We narrowed to 2,455 results for: GEM

1. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

   Plasmid

   #176932

   Purpose

   Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hGEM(1-110)_mNG_hSLBP(18-108)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK1

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEM-sgMUC1_dual-MTS-3x-Set I

   Plasmid

   #162763

   Purpose

   Separately expressing 3 unique sgMUC1_dual-MTS (SetI)

   Depositor

   Antony K Chen

   Article

   Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

   Insert

   sgMUC1_dual-MTS_1; sgMUC1_dual-MTS_2; sgMUC1_dual-MTS_3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCashGem-Xyl2-URA3-LINEAR

   Plasmid

   #174840

   Purpose

   pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   Codon optimized Cas9:hGem
   

   Use

   CRISPR and Synthetic Biology

   Tags

   hGeminin tag

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   ENO1p

   Available since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEM-3XP3.RFP-tan

   Plasmid

   #83474

   Purpose

   RFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.

   Depositor

   Patricia Wittkopp

   Article

   Lamb et al Fly (Austin). 2016 Aug 5:1-12.

   Insert

   3XP3.RFP-tan
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   3XP3 eye-expression Promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEMHE-mClover3-MAP4-MTBD

   Plasmid

   #171498

   Purpose

   T7 promotor drives in vitro transcription of mClover3-tagged mouse MAP4-Microtubule Binding Domain mRNA

   Depositor

   Melina Schuh

   Article

   So et al Science. 2019 Jun 28;364(6447). pii: 364

   Insert

   MAP4-MTBD (Map4 Mouse)
   

   Use

   Pgehme

   Tags

   mClover3

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1+ GEMIN6 myc HIS

   Plasmid

   #71659

   Purpose

   Mammalian expression of GEMIN6

   Depositor

   Gideon Dreyfuss

   Insert

   GEMIN6 (GEMIN6 Human)
   

   Use

   Tags

   HIS and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

7. Rab27a cds in pGem-T Easy

   Plasmid

   #80546

   Purpose

   Can be used for in situ hybridisation or other purposes

   Depositor

   Rob Parton

   Article

   Hall et al Cell Logist. 2017 Mar 1;7(1):e1301151. d

   Insert

   rab27a (rab27a Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEMHE-mCherry-mTrim21ΔRING-Box

   Plasmid

   #105523

   Purpose

   T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mCherry

   Expression

   Mutation

   deletion of RING domain (aa124-462)

   Promoter

   T7

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGem-PgpdA-Cre-TtrpC

   Plasmid

   #168784

   Purpose

   Helper vector for the transient expression of Cre recombinase in filamentous fungi. Not replicative in fungi.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

   Insert

   Cre
   

   Use

   Cre/Lox and Synthetic Biology; Expression in fila…

   Tags

   Expression

   Mutation

   Promoter

   PgpdA

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9_hGEM(1-110)_hSLBP(56-108)

    Plasmid

    #191388

    Purpose

    SpCas9 fused with geminin and SLBP degrons to improve HDR ratio

    Depositor

    Yannick Doyon

    Insert

    hGEM(1-110)_hSLBP(56_108)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEMHE-mEGFP-mTrim21ΔRING-Box

    Plasmid

    #105520

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted RING-box mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Expression

    Mutation

    deletion of RING domain (aa124-462)

    Promoter

    T7

    Available since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEMT-Map7-1333-end

    Plasmid

    #46070

    Depositor

    Edgar Gomes

    Article

    Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

    Insert

    Map7-1333-end (Map7 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLeo-A-a2-GEMS(JAK-STAT)

    Plasmid

    #209110

    Purpose

    Transient mammalian expression of the CC functionalized GEMS receptor A-a2-GEMS (JAK-STAT)

    Depositor

    Tom de Greef

    Article

    Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

    Insert

    A-a2-GEMS(JAK-STAT)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEM mouse PEA3 [TJ#200]

    Plasmid

    #16346

    Depositor

    Thomas Jessell

    Insert

    PEA3 (Etv4 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

15. KM-CashGem-Xyl2-URA3-LINEAR

    Plasmid

    #174838

    Purpose

    pRS414 backbone containing LINEAR fragment targeting XYL2 in K. marxianus

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TEF1p

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEM-and1 in situ probe

    Plasmid

    #52102

    Purpose

    actinodin 1 and1 antisense in situ probe linearize w/ ApaI SP6 pol

    Depositor

    Didier Stainier

    Article

    Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

    Insert

    actinodin 1 in situ probe (and1 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. YL-CashGem-BAT1-URA3-LINEAR

    Plasmid

    #174837

    Purpose

    pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    Codon optimized Cas9:hGem
    

    Use

    CRISPR and Synthetic Biology

    Tags

    hGeminin tag

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GPDp

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEM-sgMUC4_dual-MTS-3x-Set I

    Plasmid

    #162762

    Purpose

    Separately expressing 3 unique sgMUC4_dual-MTS (SetI)

    Depositor

    Antony K Chen

    Article

    Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

    Insert

    sgMUC4_dual-MTS_1; sgMUC4_dual-MTS_2; sgMUC4_dual-MTS_3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. HP-Cas9hGem-Ade2-LEU2-LINEAR

    Plasmid

    #174839

    Purpose

    pRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorpha

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TDH3p

    Available since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEM-T Easy-Gbait-hs-QF2

    Plasmid

    #122563

    Purpose

    Integration of QF2 sequence using the CRISPR/Cas9 system.

    Depositor

    Marnie Halpern

    Article

    Choi et al Elife. 2021 Dec 8;10:e72345. doi: 10.755

    Insert

    Gbait-hsp70-QF2-SV40pA
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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