Kastan

Article

Canman et al Science. 1998 Sep 11;281(5383):1677-9.

Insert

ATM wild-type (ATM Human)

Tags

Flag and HIS

Expression

Mammalian

Mutation

3' UTR of 400 nucl.

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pJ4M/TDP-43

Plasmid

#104480

Purpose

bacterial expression of TDP-43

Depositor

Nicolas Fawzi

Article

Wang et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

Insert

TDP-43 (TARDBP Human)

Tags

MBP-6xHis

Expression

Bacterial

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAC4 Frk-SH2

Plasmid

#46428

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Frk (FRK Human)

Tags

AviTag, GST, and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 108-213

Promoter

Trc

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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mouse E-cadherin GFP (423)

Plasmid

#67937

Purpose

Mammalian expression of E-cadherin-GFP

Depositor

Alpha Yap

Article

Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

Insert

E-cadherin (Cdh1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pT7-7 asyn E46K

Plasmid

#105728

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Article

Mbefo et al J Biol Chem. 2015 Apr 10;290(15):9412-27

Insert

alpha-synuclein E46K (SNCA Human)

Expression

Bacterial

Mutation

E46K

Promoter

T7

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Flag-His-ATM kd

Plasmid

#31986

Depositor

Michael Kastan

Article

Canman et al Science. 1998 Sep 11;281(5383):1677-9.

Insert

ATM kd (ATM Human)

Tags

Flag and His

Expression

Mammalian

Mutation

3'UTRf 9 nucl/D2870A N2875k. catalytically …

Available Since

March 12, 2012

Availability

Academic Institutions and Nonprofits only

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pLEX307_PDHA2S293D

Plasmid

#115194

Purpose

Lentiviral transduction and expression of PDHA2S293D into any mammalian cell

Depositor

Mohan Babu

Article

Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

Insert

PDHA2S293D (PDHA2 Human)

Use

Lentiviral

Mutation

p.S293D

Promoter

EF1alpha

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-dCas9-KRAB-PGK-HygR

Plasmid

#83890

Purpose

Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

humanized dead Cas9 KRAB
aminoglycoside phosphotransferase from E. coli

Use

CRISPR and Lentiviral

Tags

Flag

Mutation

D10A and H840A

Promoter

Human Ubiquitin C Promoter and mouse phosphoglyce…

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-EEA1 wt

Plasmid

#42307

Depositor

Silvia Corvera

Article

Lawe et al J Biol Chem. 2000 Feb 4;275(5):3699-705.

Insert

EEA1 (EEA1 Human)

Tags

GFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pLEGFP-WT-STAT3

Plasmid

#71450

Purpose

Retroviral expression of WT-STAT3. Please note that this plasmid contains WT-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX Slp76-SH2

Plasmid

#46503

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Slp76 (LCP2 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 417-533

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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GFP-MAP1B

Plasmid

#44396

Depositor

Phillip Gordon-Weeks

Article

Scales et al J Cell Sci. 2009 Jul 15;122(Pt 14):2424-

Insert

MAP1B (Map1b Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Myc-His beta4

Plasmid

#16039

Depositor

Filippo Giancotti

Article

Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

Insert

beta4 (ITGB4 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pHTN-LC3B

Plasmid

#219440

Purpose

Expresses HaloTag-LC3B, an autophagosome marker

Depositor

Erika Holzbaur

Article

Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

Insert

LC3B (Map1lc3b Rat)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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H2B-GFP-Aurora B WT

Plasmid

#184042

Purpose

Expresses H2B-GFP fused to Aurora B WT

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Insert

AURKB (AURKB Human)

Tags

EGFP and H2B

Expression

Mammalian

Promoter

CMV

Available Since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-Drosha

Plasmid

#62520

Purpose

wild-type human Drosha cDNA is subcloned into pEGFP-C1 vector between HindIII and KpnI sites

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

Insert

human Drosha full length (DROSHA Human)

Tags

GFP

Expression

Mammalian

Available Since

March 20, 2015

Availability

Academic Institutions and Nonprofits only

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