We narrowed to 34,443 results for: CaS;

1. YL-CashGem-BAT1-URA3-LINEAR

   Plasmid

   #174837

   Purpose

   pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   Codon optimized Cas9:hGem
   

   Use

   CRISPR and Synthetic Biology

   Tags

   hGeminin tag

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   GPDp

   Available since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMS23: pHelper(PmcCAST)_entry_ΔTnsD

   Plasmid

   #168156

   Purpose

   Inducible expression of PmcCAST proteins (except TnsD). Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   PmcCAST minimal CRISPR array
   PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   PmcCAST Tns proteins (TnsAB and TnsC)
   PmcTniQ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330-NLC-DHFR-SpCas9

   Plasmid

   #124523

   Purpose

   Expresses SpCas9 fused to DHFR domains on both the N- and C- termini and an internal loop in mammalian cells

   Depositor

   Amit Choudhary

   Article

   Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

   Insert

   NLC-DHFR-SpCas9
   

   Use

   AAV

   Tags

   Destabilized domain of E.coli dihydrofolate reduc…

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. peft-3::cas-9::tbb-2 3'UTR

   Plasmid

   #48960

   Purpose

   this plasmid contains the codon optimized gene encoding Cas9 for C. elegans.

   Depositor

   Mario de Bono

   Article

   Chen et al Nucleic Acids Res. 2013 Sep 5.

   Insert

   cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   eef-1A.1 (eft-3)

   Available since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. p2CT-His-MBP-Lne_Cas13a_WT

   Plasmid

   #91866

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LneCas13a (LneC2c2) WT
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX396 Parvibaculum lavamentivorans Cas9

   Plasmid

   #68333

   Purpose

   Parvibaculum lavamentivorans Cas9

   Depositor

   Feng Zhang

   Insert

   PlCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-hCas9-T2A-miRFP670

   Plasmid

   #187223

   Purpose

   Expresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Insert

   Cas9
   

   Use

   Tags

   Inserted T2A-miRFP670 at 3' terminal of MCS.

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET23b-Casp9 C287A-His

   Plasmid

   #11830

   Depositor

   Guy Salvesen

   Article

   Stennicke et al J Biol Chem. 1999 Mar 26. 274(13):8359-6

   Insert

   Caspase 9 catalytic mutant (CASP9 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   C287A

   Promoter

   Available since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pCaSpeR4 mito roGFP2-Orp1

   Plasmid

   #65001

   Purpose

   Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoter

   Depositor

   Tobias Dick

   Article

   Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

   Insert

   Orp1 (HYR1 Budding Yeast)
   

   Use

   Tags

   roGFP2

   Expression

   Insect

   Mutation

   mitochondrial targeting sequence

   Promoter

   Available since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRTS-GBP-dCas9-mRFP

    Plasmid

    #102934

    Purpose

    Tetracyclin inducible expression construct for dCas9 fused to GFP-binding Protein (N-terminally) and mRFP (C-terminus)

    Depositor

    Sebastian Bultmann

    Article

    Anton et al Nucleus. 2017 May 4;8(3):279-286. doi: 1

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    GBP (GFP binding protein and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

11. pX459-HypaCas9-mR2-ACTB_sgRNA

    Plasmid

    #183884

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ACTB sgRNA spacer (ACTB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX459-HypaCas9-mR2-TUBA1B_sgRNA

    Plasmid

    #183888

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    TUBA1B sgRNA spacer (TUBA1B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459-HypaCas9-mR2-RHOA_sgRNA

    Plasmid

    #183877

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    RHOA sgRNA spacer (RHOA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. GCN5 FL-dCas9-CBP core

    Plasmid

    #179556

    Purpose

    encodes S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with n-terminal fusion of full length human GCN5 and c-terminal fusion of human CBP core (aa 1084-1701) (CREBBP, KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX377 Corynebacter diphtheriae Cas9

    Plasmid

    #68314

    Purpose

    Corynebacter diphtheriae Cas9

    Depositor

    Feng Zhang

    Insert

    CdCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHL-EF1a-SphcCas9(D10A)-iP-A

    Plasmid

    #60600

    Purpose

    Expresses D10A mutant (nickase) of human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Inserts

    CRISPR Cas9 D10A
    puromycin resistance gene
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Changed Asp 10 to Ala, Codon usage optimized for …

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMS21: pHelper(PmcCAST)_entry

    Plasmid

    #168154

    Purpose

    Inducible expression of PmcCAST proteins. Two BsaI sites for spacer cloning.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    PmcCAST minimal CRISPR array
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
    PmcTniQ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMMACE DEST CMV SpCas9

    Plasmid

    #206268

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    SpCas9
    

    Use

    CRISPR; Recombinant baculovirus production

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. 2xNLS-NLP-cMyc LbaCas12a

    Plasmid

    #182123

    Purpose

    pET21a protein expression vector for 2xNLS-NLP-cMyc LbaCas12a in bacteria

    Depositor

    Scot Wolfe

    Article

    Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

    Insert

    2xNLS-NLP-cMyc LbaCas12a
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMS3: pHelper(AvCAST)_AvPSP1_ΔTnsD

    Plasmid

    #168135

    Purpose

    Inducible expression of AvCAST proteins (except TnsD) with crRNA targeting AvPSP1.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Inserts

    AvCAST minimal CRISPR array (with spacer for AvPSP1)
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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