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pME-p3E-HyPer7

Plasmid

#194289

Purpose

Multisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQV

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

Hyper7

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII

Plasmid

#174424

Purpose

Expresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteria

Depositor

Thomas Pucadyil

Article

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1

Use

Tags

6xHis(Thrombin)EGFP(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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FUGW-eGFP-ZEB1 3'UTR

Plasmid

#81018

Purpose

FUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscript

Depositor

Jeffrey Rosen

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

Zeb1 3'UTR (UTS2R Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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AviTag-SpyTag003-MBP

Plasmid

#184227

Purpose

Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

Depositor

Mark Howarth

Article

Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

Insert

AviTag-SpyTag003-MBP

Use

Tags

AviTag, His6, SpyTag003, and Thrombin cleavage si…

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCW57.1-DUX4-CA

Plasmid

#99281

Purpose

All-in-one lentivector with tet-inducible DUX4 (codon sequences altered)

Depositor

Stephen Tapscott

Article

Jagannathan et al Hum Mol Genet. 2016 Oct 15;25(20):4419-4

Insert

DUX4 (codon altered) (DUX4 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

codon altered

Promoter

TRE

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pJS646

Plasmid

#183175

Purpose

Y2H vector compatible with pJS647 PYR1 insert

Depositor

Timothy Whitehead

Article

Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pClneoMyc human NDR1

Plasmid

#37023

Depositor

Yutaka Hata

Article

Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

Insert

NDR1 (STK38 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 23, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag4A-MITF-M (wt)

Plasmid

#31151

Depositor

Yardena Samuels

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

MITF-M (MITF Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

WT

Promoter

Available since

July 22, 2011

Availability

Academic Institutions and Nonprofits only

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pLV.ATMi

Plasmid

#14542

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

Insert

ATM shRNA (ATM Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2007

Availability

Academic Institutions and Nonprofits only

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DYSF-Venus

Plasmid

#29768

Depositor

Steven Vogel

Article

Covian-Nares et al J Cell Sci. 2010 Jun 1. 123(Pt 11):1884-

Insert

DYSF-Venus (DYSF Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
pBbdCas9S(RBSmut)_Psyn-sgRNA500

Plasmid

#149576

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PflaB, PpQE30, Psyn

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-RFP657

Plasmid

#75162

Purpose

Derived from LentiCRISPR v1 but expresses RFP657 instead of puromycin resistance

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

RFP657

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLXSN-MRGPRX2

Plasmid

#213960

Purpose

Expresses MRGPRX2 in mammalian cells

Depositor

Christa Müller

Article

Al Hamwi et al Commun Biol. 2024 Jan 6;7(1):52. doi: 10

Insert

MRGPRX2 (MRGPRX2 )

Use

Retroviral

Tags

Expression

Mutation

Promoter

LTR

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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15x-QUAS-dTomato-T2A-TRPV1

Plasmid

#140945

Purpose

QUAS reporter line that expresses cytosolic dTomato and the rat TRPV1 ion channel, separated by T2A

Depositor

Leslie Vosshall

Article

Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

Insert

15x-QUAS-dTomato-T2A-TRPV1

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_nLightG

Plasmid

#217656

Purpose

Expresses norepinephrine indicator nLightG under mammalian promoter

Depositor

Tommaso Patriarchi

Article

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Insert

nLightG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pWZL N-Myc-hPOT1_deltaOB

Plasmid

#166408

Purpose

express hPOT1 ΔOB in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

POT1 ΔOB (POT1 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

ΔOB

Promoter

Available since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMH-Cas9-gate

Plasmid

#113742

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR; Gateway destination vector

Tags

Expression

Mutation

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pLV.ATRi

Plasmid

#14543

Depositor

Didier Trono

Article

Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

Insert

ATR shRNA (ATR Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2007

Availability

Academic Institutions and Nonprofits only

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pDragon-empty

Plasmid

#155018

Purpose

Empty Tigre-Dragon vector. Genes of interest can be cloned using Mlu-I and SnaB-I. For Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA.

Depositor

Alberto Rosello-Diez

Article

Ahmadzadeh et al Development. 2020 May 28;147(10). pii: d

Type

Empty backbone

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-eCas9-sgNT

Plasmid

#140238

Purpose

Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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