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171,784 results

Showing: 4471 - 4500 of 171784 results

  3. pDOC-K-glmS-GFPrev

    Plasmid
    #158060
    Purpose
    Donor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable marker
    Depositor
    Mark Webber
    Article
    Holden et al Microbiology (Reading). 2020 Nov 23. doi
    Insert
    Green Fluorescent Protein optimised for excitation with UV light
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    acpP
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    158060-plasmid-map-sequence-id-309440

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  4. 4QL1

    Plasmid
    #61942
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    WDR5:APC041-G05:C36415 (WDR5 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. pSBbi-GB

    Plasmid
    #60520
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: GFP and blasticidin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Expression
    Mammalian
    Promoter
    EF1a/RPBSA
    Available Since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60520-plasmid-map-sequence-id-97526

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  8. pDRM210 LGP (Luciferase GFP Puro)

    Plasmid
    #174722
    Purpose
    Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene
    Depositor
    Wayne Tilley
    Article
    Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413
    Inserts
    Firefly Luciferase
    eGFP
    pac
    Use
    Lentiviral and Luciferase
    Tags
    E2A linker and F2A linker
    Expression
    Mammalian
    Promoter
    MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…
    Available Since
    Nov. 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    174722-plasmid-map-sequence-id-348963

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  15. ECFP HO

    Plasmid
    #207413
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    ECFP
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207413-plasmid-map-sequence-id-421043

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  16. ECFP CD

    Plasmid
    #207414
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    ECFP
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207414-plasmid-map-sequence-id-421036

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  18. pcDNA GNSTM-3-RVG-10-Lamp2b-HA

    Plasmid
    #71294
    Purpose
    Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.
    Depositor
    Joshua Leonard
    Article
    Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72
    Insert
    Lamp2b (LAMP2 Human)
    Tags
    GNSTM glycosylation motif, HA, Lamp2 signal pepti…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71294-plasmid-map-sequence-id-418480

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  22. Td-CGBE

    Plasmid
    #196599
    Purpose
    Mammalian expression of Td-CGBE
    Depositor
    Dali Li
    Article
    Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103
    Insert
    bpNLS-TadA-8e (N46L)-32aa linker-nSpCas9-bpNLS
    Tags
    6*His
    Expression
    Mammalian
    Mutation
    TadA-8e (N46L); S. pyogenes Cas9 (D10A)
    Available Since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196599-plasmid-map-sequence-id-386792

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  23. IDG_TMEM63B_OE_1

    Plasmid
    #161682
    Purpose
    Overexpresses TMEM63B
    Depositor
    Mike McManus
    Insert
    TMEM63B (TMEM63B Human)
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    161682-plasmid-map-sequence-id-319709

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  27. pET30a-His6-LOXCAT

    Plasmid
    #140084
    Purpose
    Fusion of A. viridans lactate oxidase (LOX) and E. coli catalase (CAT) that converts lactate into pyruvate without producing H2O2.
    Depositor
    Vamsi Mootha
    Article
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Tags
    His6
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140084-plasmid-map-sequence-id-311164

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  30. NG-ABE9

    Plasmid
    #240625
    Purpose
    Adenine base editor with narrowed editing window, recognizing NG PAM
    Depositor
    Tingting Jiang
    Article
    Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:
    Insert
    TadA8e (N108Q/L145T)-linker-nCas9 (D10A, L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T1337R)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    TadA8e (N108Q/L145T), Cas9 (D10A, L1111R, D1135V,…
    Available Since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    240625-plasmid-map-sequence-id-479197

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Showing: 4471 - 4500 of 171784 results