4,204 results

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pCDNA3 Flag p38 alpha

Plasmid

#20351

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 alpha (Mapk14 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-HA (New MCS)

Plasmid

#32530

Depositor

Christopher A Walsh

Type

Empty backbone

Tags

HA

Expression

Mammalian

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pCVL SFFV d14GFP EF1s HA.NLS.Sce(opt)

Plasmid

#31476

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d14 GFP donor EF1s I-SceI

Use

Lentiviral

Tags

HA and NLS

Mutation

GFP has 14 amino acids deleted from c terminus

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pSV Sport PPAR gamma 2

Plasmid

#8862

Depositor

Bruce Spiegelman

Article

Tontonoz et al Genes Dev. 1994 May 15. 8(10):1224-34.

Insert

PPAR gamma (Pparg Mouse)

Expression

Mammalian

Available Since

Feb. 17, 2006

Availability

Academic Institutions and Nonprofits only

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SepOTSλ

Plasmid

#68292

Purpose

Phosphoserine orthogonal translation system (preferred variant)

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS9/EFSep21, 4xtRNA-Sep-A37

Use

Synthetic Biology

Expression

Bacterial

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-hsp27 wt FL

Plasmid

#17444

Depositor

Andrea Doseff

Article

Voss et al J Biol Chem. 2007 Aug 24. 282(34):25088-

Insert

small heat shock protein27 (HSPB1 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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CHOP promoter (-649/+136) pmCherry-1

Plasmid

#36035

Depositor

Quan Lu

Article

Oh et al J Biol Chem. 2012 Feb 17;287(8):6025-34.

Insert

CHOP promoter (-647 to +136) (DDIT3 Human)

Expression

Mammalian

Available Since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pSin-EF2-Sox2-Pur

Plasmid

#16577

Depositor

James Thomson

Article

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens SRY (sex determining region Y)-box 2 (SOX2) (SOX2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pTDH3-dCas9

Plasmid

#46920

Purpose

Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS controlled by TDH3 promoter

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9

Use

CRISPR

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pMSP1E3

Plasmid

#20064

Depositor

Stephen Sligar

Article

Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

Insert

MSP1E3 (APOA1 synthetic gene)

Tags

6-His

Expression

Bacterial

Mutation

"extended" MSP1; contains repeats of he…

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-JMJD2B

Plasmid

#24181

Purpose

Mammalian expression of human JMJD2B with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

JMJD2B (KDM4B Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-MEK-DD

Plasmid

#15268

Depositor

William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

Map2k1 (Mapk1 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

S218D S222D

Available Since

July 12, 2007

Availability

Academic Institutions and Nonprofits only

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pMH0001

Plasmid

#85969

Purpose

UCOE-SFFV-dCas9-BFP-KRAB

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Insert

dCas9

Use

Lentiviral

Tags

BFP-KRAB

Expression

Mammalian

Mutation

D10A, H840A

Promoter

SFFV

Available Since

Feb. 3, 2017

Availability

Academic Institutions and Nonprofits only

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Tailless VinculinTS

Plasmid

#26020

Depositor

Martin Schwartz

Article

Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

Insert

tailless vinculinTS

Expression

Mammalian

Available Since

Sept. 17, 2010

Availability

Academic Institutions and Nonprofits only

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pUC19-HRP-C

Plasmid

#40163

Depositor

Paul Ortiz de Montellano

Article

Hartmann et al Arch Biochem Biophys. 1992 Aug 15;297(1)

Insert

Horseradish peroxidase

Use

Coli cloning vector

Tags

Six histidine

Available Since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

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pCI-SEP GluR2(R)

Plasmid

#24001

Depositor

Robert Malinow

Article

Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

Insert

GluR2 (Gria2 Rat)

Tags

GluR1 signal peptide (aa1-22) and SEP (supereclip…

Expression

Mammalian

Promoter

CMV

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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-1748 human cyclin D1 promoter pGL3Basic

Plasmid

#32726

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase; Reporter gene

Promoter

-1748CCND1 promoter

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42229

Purpose

This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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Klotho (membrane)

Plasmid

#17712

Depositor

Hal Dietz

Article

Arking et al Proc Natl Acad Sci U S A. 2002 Jan 22. 9

Insert

Klotho (KL Human)

Tags

V5

Expression

Mammalian

Mutation

P101S

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pMK286 (mAID-NeoR)

Plasmid

#72824

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Neo

Use

CRISPR

Tags

mAID

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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