163,606 results

1. pTRE3G-human cGAS-HA

   Plasmid

   #186842

   Purpose

   Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   Human cGAS with C-terminal HA tag (Adamts1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   TRE3G

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pV1393:SpRY

   Plasmid

   #172189

   Purpose

   Genome editing of C. albicans

   Depositor

   Doug Bernstein

   Article

   Evans et al mSphere. 2021 May 19;6(3). pii: 6/3/e003

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pV1393

   Plasmid

   #111430

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDZ149

   Plasmid

   #41951

   Depositor

   Stephen Elledge

   Article

   Zhang et al Cell. 2006 Aug 11;126(3):529-42.

   Insert

   ubiquitin specific peptidase 28 (USP28 )
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   C171A

   Promoter

   Available Since

   April 17, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pTRE3G-mouse cGAS-HA

   Plasmid

   #186875

   Purpose

   Doxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   Mouse cGAS with C-terminal HA tag (Cgas Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   TRE3G

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Active Wnt3-V5

   Plasmid

   #43811

   Depositor

   Xi He

   Article

   MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

   Insert

   WNT3 (WNT3 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pECNUS4

   Plasmid

   #184887

   Purpose

   ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

   Depositor

   Eunyoung Chae

   Article

   Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   RPS5A

   Available Since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJSK659

   Plasmid

   #205128

   Purpose

   Expression of GFP-SnxA in mammalian cells

   Depositor

   Jason King

   Article

   Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

   Insert

   SnxA
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDx_mScarlet-I3

   Plasmid

   #189757

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet-I3 red fluorescent protein

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet-I3
   

   Use

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV & Rha

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. BCR/ABL P190 transgenic construct

    Plasmid

    #38185

    Depositor

    Nora Heisterkamp

    Article

    Heisterkamp et al Nature. 1990 Mar 15;344(6263):251-3.

    Insert

    BCR/ABL P190 (ABL1, BCR Human)
    

    Use

    Construct for making transgenic mice

    Tags

    Expression

    Mutation

    Promoter

    truncated mouse MT promoter

    Available Since

    Oct. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBTG2

    Plasmid

    #149475

    Purpose

    Broad-Host range expression vector, pBBR origin, TetR repressor, PTetA promoter, GFPmut3b, Kanamycin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    TetR-GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PTetA

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJUMP25-1A(sfGFP)

    Plasmid

    #126972

    Purpose

    Level 1 vector. sfGFP reporter. Origin RSF1010 (Broad-host-range)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-CMV-Galpha-13 ONE-GO

    Plasmid

    #189740

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Inserts

    Galpha-13 ONE-GO
    GNA13 (GNA13 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    L342M (present in YFP but no functional consequen…

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pcDNA3.1 FLAG-YY1

    Plasmid

    #104396

    Purpose

    FLAG-YY1

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    YY1
    

    Use

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMALcPP-MS2cp

    Plasmid

    #203351

    Purpose

    Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria

    Depositor

    Thomas Cech

    Article

    Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;12

    Insert

    MS2 coat protein
    

    Use

    Tags

    Hx6-MBP

    Expression

    Bacterial

    Mutation

    V75E and A81G, to prevent capsid assembly

    Promoter

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. mApple-Rab5a-7

    Plasmid

    #54944

    Purpose

    Localization: Ras GTPase, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    Rab5a
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pQLink-GST-10xCTD

    Plasmid

    #138472

    Purpose

    Expresses GST-tagged 10xCTD heptapeptide repeat in bacteria

    Depositor

    Britt Glaunsinger

    Article

    Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

    Insert

    Rpb1 (POLR2A Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    10x CTD repeat

    Promoter

    T5

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. PGK-DTAG-CTERM-GFP

    Plasmid

    #185770

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    William Sellers, Alessandra Ianari

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, dTAG

    Expression

    Mutation

    none

    Promoter

    PGK

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. sgGFP_3

    Plasmid

    #78165

    Purpose

    sgGFP

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

20. tetO-TCF21

    Plasmid

    #170706

    Purpose

    doxycycline-inducible overexpression of TCF21

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    TCF21 (TCF21 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TFORF1332

    Plasmid

    #143790

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TFAP2E (TFAP2E Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSpyC-GFP

    Plasmid

    #220191

    Purpose

    sgRNA expression vector - pBG42 promoter with GFP in sgRNA site

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG42 promoter with GFP in sgRNA site
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    monomeric superfolder green fluorescent protein

    Promoter

    pBG42

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMRS-LacI-dCas9

    Plasmid

    #220194

    Purpose

    mini-Tn7 dCas9 expression vector - LacI/pLac promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    LacI/pLac

    Available Since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHR_UAS-tTS-P2A-GFP_PGK-BFP

    Plasmid

    #133808

    Purpose

    inducible expression of tTS and GFP with BFP marker in mammalian cells

    Depositor

    Wendell Lim

    Article

    Toda et al Science. 2018 Jul 13;361(6398):156-162.

    Insert

    tTS-P2A-EGFP-PGK-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. TFORF3367

    Plasmid

    #144843

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ZNF281 (ZNF281 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDZ148

    Plasmid

    #41950

    Depositor

    Stephen Elledge

    Article

    Zhang et al Cell. 2006 Aug 11;126(3):529-42.

    Insert

    ubiquitin specific peptidase 28 (USP28 )
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 17, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pRSET mNG-GECO1

    Plasmid

    #201933

    Purpose

    Expresses mNG-GECO1 indicator in bacteria

    Depositor

    Robert Campbell

    Article

    Zarowny et al ACS Sens. 2020 Jul 24;5(7):1959-1968. do

    Insert

    mNeonGreenGECO1
    

    Use

    Tags

    HIS tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLKO.1-shLacZ-1735

    Plasmid

    #115454

    Purpose

    Constitutive lentiviral expression

    Depositor

    William Hahn

    Article

    Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

    Insert

    shLacZ-1735
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. cfSGFP2-col4a1

    Plasmid

    #160317

    Purpose

    cfSGFP2-tagged collagen4a1 for mammalian expression

    Depositor

    Ikuo Wada

    Article

    Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

    Insert

    COL4A1 (COL4A1 Human)
    

    Use

    Tags

    cfSGFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBS-U6-crRNA-empty

    Plasmid

    #134921

    Purpose

    Components for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.

    Depositor

    Tomoji Mashimo

    Article

    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

    Insert

    crRNA for CRISPR-Cas3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge