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Addgene

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We narrowed to 9,769 results for: 158

Showing: 4411 - 4440 of 9769 results

  2. pEJS-HZ79 All-in-one AAV-U1a-NmeABE8e-2xBPSV40-U6-Rosa26_V2

    Plasmid
    #199261
    Purpose
    Optimized single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one U6 driven sgRNA targeting mouse Rosa26 gene. This optimized construct showed improved in vivo editing efficiency.
    Depositor
    Erik Sontheimer
    Article
    Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.
    Insert
    NmeABE8e
    Use
    AAV and CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Promoter
    U1a promoter
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    199261-plasmid-map-sequence-id-389819

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  6. pcDNA5/FRT/TO-GAlphaQ-RLuc8

    Plasmid
    #140982
    Purpose
    Encodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaQ-RLuc8 (GNAQ Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140982-plasmid-map-sequence-id-300744

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  12. pGHUC w-ERBIN (2-hybrid prey plasmid)

    Plasmid
    #128163
    Purpose
    UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).
    Depositor
    Marcus Noyes
    Article
    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928
    Insert
    ERBIN PDZ domain (ERBIN Synthetic)
    Use
    Synthetic Biology
    Tags
    FLAG and Omega subunit of RNA polymerase
    Expression
    Bacterial
    Mutation
    Promoter
    UV5
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    128163-plasmid-map-sequence-id-267568

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  14. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

    Plasmid
    #100597
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)
    Depositor
    Akitsu Hotta
    Article
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100597-plasmid-map-sequence-id-207349

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  15. Target-AID (pRZ762)

    Plasmid
    #131300
    Purpose
    CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP.
    Depositor
    Keith Joung
    Article
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Insert
    NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-pmCDA1(R187W with reference to NCBI ABO15149.1)-NLS-UGI-P2A-EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Oct. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    131300-plasmid-map-sequence-id-254651

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  16. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

    Plasmid
    #100596
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)
    Depositor
    Akitsu Hotta
    Article
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100596-plasmid-map-sequence-id-207339

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  17. TFORF2090

    Plasmid
    #141916
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FANCD2 (FANCD2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141916-plasmid-map-sequence-id-454923

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  18. pcDNA5/FRT/TO-GAlphaGustducin-RLuc8

    Plasmid
    #140979
    Purpose
    Encodes a G alpha subunit (GNAT3) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaGustducin-RLuc8 (GNAT3 Human)
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140979-plasmid-map-sequence-id-300662

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  22. pTU1-A-pdh_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107576
    Purpose
    B. megaterium DSM319 pdh promoter, GFP+ with malachite green mRNA aptamer in Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    GFP+
    Use
    Synthetic Biology
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    pdh promoter Bacillus megaterium DSM319
    Available since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    107576-plasmid-map-sequence-id-214314

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  27. TFORF0780

    Plasmid
    #141697
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GTF2H1 (GTF2H1 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141697-plasmid-map-sequence-id-404492

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Showing: 4411 - 4440 of 9769 results