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pEGFP hsp70 K71E

Plasmid

#15216

Depositor

Lois Greene

Publication

Zeng et al J Cell Sci. 2004 Oct 1. 117(Pt 21):4991-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hsp70 K71E (HSPA1A Human)

Tags

EGFP

Expression

Mammalian

Mutation

K71E, ATPase-deficient mutant.

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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CD20

Plasmid

#1890

Depositor

Sander van den Heuvel

Publication

van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CD20 (MS4A1 Human)

Expression

Mammalian

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMKO.1 puro hTERT shRNA

Plasmid

#10688

Depositor

William Hahn

Publication

Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

Insert

hTERT shRNA (TERT Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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ABE8.20-m

Plasmid

#136300

Purpose

expresses ABE8.20-m in mammalian cells

Depositor

Nicole Gaudelli

Publication

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ABE8.20-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pET51b(+)_EGFP_NbALFA

Plasmid

#136626

Purpose

Plasmid encoding the bacterial expression of NbALFA for ALFA-tag as an EGFP-fusion

Depositor

Kai Johnsson

Publication

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

EGFP-NbALFA

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-TRE-EGFP

Plasmid

#89875

Purpose

EGFP reporter vector of iTango system for AAV production

Depositor

Hyungbae Kwon

Publication

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

tetO-EGFP

Use

AAV

Expression

Mammalian

Promoter

tetO

Available since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2

Plasmid

#74009

Purpose

Tissue-specific knock-out in zebrafish, Cas9 and GFP driven by a UAS promoter

Depositor

Filippo Del Bene

Publication

Di Donato et al Genome Res. 2016 May;26(5):681-92. doi:

Inserts

5x UAS
Cas9
T2A
GFP
U6 promoter

Use

CRISPR

Expression

Zebrafish

Available since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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PMXS-NDI1

Plasmid

#72876

Purpose

expresses NDI1 in mammalian cells

Depositor

David Sabatini

Publication

Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

Insert

NDI1 (NDI1 Budding Yeast)

Use

Retroviral

Expression

Mammalian

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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pCMV-lyso-pHoenix

Plasmid

#70112

Purpose

Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

Depositor

Christian Rosenmund, Benjamin Rost

Publication

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

lyso-pHoenix (Cd63 Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat, Mouse)

Tags

Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

Expression

Mammalian

Promoter

CMV

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pmCherry-C1 mCherry-NLS

Plasmid

#58476

Purpose

Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

Depositor

Dyche Mullins

Publication

Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

3X NLS with 3X stop codons

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pCS2-nCas9n-nanos 3’UTR

Plasmid

#62542

Purpose

expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

Depositor

Antonio Giraldez

Publication

Moreno-Mateos et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

nanos 3' UTR (nanos3 Zebrafish)

Use

CRISPR

Mutation

3' UTR only

Promoter

SP6

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET-dCas9-VP64-6xHis

Plasmid

#62935

Purpose

Expression of dCas9-VP64-6xHis in bacterial cells

Depositor

David Liu

Publication

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

dCas9-VP64

Tags

3xFLAG, 6xHis, NLS, and VP64

Expression

Bacterial

Mutation

D10A and H840A amino acid changes render Cas9 nuc…

Promoter

T7

Available since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

Plasmid

#62723

Purpose

AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.

Depositor

Edward Boyden

Publication

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Has service

AAV1 and AAV5

Insert

ChrimsonR-tdTomato

Use

AAV

Tags

tdTomato

Expression

Mammalian

Mutation

Chrimson K176R mutant

Promoter

human synapsin promoter

Available since

April 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

71 Articles

1 Data Hub Report

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mEmerald-Mito-7

Plasmid

#54160

Purpose

Localization: Mitochondria, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Publication

Planchon et al Nat Methods. 2011 May;8(5):417-23. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mito (COX8A Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

17 Articles

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DsRed2-N1

Plasmid

#54493

Purpose

Localization: N1 Cloning Vector, Excitation: 558, Emission: 583

Depositor

Michael Davidson

Type

Empty backbone

Tags

DsRed2

Expression

Mammalian

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

8 Articles

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DRH334: FCK-QuasAr2-mO2

Plasmid

#51692

Purpose

Fluorescent reporter of membrane voltage with improved sensitivity

Depositor

Adam Cohen

Publication

Hochbaum et al Nat Methods. 2014 Aug;11(8):825-33. doi:

Insert

QuasAr2-mOrange

Use

Lentiviral

Tags

mOrange2

Expression

Mammalian

Promoter

CaMKIIa

Available since

April 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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AAV-ReaChR-citrine

Plasmid

#50954

Purpose

ReaChR-citrine in AAV2 vector under human synapsin promoter

Depositor

Roger Tsien

Publication

Lin et al Nat Neurosci. 2013 Oct;16(10):1499-508.

Insert

ReaChR-citrine

Use

AAV

Tags

citrine

Promoter

truncated human Synapsin promoter

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pGFP-Cytochrome C

Plasmid

#41181

Depositor

Douglas Green

Publication

Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cytochrome C (CYCS Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pEGFP-hsp27 wt FL

Plasmid

#17444

Depositor

Andrea Doseff

Publication

Voss et al J Biol Chem. 2007 Aug 24. 282(34):25088-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

small heat shock protein27 (HSPB1 Human)

Tags

GFP

Expression

Mammalian

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pFA6a-link-yomRuby2-SpHis5

Plasmid

#44858

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Publication

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomRuby2

Expression

Yeast

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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