4,178 results

1. GPD-roGFP

   Plasmid

   #49436

   Purpose

   Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells

   Depositor

   Paul Schumacker

   Article

   Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

   Insert

   GPD-roGFP2
   

   Use

   Adenoviral

   Tags

   Glycerol phosphate dehydrogenase, 5' to roGF…

   Expression

   Mammalian

   Mutation

   C48S, Q80R, S147C, and Q204C in Clontech's G…

   Promoter

   CMV

   Available Since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. xCas9 3.7

   Plasmid

   #108379

   Purpose

   Mammalian expression vector for xCas9 3.7

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9 3.7
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   A262T, R324L, S409I, E480K, E543D, M694I, E1219V

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe bleo human PPAR gamma 2

   Plasmid

   #11439

   Depositor

   Ronald Kahn

   Insert

   PPAR gamma 2 (PPARG Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-Azurite

   Plasmid

   #36086

   Purpose

   3rd generation lentiviral plasmid

   Depositor

   Pantelis Tsoulfas

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-15b-TEV-C9R

   Plasmid

   #118754

   Purpose

   TEV-C9R protease expression vector with enhanced expression, solubility and yield without affecting TEV's protease activity

   Depositor

   Yutaka Kuroda

   Article

   Nautiyal et al N Biotechnol. 2018 May 25;42:77-84. doi:

   Insert

   Tobacco Etch Virus protease (NEWENTRY Synthetic)
   

   Tags

   C9R and His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCerulean N1

   Plasmid

   #27795

   Depositor

   Steven Vogel

   Article

   Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

   Insert

   mCerulean N1
   

   Expression

   Mammalian

   Available Since

   April 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV5B-Flag-Smurf2 wt

   Plasmid

   #11746

   Depositor

   Jeff Wrana

   Article

   Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

   Insert

   SMURF2 (SMURF2 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 12, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pRS300

   Plasmid

   #22846

   Purpose

   Backbone for expressing plant artificial miRNAs

   Depositor

   Detlef Weigel

   Article

   Schwab et al Plant Cell. 2006 May . 18(5):1121-33.

   Insert

   MIR319a (MIR319a Mustard Weed)
   

   Use

   RNAi; Plant gene inactivation

   Expression

   Plant

   Available Since

   Jan. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1/hChR2(H134R)-mCherry

   Plasmid

   #20938

   Purpose

   Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Expression

   Mammalian

   Mutation

   H134R mutation in ChR2

   Promoter

   CMV

   Available Since

   June 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-ACE2-GFP

    Plasmid

    #154962

    Purpose

    pcDNA3.1-ACE2-GFP

    Depositor

    Utpal Pajvani

    Article

    Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

    Insert

    ACE2-GFP (ACE2 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    July 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 Flag C/EBPa

    Plasmid

    #66978

    Purpose

    Expression plasmid for mouse C/EBPa; adds Flag tag to the N-terminus of protein

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    C/EBP alpha (Cebpa Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-Clover-mRuby2

    Plasmid

    #49089

    Purpose

    Clover-mRuby2 tandem fusion for FRET calibration

    Depositor

    Kurt Beam

    Insert

    Clover-mRuby2 fusion
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

    Plasmid

    #103870

    Purpose

    High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GSGGGGS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Available Since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1-FLAG-Ku70

    Plasmid

    #46957

    Purpose

    Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    Ku70 (XRCC6 Human)
    

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. SIRT1 Flag

    Plasmid

    #13812

    Purpose

    Mammalian expression of human SIRT1 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT1 (SIRT1 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Mutation

    N-terminus truncated

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-Flag-hsDicer

    Plasmid

    #41584

    Depositor

    Phil Sharp

    Article

    Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

    Insert

    hsDicer (DICER1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available Since

    Jan. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas9D10A_GFP

    Plasmid

    #44720

    Purpose

    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells

    Depositor

    Kiran Musunuru

    Article

    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

    Insert

    Cas9D10A-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP

    Expression

    Mammalian

    Mutation

    D10A mutation- nickase mutant

    Promoter

    CAG

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDESTmycIGF2BP3

    Plasmid

    #19879

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV SOCS-3

    Plasmid

    #11486

    Depositor

    Ronald Kahn

    Article

    Ueki et al Mol Cell Biol. 2004 Jun . 24(12):5434-46

    Insert

    SOCS-3 (Socs3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLIK-Venus

    Plasmid

    #25734

    Purpose

    Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Venus fluorescent protein coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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