We narrowed to 10,302 results for: 158

1. pYPQ146-ZmUbi-tRNA

   Plasmid

   #158404

   Purpose

   Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ136-tRNA2.0; assembly of 6 gRNAs

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Maize ubiquitin 1

   Available since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX-4T2-Rheb

   Plasmid

   #15889

   Depositor

   John Blenis

   Article

   Tee et al Curr Biol. 2003 Aug 5. 13(15):1259-68.

   Insert

   Rheb (RHEB Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 26, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pLKO_HA.NWS.VSVg_NGFR

   Plasmid

   #158243

   Purpose

   Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

   Depositor

   Brian Brown

   Article

   Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

   Insert

   Pro-Code Tagged human dNGFR (NGFR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   HA.NWS.VSVg

   Expression

   Mutation

   Truncation of the signaling domain

   Promoter

   EF1a

   Available since

   Sept. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYPQ-dpcoCas9-Act3.0

   Plasmid

   #158408

   Purpose

   CRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.

   Depositor

   Yiping Qi

   Article

   Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

   Insert

   dpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CMV-CitroffRH

   Plasmid

   #158546

   Purpose

   Mammalian expression of nonbinding variant of inverse-response citrate sensor

   Depositor

   Robert Campbell

   Article

   Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

   Insert

   CitroffRH
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   Oct. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLEX307-TMPRSS11D-blast

   Plasmid

   #158476

   Purpose

   Constitutive lentiviral expression of human TMPRSS11D.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   TMPRSS11D (TMPRSS11D Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLKO-Cre sgAmotl2 v3

   Plasmid

   #158602

   Purpose

   lenti-viral construct with Cas9, Cre recombinbase and U6 driven sgRNA against mouse Amotl2

   Depositor

   Daniel Schramek

   Article

   Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

   Insert

   Amotl2 sgRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 30, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pDONR221-APN

   Plasmid

   #158440

   Purpose

   Gateway Cloning compatible entry vector for the human APN gene.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   APN (ANPEP Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-mas-KB-1753-Nluc

   Plasmid

   #158603

   Purpose

   Plasmid expressing KB-1753-Nluc in mammalian cells

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pDONR221-ACE2

    Plasmid

    #158438

    Purpose

    Gateway Cloning compatible entry vector for the human ACE2 gene.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLIX403-ccdB-EYFP

    Plasmid

    #158564

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pyc111f

    Plasmid

    #158780

    Purpose

    ribosome-tagged, Cre-dependent GCaMP for soma localization

    Depositor

    Jennifer Garrison, Zachary Knight

    Article

    Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

    Insert

    GCaMP6f-RPL10a
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYC1446

    Plasmid

    #158720

    Purpose

    Integrating plasmid expressing Sth1 Cas9 and the cognate sgRNA

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    Sth1 sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO_HA.FLAG.VSVg_NGFR

    Plasmid

    #158245

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    HA.FLAG.VSVg

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-Syn-SomaGCaMP6f1

    Plasmid

    #158755

    Purpose

    Cell body-targeted GCaMP6f under synapsin promoter: GCaMP6f followed by a linker, the tail region of AnkyrinG, and an ER export motif (GCaMP6f-27-AnkTail-motif-ER2). Not as bright as SomaGCaMP6f2.

    Depositor

    Edward Boyden

    Article

    Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

    Insert

    SomaGCaMP6f1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    synapsin promoter

    Available since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p663-UBC-miniTurbo-V5-CDK2_IDG-K

    Plasmid

    #158193

    Purpose

    Gateway destination clone of CDK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-CDK2 (CDK2 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLIX403-EYFP-ccdB-Blast

    Plasmid

    #158551

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MAC-BSG

    Plasmid

    #158383

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    BSG (BSG Human)
    

    Use

    Tags

    MAC-tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pLKO_NWS.FLAG.VSVg_NGFR

    Plasmid

    #158233

    Purpose

    Protein Barcode (Pro-Code) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables phenotypic CRISPR screens at a single cell resolution (using cytometry).

    Depositor

    Brian Brown

    Article

    Wroblewska et al Cell. 2018 Nov 1;175(4):1141-1155.e16. d

    Insert

    Pro-Code Tagged human dNGFR (NGFR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    NWS.FLAG.VSVg

    Expression

    Mutation

    Truncation of the signaling domain

    Promoter

    EF1a

    Available since

    Sept. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 6x-His_A1aY1_GFP

    Plasmid

    #158753

    Purpose

    Bacterial expression of 6xHis-A1aY1 fused with carboxy terminus GFP for recombinant purification

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

    Insert

    6xHistidine_A1aY1_GFP
    

    Use

    Tags

    6x-Histidine tag, GFP, T7 leader sequence, and Th…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge