4,139 results

1. AAV-ReaChR-citrine

   Plasmid

   #50954

   Purpose

   ReaChR-citrine in AAV2 vector under human synapsin promoter

   Depositor

   Roger Tsien

   Article

   Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

   Insert

   ReaChR-citrine
   

   Use

   AAV

   Tags

   citrine

   Expression

   Mutation

   Promoter

   truncated human Synapsin promoter

   Available since

   Feb. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSaCas9_GFP

   Plasmid

   #64709

   Purpose

   Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells

   Depositor

   Kiran Musunuru

   Insert

   SaCas9-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP and 3xFLAG-SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lentiviral-FOP-dGFP-reporter

   Plasmid

   #14885

   Depositor

   Tannishtha Reya

   Article

   Reya et al Nature. 2003 May 22. 423(6938):409-14.

   Insert

   mutant LEF-1/TCF-responsive promoter (Ctnnb1 )
   

   Use

   Lentiviral

   Tags

   d2 eGFP

   Expression

   Mammalian

   Mutation

   mutant TCF/LEF binding sites

   Promoter

   Available since

   Aug. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLYS1-FLAG-MitoGFP-HA

   Plasmid

   #50057

   Purpose

   Expression of GFP targeted to mitochondria with Flag and HA tags

   Depositor

   Vamsi Mootha

   Article

   Sancak et al Science. 2013 Nov 14.

   Insert

   Mito-GFP
   

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. G-CaMP3

   Plasmid

   #22692

   Purpose

   a single-wavelength GCaMP2-based genetically encoded calcium indicator

   Depositor

   Loren Looger

   Article

   Tian et al Nat Methods. 2009 Dec;6(12):875-81.

   Insert

   G-CaMP3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N363D mutation (increased fluorescence)

   Promoter

   Available since

   Nov. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. p212 pCMV-EGFP/RFP

   Plasmid

   #8389

   Purpose

   Gene-switch Cre reporter plasmid with floxed EGFP gene upstream of RFP.

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   floxed EGFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 31, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. G6PD/pRK5

   Plasmid

   #41521

   Depositor

   Xiaolu Yang

   Article

   Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

   Insert

   glucose-6-phosphate dehydrogenase (G6PD Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ac5-STABLE2-neo

   Plasmid

   #32426

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Insert

   Flag-mCherry-T2A-GFP-T2A-neo
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-HDAC4

   Plasmid

   #45636

   Depositor

   Tso-Pang Yao

   Article

   Cohen et al J Biol Chem. 2007 Nov 16;282(46):33752-9

   Insert

   HDAC4 (HDAC4 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-Cre-GFP

    Plasmid

    #68544

    Purpose

    AAV expression of Cre-GFP fusion

    Depositor

    Eric Nestler

    Article

    Kennedy et al Nat Neurosci. 2013 Apr;16(4):434-40. doi

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. TetO-FUW-NICD

    Plasmid

    #61540

    Purpose

    Expresses NOTCH-ICD in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    NOTCH-ICD (NOTCH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    NOTCH intracellular domain (aa1761-2555); A1943S…

    Promoter

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTRK669

    Plasmid

    #71313

    Purpose

    Helper plasmid for pORI plasmids. Chloramphenicol resistant, temperature sensitive replication in E. coli and gram positives. Encodes RepA.

    Depositor

    Todd Klaenhammer, Rodolphe Barrangou

    Article

    Russell et al Appl Environ Microbiol. 2001 Sep;67(9):4

    Type

    Empty backbone

    Use

    E. coli-gram positive bacteria shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBA439

    Plasmid

    #85967

    Purpose

    Perturb-seq vector backbone

    Depositor

    Jonathan Weissman

    Article

    Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

    Inserts

    sgGFP-NT2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. mTagBFP2-TOMM20-N-10

    Plasmid

    #55328

    Purpose

    Localization: Outer Mitochondrial Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    TOMM20 (TOMM20 Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. mApple-N1

    Plasmid

    #54567

    Purpose

    Localization: N1 Cloning Vector, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Article

    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

    Type

    Empty backbone

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV5 TBRI-His

    Plasmid

    #19161

    Depositor

    Joan Massague

    Insert

    transforming growth factor, beta receptor 1 (TGFBR1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-myc3-CUL1

    Plasmid

    #19896

    Depositor

    Yue Xiong

    Article

    Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

    Insert

    cullin 1 (CUL1 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLM-mCherry-Klf4

    Plasmid

    #23243

    Depositor

    Michel Sadelain

    Article

    Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

    Insert

    mCherry_2A_KLF4 (KLF4 synthetic fluorescent protein, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC1-REX-YFP

    Plasmid

    #48247

    Purpose

    Genetically encoded fluorescent indicator for measuring NAD/NADH ratio.

    Depositor

    Vsevolod Belousov

    Article

    Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:

    Insert

    REX-YFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pdCas9-humanized

    Plasmid

    #44246

    Purpose

    Expression of a catalytically inactive, human codon-optimized Cas9 under the control of Murine Stem Cell retroVirus LTR promoter for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    dead Cas9 with 3X NLS
    

    Use

    CRISPR and Retroviral; Vector

    Tags

    3x NLS

    Expression

    Mammalian

    Mutation

    D10A H840A (catalytically inactive)

    Promoter

    MSCV LTR promoter

    Available since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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