We narrowed to 18,361 results for: met

1. pcDNA-PhoCl-mCherry-myc

   Plasmid

   #87692

   Purpose

   Express PhoCl-mCherry with C-term myc tag in mammalian cells

   Depositor

   Robert Campbell

   Article

   Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   PhoCl-mCherry-myc
   

   Use

   Tags

   myc tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPD005 pcDNA Golden Gate

   Plasmid

   #138749

   Purpose

   Vector used to express most COMET components, modified from pcDNA3.1/HygroR(+) for BsaI/BpiI Golden Gate compatibility and to lack HygroR.

   Depositor

   Joshua Leonard

   Article

   Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-SMVP-Cas9N-U6-gRNA P2

   Plasmid

   #80944

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA P2 for Pd-l1 binding

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. mMaroon-RTK-mMaroon

   Plasmid

   #84359

   Purpose

   FRET acceptor for RhoA donor, flanked with the far-red FP mMaroon

   Depositor

   Ryohei Yasuda

   Article

   Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mMaroon-Rtk--mMaroon
   

   Use

   Tags

   mMaroon flanking Rtk domain on N and C terminus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDH_SBP-mCherry-Ecadherin

   Plasmid

   #65291

   Purpose

   Ecadherin reporter only (no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Ecadherin
   

   Use

   Lentiviral

   Tags

   IL-2 Signal Sequence and Streptavidin Binding Pro…

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCytERM_mScarlet-H_N1

   Plasmid

   #85067

   Purpose

   In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   CytERM
   

   Use

   Tags

   mScarlet-H

   Expression

   Mammalian

   Mutation

   aa1-29

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV2-hNCOA6:V5

   Plasmid

   #63585

   Purpose

   A subunit of the Trithorax-related (Trr) methyltransferase complex.

   Depositor

   Kenneth Irvine

   Article

   Oh et al Cell Rep. 2014 Jul 24;8(2):449-59. doi:

   Insert

   NCOA6 (NCOA6 Human)
   

   Use

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

8. CL7-LbCas12aD156R

   Plasmid

   #164659

   Purpose

   Bacterial expression of mutated LbCas12aD156R using CL7/Im7

   Depositor

   Piyush Jain

   Article

   Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

   Insert

   LbCas12a-D156R
   

   Use

   Tags

   Thioredoxin, CL7, SUMO

   Expression

   Bacterial

   Mutation

   changed Aspartic Acid 156 to Arginine

   Promoter

   T7

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTriEx-mCherry-LOVSD

   Plasmid

   #81033

   Purpose

   Mammalian expression of the super-dark state of LOV2

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   LOVSD
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   C450A, L514K, G528A, L531E, N538E

   Promoter

   Available Since

   Aug. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV TBG FFLuc

    Plasmid

    #35658

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TBG

    Available Since

    July 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRNA-U6-let7 sponge

    Plasmid

    #35664

    Depositor

    Phillip Zamore

    Article

    Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

    Insert

    U6 let-7sponge
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    June 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCVL SFFV HA.NLS.I-AniIY2(reo).T2A.TagBFP

    Plasmid

    #31477

    Depositor

    Andrew Scharenberg

    Article

    Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

    Insert

    SFFV HA NLS Y2 T2A mTagBFP
    

    Use

    Lentiviral

    Tags

    HA and NLS

    Expression

    Mutation

    F13Y + S111Y on I-AniI = Y2 "reo" (res…

    Promoter

    Available Since

    Aug. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMK401 (Hygro-P2A-mAID-Nluc)

    Plasmid

    #121196

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID-Nluc
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLI-N-GFP-2xFKBP-loxP(K13)

    Plasmid

    #85792

    Purpose

    P. falciparum integration plasmid for N-terminal gene fusions and placing loxP sites using SLI

    Depositor

    Tobias Spielmann

    Article

    Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    Kelch13
    

    Use

    Cre/Lox; P. falciparum genomic integration

    Tags

    2xFKBP, GFP

    Expression

    Mutation

    Promoter

    none

    Available Since

    March 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1

    Plasmid

    #81133

    Purpose

    mammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domain

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Inserts

    Zdk1
    VAV2 DH/PH/C1 (VAV2 Human)
    

    Use

    Tags

    mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. SBP-EGFP-Ecadherin_puromycin

    Plasmid

    #65292

    Purpose

    Ecadherin reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Use

    Tags

    EGFP and IL-2 Signal Sequence and Streptavidin Bi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDS277

    Plasmid

    #34973

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(V529N mutation).

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    V529N

    Promoter

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTE103

    Plasmid

    #59398

    Purpose

    MethyloBrick expression vector, fumC promoter; TcR

    Depositor

    Tobias Erb

    Article

    Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PfumC

    Available Since

    Dec. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCFJ1201

    Plasmid

    #44483

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, unc-119, peel-1, MCS
    

    Use

    Multiple cloning site vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. UMG-SP-1_pET29b

    Plasmid

    #215433

    Purpose

    Expression construct for UMG-SP-1 gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    UMG-SP-1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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