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pX330A-1x2

Plasmid

#58766

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-mIAA7-ALFA-HIS3MX6

Plasmid

#247627

Purpose

Yeast gene tagging with mIAA7

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

mIAA7

Tags

ALFA

Expression

Yeast

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

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SpyCatcher002-oDi

Plasmid

#124661

Purpose

Expresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coil

Depositor

Mark Howarth

Article

Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

Insert

SpyCatcher002-oDi

Tags

C-tag, His6, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab7A

Plasmid

#28047

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2010 Nov 9. 10

Insert

Rab7A (RAB7A Human)

Tags

2xHA and EGFP

Expression

Mammalian

Available Since

May 27, 2011

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-mCherry-EGFP-LC3B

Plasmid

#170446

Purpose

Lentiviral vector expressing tandem mCherry-EGFP-LC3B in mamalian cell. To visualize free autophagosome and autophagosomes that have fused with the lysosome.

Depositor

Sang-Hun Lee

Article

Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

Insert

mCherry-GFP-LC3 (MAP1LC3B Human)

Use

Lentiviral

Promoter

EF1a

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCX459

Plasmid

#229214

Purpose

CRISPR array plasmid for MAP1B (Spacers 1-24)

Depositor

Jennifer Doudna

Article

Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

Insert

CRISPR array plasmid for MAP1B (Spacers 1-24)

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-Tol1.zf1

Plasmid

#61388

Purpose

mRNA synthesis of tol1 transposase codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

tol1.zf1

Use

Zebrafish expression

Promoter

sp6

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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Watermelon backbone

Plasmid

#155258

Purpose

Can be used to generate a Watermelon library

Depositor

Joan Brugge, Aviv Regev

Article

Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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PB-TRE-dCas9-KRAB-MeCP2

Plasmid

#122267

Purpose

SP-dCas9-KRAB-MeCP2 with doxycycline-inducible expression

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

dCas9-KRAB-MeCP2

Tags

dCas9-KRAB-MeCP2

Expression

Mammalian

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-BFP-U6-sgRNA

Plasmid

#196725

Purpose

Sortable sgRNA expression, Chen tracrRNA PMID: 24360272

Depositor

Bernhard Schmierer

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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prhaBAD-anti-rabbit-Nb-Hia5

Plasmid

#239627

Purpose

Rhamnose inducible expression plasmid for anti-rabbit nanobody fusion to Hia5 DNA adenine methyltransferase

Depositor

Aaron Straight

Article

Sidhwani et al bioRxiv [Preprint]. 2025 Jul 4:2025.07.0

Insert

Hia5

Tags

6HIS, MBP, TEV, and anti-Rabbit Nanobody

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Promoter

rhaBAD

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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YE1-BE4max-NG

Plasmid

#138159

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE1)-nSpCas9(NG)-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R126E; within Cas9 D10A + …

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR

Plasmid

#116731

Purpose

Lentiviral expression of EGFR

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Hygro empty (w214-1)

Plasmid

#17484

Purpose

3rd gen lentiviral empty control vector, CMV/TO promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pRU41

Plasmid

#167663

Purpose

ENTRY vector for gRNA1 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-TetO-hNGN2-Puro

Plasmid

#79049

Purpose

Expresses human NEUROGENIN2 (hNGN2) and puromycin resistance gene under control of TetON promoter. This lentiviral vector is used to generate NGN2-iNs (induced neurons) from hiPSCs and hiPSC-NPCs.

Depositor

Kristen Brennand

Article

Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

Insert

hNGN2-P2A-PuroR (NEUROG2 Human)

Use

Lentiviral

Promoter

TetO

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-HaloTag7-LC3

Plasmid

#184899

Purpose

Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Tags

HaloTag7

Expression

Mammalian

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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TRUPATH Triple Gai1

Plasmid

#196048

Purpose

Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

Depositor

Justin English

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Inserts

GAlphai1-RLuc8 (GNAI1 Human)
GGamma9-GFP2
GBeta3 (GNB3 Human)

Use

Luciferase

Tags

GFP2 and GSAG linker

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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p2NIL

Plasmid

#20188

Depositor

Tanya Parish

Article

Parish et al Microbiology (Reading). 2000 Aug;146 ( P

Type

Empty backbone

Use

Suicide vector

Available Since

May 22, 2009

Availability

Academic Institutions and Nonprofits only

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HDAC3 Flag

Plasmid

#13819

Purpose

Mammalian expression of human histone deacetylase 3 with flag tag

Depositor

Eric Verdin

Article

Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

Insert

HDAC3 (HDAC3 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pACBSR-hyg

Plasmid

#87830

Purpose

Expresses lambda-red system with hyg selection marker

Depositor

Pep Charusanti

Article

Huang et al BMC Res Notes. 2014 Jan 8;7:13. doi: 10.

Insert

hyg

Tags

None

Expression

Bacterial

Mutation

None

Promoter

bla

Available Since

Aug. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pRU42

Plasmid

#167664

Purpose

ENTRY vector for gRNA2 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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GFP scFv [N86/38]

Plasmid

#182095

Purpose

Mammalian Expression of GFP scFv. Derived from hybridoma N86/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

recombinant mouse scFv targeting GFP

Tags

HA, His

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-mTurquoise-MLC-IRES-neo

Plasmid

#85145

Purpose

lentiviral expression of myosin light chain (MLC, MYL9), N-terminally tagged with mTurquoise

Depositor

Tobias Meyer

Article

Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

Insert

mTurquoise-MLC (myosin regulatory light chain, N-terminally tagged with mTurquoise (MYL9 Human)

Use

Lentiviral

Tags

mTurquoise

Promoter

EF1a

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pLVET-IRES-GFP

Plasmid

#107139

Purpose

A bicistronic lentiviral mammalian expression vector with a multiple cloning site and GFP

Depositor

Aki Manninen

Article

Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A

Plasmid

#86674

Purpose

Expresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

GFP-FLAG-cGAS E225A/D227A

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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p_m16S

Plasmid

#160549

Purpose

Used for mycoplasma detection by qPCR. 16S insert truncated internally from the strain Mycoplasma capricolum subsp. capricolum strain California Kid. ATB: Carbenicilline

Depositor

Denis Gerlier

Article

Jean et al PLoS One. 2017 Feb 22;12(2):e0172358. do

Insert

16S rDNA fragment from M. capricolum subsp. capricolum strain California Kid (gi_83283139)

Use

Unspecified

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pPalmitoyl-mTurquoise2

Plasmid

#36209

Purpose

In vivo visualization of the membrane (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

p63(1-29) (ARHGEF25 Human)

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pRetroX-PTuner DD-linker-M.EcoGII

Plasmid

#122082

Purpose

Express M.EcoGII with detabilization domain - inducible

Depositor

Laure Crabbe

Article

Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

Insert

DD-linker-M.EcoGII

Use

Retroviral

Tags

V5 tag on Nter of M.EcoGII

Expression

Bacterial and Mammalian

Available Since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pENN239_CRISPRcharm_Kv1

Plasmid

#220838

Purpose

CRISPRcharm Kv1 expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

CRISPRcharm Kv1

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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