We narrowed to 9,393 results for: Coli

1. pCDF_HA_CASP_sigma

   Plasmid

   #197614

   Purpose

   For expression of HA-tagged CASP sigma in E. coli

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2022 Nov 25;378(6622):874-881.

   Insert

   HA-tagged CASP sigma
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJK225

   Plasmid

   #71124

   Purpose

   Expression of Acetobacter aceti 1023 citrate synthase (AaCSH6) in E. coli

   Depositor

   T. J. Kappock

   Article

   Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

   Insert

   citrate synthase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRSETA_crE6G2

   Plasmid

   #172929

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain

   Depositor

   Arnold Boersma

   Article

   Liu et al Biophys J. 2017 May 9;112(9):1929-1939.

   Insert

   CrowdingSensorE6G2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Val317Gly, Met316Gly

   Promoter

   T7

   Available since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PibpA GFP pZa

   Plasmid

   #170076

   Purpose

   GFP expression under the control of E. coli ibpA promoter

   Depositor

   Urartu Seker

   Article

   Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

   Insert

   Green fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PibpA

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PfxsA GFP pZa

   Plasmid

   #170075

   Purpose

   GFP expression under the control of E. coli fxsA promoter

   Depositor

   Urartu Seker

   Article

   Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

   Insert

   Green fluorescent protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PfxsA

   Available since

   Sept. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMD19T-slr0230-PL22-sgRNANT1-KmR

   Plasmid

   #73224

   Purpose

   Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli

   Depositor

   Paul Hudson

   Article

   Yao et al ACS Synth Biol. 2015 Dec 28.

   Insert

   PL22-sgRNA NT1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PL22

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTH1-SaSrtA-Q

   Plasmid

   #64980

   Purpose

   Expresses the E108Q mutant of Staphylococcus aureus sortase A in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

   Insert

   sortase A
   

   Use

   Tags

   His6 tag, Mxe GyrA intein, and chitin-binding pro…

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-59, E108Q

   Promoter

   Available since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMZ2007

   Plasmid

   #204399

   Purpose

   A 3OC6-HSL sensor for B. subtilis on a shuttle vector for B. subtilis and E. coli.

   Depositor

   Lauren Andrews

   Article

   Zeng et al ACS Synth Biol. 2023 Dec 11. doi: 10.102

   Inserts

   quorum sensing regulator LuxR
   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   PftsH and Synthetic promoter PLux0002

   Available since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSEVA221TrxA-AID-T7RNAP

   Plasmid

   #167976

   Purpose

   Expresses fusion protein TrxA-AID-T7RNAP in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

   Insert

   TrxA-AID-T7RNAP
   

   Use

   Tags

   T7RNAP and TrxA

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmMaple2-CAM

    Plasmid

    #101149

    Purpose

    Fluorescent protein for superresolution imaging

    Depositor

    Xiaowei Zhuang

    Article

    Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

    Insert

    E. coli codon optimized mMaple2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CHIKV-nsP3-c001

    Plasmid

    #173086

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    N/A
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    A1334-T1493, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Plas-gRNA-LEU2

    Plasmid

    #157656

    Purpose

    GFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequence

    Depositor

    Frank Albert

    Article

    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

    Insert

    GFP-gRNA
    

    Use

    Insert storage (replicative in e. coli)

    Tags

    GFP-gRNA

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p70rg

    Plasmid

    #17827

    Depositor

    Jay Keasling

    Article

    Pfleger et al Nat Biotechnol. 2006 Aug . 24(8):1027-32

    Insert

    E. coli optimized red flourescent protein (RFPec), GFPuv
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pSecEffector

    Plasmid

    #172891

    Purpose

    Contains TEVp display cassette, atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901

    Depositor

    Urartu Seker

    Article

    Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

    Inserts

    Bxb1
    TP901
    TEV Display cassette
    ProD promoter between anti aligned Bxb1 sites
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ProD, nTETO, and pBAD /AraC

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-nGFPMNase

    Plasmid

    #187826

    Purpose

    express nGFPMNase protein in E coli

    Depositor

    Yikang Rong

    Article

    Stefanis et al Brain Res Mol Brain Res. 1998 Jan;53(1-2

    Insert

    nGFPMNASE
    

    Use

    Tags

    his tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promotor

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pIA1405

    Plasmid

    #172404

    Purpose

    Expression of SARS-COV-2 Nsp12 D218A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    D218A

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pIA1420

    Plasmid

    #172406

    Purpose

    Expression of SARS-COV-2 Nsp12 R116A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    R116A

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pIA1421

    Plasmid

    #172407

    Purpose

    Expression of SARS-COV-2 Nsp12 K50A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pICH75111

    Plasmid

    #50327

    Depositor

    Nicola Patron, Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, beta-glucuronidase (E. coli) with two introns,
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBXPC3H-Syb37

    Plasmid

    #123627

    Purpose

    Expresses Syb37 in e. coli cells, with Arabinose induction and Ampicillin selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    Syb37
    

    Use

    Tags

    3C-His and PelB

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEXP5-NT/pLux LasI

    Plasmid

    #193628

    Purpose

    Inducible expression of LasI by the transcription factor complex LuxR-3OC6 homoserine lactone (HSL). LasI is codon optimized for E. coli.

    Depositor

    Dora Tang

    Article

    Gonzales et al ChemSystemsChem 2023

    Insert

    LasI
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Lux promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTYF-1xSYN-tTA

    Plasmid

    #19980

    Depositor

    Sergey Kasparov

    Article

    Liu et al J Gene Med. 2008 May . 10(5):583-92.

    Insert

    SYN-tTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pIA1402

    Plasmid

    #172403

    Purpose

    Expression of SARS-COV-2 Nsp12 Y129A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Y129A

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJT103

    Plasmid

    #25087

    Depositor

    Christopher Voigt

    Article

    Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

    Insert

    PompC-lacZ
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pETDuet-PcPT-ximD-ximE

    Plasmid

    #172654

    Purpose

    Production of decursinol in E. coli

    Depositor

    Minjuan Xu

    Article

    Bu et al ACS Synth Biol. 2020 Sep 18;9(9):2282-22

    Insert

    PcPT, XimD and XimE
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFLIP38

    Plasmid

    #65616

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-edCit-attR1 and attR2-edCer-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBAD-pnGFP-Ultra

    Plasmid

    #157923

    Purpose

    Peroxynitrite sensor pnGFP-Ultra in E. coli (use with pEvol-BoF)

    Depositor

    Huiwang Ai

    Article

    Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-

    Insert

    pnGFP-Ultra, a high performance peroxynitrite sensor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pICSL80016

    Plasmid

    #50332

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS no stop codon, beta-glucuronidase (E. coli) with two introns
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFLIP43

    Plasmid

    #65618

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-edAFPt9-attR1 and attR2-edCer-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pQE80_T5_P3-ehaA_LacI

    Plasmid

    #186315

    Purpose

    Plasmid for inducible E coli expression of the P3 helixCAM.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Insert

    P3-ehaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pQE80_T5_AP4-ehaA_LacI

    Plasmid

    #186316

    Purpose

    Plasmid for inducible E coli expression of the AP4 helixCAM.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Insert

    AP4-ehaA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pIA1408

    Plasmid

    #172405

    Purpose

    Expression of SARS-COV-2 Nsp12 D760A in E. coli

    Depositor

    Irina Artsimovitch

    Article

    Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

    Insert

    SARS-COV-2 Nsp12 (ORF1ab Synthetic)
    

    Use

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    D760A

    Promoter

    T7

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Plas-CRISPR_reporter

    Plasmid

    #157658

    Purpose

    CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene

    Depositor

    Frank Albert

    Article

    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

    Insert

    dCAS9-VP64, mCherry, KanMX
    

    Use

    Insert storage (replicative in e. coli)

    Tags

    Expression

    Mutation

    N28D in mCherry- please see depositor comments be…

    Promoter

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pReg-ROTU4

    Plasmid

    #190669

    Purpose

    Expresses an evolved rotundine biosensor in E. coli

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    ROTU4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGfOMT1-GEN4

    Plasmid

    #190612

    Purpose

    Expresses an evolved methyltransferase for tetrahydropapaverine biosynthesis in E. coli

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    GfOMT-GEN4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pReg-GLAU4

    Plasmid

    #190667

    Purpose

    Expresses an evolved glaucine biosensor in E. coli

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    GLAU4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pReg-PAP4

    Plasmid

    #190668

    Purpose

    Expresses an evolved papaverine biosensor in E. coli

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    PAP4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pReg-NOS4

    Plasmid

    #190670

    Purpose

    Expresses an evolved noscapine biosensor in E. coli

    Depositor

    Andrew Ellington

    Article

    d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

    Insert

    NOS4
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pVY-3 sfGFP-tag3

    Plasmid

    #194618

    Purpose

    fluorescent proteins with cationic peptide tags to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

    Insert

    sfGFP-K(GGSKKRKKR)3
    

    Use

    Tags

    KGGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFLIP37

    Plasmid

    #65505

    Purpose

    FRET biosensor Destination vector for E. coli expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-Cit-attR1 and attR2-Cer-cMyc

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge