We narrowed to 26,178 results for: STI

1. pAAV-CaMKII-Chronos-GFP

   Plasmid

   #58805

   Purpose

   AAV expression of Chronos-GFP under the CaMKII promoter

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBRY-nuclear mCherry-IRES-PURO

   Plasmid

   #52409

   Purpose

   Constitutively expressed mammalian expression vector encoding nuclear mCherry fluorescent reporter. Puromycin resistance.

   Depositor

   Jacob Hanna

   Article

   Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

   Insert

   nuclear mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-FLEX-rc[Chronos-GFP]

   Plasmid

   #59056

   Purpose

   Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. Gene Trap - 24XPP7 Lentiviral Vector

   Plasmid

   #174199

   Purpose

   Lentiviral expression vector coding for Blasticidin, iRFP and 24X PP7 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.

   Depositor

   Daniel Larson

   Article

   Wan et al Cell. 2021 May 27;184(11):2878-2895.e20.

   Insert

   iRFP
   

   Use

   Lentiviral; Vector

   Tags

   24X PP7 hairpins, Blasticidin, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. FCK-Chronos-GFP

   Plasmid

   #80902

   Purpose

   Lenti-mediated expression of Chronos-GFP under the CaMKII promoter.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available since

   March 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUC19-U6-BsmBI_cassette-CjeCas9-sgRNA (KAC482)

   Plasmid

   #133793

   Purpose

   U6 promoter sgRNA entry vector used for all CjeCas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V2-TGAA linker sgRNA architecture from Kim et al. Nature Communications 2017

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   CjeCas9 sgRNA entry vector
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   V2-TGAA linker sgRNA architecture from Kim et al.…

   Promoter

   U6

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. BtARR1 Cys-less

   Plasmid

   #236274

   Purpose

   Bacterial expression of bovine arrestin-1 C63A, C128S, C143A, inserted GGT codon after starting ATG

   Depositor

   Vsevolod Gurevich

   Article

   Hanson et al Proc Natl Acad Sci U S A. 2006 Mar 28;10

   Insert

   arrestin-1 (SAG Bovine)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   C63A, C128S, C143A, inserted GGT codon after star…

   Promoter

   lac

   Available since

   May 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hKCNB1 L211P

   Plasmid

   #124487

   Purpose

   missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

   Depositor

   Federico Sesti

   Article

   Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

   Insert

   human KCNB1 (KCNB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed leucine 211 to proline

   Promoter

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hKCNB1 G381R

   Plasmid

   #124490

   Purpose

   missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

   Depositor

   Federico Sesti

   Article

   Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

   Insert

   human KCNB1 (KCNB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed glycine at 381 to arginine

   Promoter

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hKCNB1 F416L

    Plasmid

    #124491

    Purpose

    missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

    Depositor

    Federico Sesti

    Article

    Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

    Insert

    human KCNB1 (KCNB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed phenyalanine 416 to leucine

    Promoter

    Available since

    Sept. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. hKCNB1 G379R

    Plasmid

    #124489

    Purpose

    missense mutation in children with EOEE. HA tagged in C-terminus, pCIneo vector

    Depositor

    Federico Sesti

    Article

    Yu et al FASEB J. 2019 Dec;33(12):14680-14689. do

    Insert

    human KCNB1 (KCNB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed glycine at 379 to arginine

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. csd6_13-330_H160A/pET28b(+) N-tag

    Plasmid

    #71359

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:H160A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Histidine 160 to Alanine)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

13. csd6_13-330_H155A/pET28b(+) N-tag

    Plasmid

    #71357

    Purpose

    csd6, construct:13-330, pET28b(+) N-tag, mutation:H155A

    Depositor

    SeWon Suh

    Article

    Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

    Insert

    HP0518 (HP0518 changed Histidine 155 to Alanine)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pET21b(+)-Is-PETase

    Plasmid

    #112202

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12

    Depositor

    Christopher Johnson, Gregg Beckham

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
    

    Use

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    Promoter

    N/A

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCMV-T7-ABEmax(7.10)-SpCas9-NG-P2A-EGFP (RTW4564)

    Plasmid

    #140005

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9-NG with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-Syn-Chronos-GFP

    Plasmid

    #59170

    Purpose

    AAV-mediated expression of Chronos-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CAG-Chronos-GFP

    Plasmid

    #99232

    Purpose

    AAV-mediated expression of Chronos-GFP under the CAG promoter. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-CBE4max-xCas9(3.7)-P2A-EGFP (RTW4637)

    Plasmid

    #140000

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max xCas9(3.7) with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

    Promoter

    CAG

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCV-TASL-PLPLR

    Plasmid

    #221266

    Purpose

    Expression of TASL-STING PLPLR in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    fusion of TASL with STING PLPLR (Sting1, Tasl Mouse)
    

    Use

    Retroviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AF20-double-tag

    Plasmid

    #108389

    Purpose

    Express human PC1

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Use

    Tags

    eGFP-TEV cleavage-S15 and mcherry-TEV cleavage-V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA5-eGFP-PC1-3HA

    Plasmid

    #108406

    Purpose

    Can be used to generate stable Flp-In cells with tetracycline-inducible human PC1 expression.

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Use

    Tags

    3HA and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-mPC1_4077-eGFP-3HA

    Plasmid

    #108390

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    Pkd1 (Pkd1 Mouse)
    

    Use

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3-mPC1_4119-3HA

    Plasmid

    #108395

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    Pkd1 (Pkd1 Mouse)
    

    Use

    Tags

    3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CBSH3- 3m shRNA3

    Plasmid

    #160213

    Purpose

    Knock Down MYDSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA3 targeting collybistin MYD-CBSH3- isoforms (Plasmid #160212)

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    TGTATGACCTCaGGcTGtACCA (mutations shown in lower …

    Promoter

    mouse U6 promoter

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CBSH3- 3m shRNA2

    Plasmid

    #160211

    Purpose

    Knock Down MTLSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Plasmid #160210)

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    TGACGTTGCTCaGGcTGtACCA (mutations shown in lower …

    Promoter

    mouse U6 promoter

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CBSH3- 3m shRNA1

    Plasmid

    #160209

    Purpose

    Knock Down MQWSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Plasmid # 160208).

    Depositor

    Angel de Blas

    Article

    George et al J Neurochem. 2021 May;157(4):1032-1051.

    Insert

    ARHGEF9 (Arhgef9 Rat)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    GATCGGGAATGCTCaGGcTGtACC (mutations shown in lowe…

    Promoter

    mouse U6 promoter

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-mPC1_4119-eGFP-3HA

    Plasmid

    #108393

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    Pkd1 (Pkd1 Mouse)
    

    Use

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3-mPC1_4077-3HA

    Plasmid

    #108392

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    Pkd1 (Pkd1 Mouse)
    

    Use

    Tags

    3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3-mPC1_4077-eGFP

    Plasmid

    #108391

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4077 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid

    #139999

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CAG

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

    Plasmid

    #185910

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpCas9-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A

    Promoter

    CMV and T7

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLX_TRC311/NLS-Cas13d-P2A-Blast

    Plasmid

    #212196

    Purpose

    CasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40

    Depositor

    Stacey Edwards

    Article

    Wang et al Mol Cancer. 2024 May 15;23(1):101. doi:

    Insert

    CasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
    

    Use

    CRISPR and Lentiviral

    Tags

    HA, NLS, and blasticidin S deaminase

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

    Plasmid

    #223137

    Purpose

    pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-U6-SpCas9_gRNA(RHO-P23H)-CMV-mTagBFP2_(RAS3613)

    Plasmid

    #228252

    Purpose

    pU6 (human U6) expression of SpCas9 sgRNA targeting RHO P23H mutation and pCMV expression of mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    SpCas9 P23H sgRNA, mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CMV and U6

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

    Plasmid

    #228253

    Purpose

    pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    U6

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid

    #140002

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET-21b(+)-Is-PETase-W185A

    Plasmid

    #112204

    Purpose

    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W185A mutations, codon optimized for expression in E. coli K12

    Depositor

    Christopher Johnson, Gregg Beckham

    Article

    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

    Insert

    PETase gene from Ideonella sakaiensis 201-F6 with W185A mutation, codon optimized for expression in E. coli K12
    

    Use

    Tags

    6XHIS

    Expression

    Bacterial

    Mutation

    W185A

    Promoter

    N/A

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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38. pCMV-T7-ABE8.20m-nSpCas9-P2A-EGFP (KAC1158)

    Plasmid

    #185915

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8.20m-nSpCas9-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. FUW-Flag-Mbd3

    Plasmid

    #52371

    Purpose

    Lentiviral expression vector for constitutive expression of double Flag tagged Mbd3

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Ub

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAAV-EF1α1.1-FLPX-rc [Chronos-GFP]

    Plasmid

    #122102

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a（1.1kb short version)

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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41. pCMV-T7-ABEmax(7.10)-xCas9(3.7)-P2A-EGFP (RTW4644)

    Plasmid

    #140004

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with xCas9(3.7)(D10A/A262T/R324L/S409I/E480K/E543D/M694I/E1219V) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) xCas9(3.7) with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; xCas9(3.7)=A262T/R324L/S409I/E480K/…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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42. FUW-Mbd3

    Plasmid

    #52356

    Purpose

    Lentiviral expression vector for constitutive expression of Mbd3

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    Mbd3 (Mbd3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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43. pAAV-EF1α-FLEX-rc[Chronos-GFP]

    Plasmid

    #62725

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    April 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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44. pcDNA3-hPC1_4106-eGFP-3HA

    Plasmid

    #108399

    Purpose

    Express cytoplasmic tail human PC1 from amino acid 4106 to 4302

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Use

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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45. pcDNA3-CD16.7-hPC1-eGFP-3HA

    Plasmid

    #108401

    Purpose

    Express cytoplasmic tail human PC1 from amino acid 4077 to 4302 with a membrane anchor

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Use

    Tags

    eGFP-3HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. FUW-CAGGS-ERAS

    Plasmid

    #52416

    Purpose

    Lentiviral vector encoding constitutive expression of human ERAS cDNA

    Depositor

    Jacob Hanna

    Article

    Rais et al Nature. 2013 Oct 3;502(7469):65-70. doi:

    Insert

    ERAS (ERAS Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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47. pAAV-EF1α1.1-Chronos-GFP

    Plasmid

    #122098

    Purpose

    AAV-mediated expression of Chronos-GFP under the EF1α promoter (1.1kb short version). Using SV40 pA signal.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α (1.1 kb short version)

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pcDNA3-hPC1_MTS_L4139P-eGFP-3HA

    Plasmid

    #108404

    Purpose

    Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157 with a likely pathogenic point mutation.

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Human)
    

    Use

    Tags

    3xHA and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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49. pcDNA3-mPC1_4119-eGFP

    Plasmid

    #108394

    Purpose

    Genomic region, including intron 45, to express cytoplasmic tail mouse PC1 from amino acid 4119 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    PKD1 (PKD1 Mouse)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    variant ID rs13468401 missense variant R4237Q

    Promoter

    CMV

    Available since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pcDNA3-mPC1_4148-3HA

    Plasmid

    #108398

    Purpose

    Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

    Depositor

    Gregory Germino

    Article

    Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

    Insert

    Pkd1 (Pkd1 Mouse)
    

    Use

    Tags

    3HA

    Expression

    Mammalian

    Mutation

    variant ID rs13468401 missense variant R4237Q

    Promoter

    CMV

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge