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pAAV_hsyn_NES-his-CaMPARI2-F391W-L398V

Plasmid

#119723

Purpose

AAV vector expressing CaMPARI2_F391W-L398V (Kd = 174nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Thomas Oertner

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Inserts

CaMPARI2
CaMPARI2

Use

AAV

Tags

NES-his

Expression

Mammalian

Mutation

F391W, L398V

Promoter

hSyn1

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO-3xFLAG-His6-LARP1

Plasmid

#233626

Purpose

Expression of Flag- and His-tagged LARP1

Depositor

Shintaro Iwasaki

Article

Shichino et al Nat Struct Mol Biol. 2024 Oct;31(10):155

Insert

LARP1 (LARP1 Human)

Tags

3FLAG-His6

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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10xHis-3C-BRD4(42-459) BD1-BD2

Plasmid

#233886

Purpose

pRSF-DUET1 for protein overexpression of human BRD4

Depositor

Alessio Ciulli

Article

Hsia et al Nature. 2024 Mar;627(8002):204-211. doi:

Insert

BRD4 (BRD4 Human)

Tags

10x his 3C

Expression

Bacterial

Promoter

T7

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-Taq DNA Ligase

Plasmid

#178798

Purpose

Inducible expression of Taq DNA Ligase in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Ligase

Expression

Bacterial

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET24a His6 GST PYL2(14-188)

Plasmid

#177842

Purpose

Bacterial Expression of PYL2

Depositor

Karsten Melcher

Article

Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

Insert

PYR1-like 2 (PYL2 Mustard Weed)

Tags

6xHis-GST

Expression

Bacterial

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase

Plasmid

#223747

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

Lambda-PPase

Tags

6xHis-TEV

Expression

Bacterial

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pET-26b-NbVHH05-ALFA-His

Plasmid

#171569

Purpose

Bacterial expression of ALFA-His-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-ALFA-His)

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

NbVHH05-ALFA-His (UBE2J1 Human)

Tags

ALFA-tag/His-tag and PelB signal peptide

Expression

Bacterial

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

Plasmid

#196185

Purpose

Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His

Depositor

Jesse Zalatan

Article

Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

Inserts

GSK3B (GSK3B Human)
λPPase

Tags

GST, HA, His6, and MBP

Expression

Bacterial

Mutation

changed Serine 9 to Alanine

Promoter

Tac

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP

Plasmid

#154303

Purpose

Expresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Inserts

histone H1
γ-tubulin

Use

Dictyostelium expression

Tags

mEGFP and mRuby3

Promoter

actin15

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pTWIN1-His6-Ssp-Httex1-23Q

Plasmid

#84349

Purpose

Expression of the human Huntingtin Exon1 protein containing 23Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon 1 (HTT Human)

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Promoter

T7

Available Since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-Taq DNA Pol

Plasmid

#178782

Purpose

Inducible expression of Taq DNA polymerase in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Polymerase

Expression

Bacterial

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pRS306-URA3-PHIS3-AqLumSynth –Sapphire

Plasmid

#116931

Purpose

Yeast integrative vector for the exression of the 20nm GEM under the HIS3 promoter

Depositor

Liam Holt

Article

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

lumazine synthase

Tags

Sapphire

Expression

Yeast

Promoter

HIS3

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGS-21a his6-GST-WTPhi29DNAP

Plasmid

#166863

Purpose

To produce a dual affinity-tagged Phi29 DNA polymerase

Depositor

Paul Blainey, Stephen Quake

Article

Blainey et al Nucleic Acids Res. 2011 Mar;39(4):e19. d

Insert

Phi29 Polymerase codon-optimized for overexpression in E. coli

Tags

GST and his6

Expression

Bacterial

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pET-(-30)GFP-9xGGS-Cre-6xHis

Plasmid

#62372

Purpose

Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

(-30)GFP-9xGGS-Cre-6xHis

Tags

(-30)GFP and 6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pET11-His6-Pif6-sfGFP-ssrA

Plasmid

#140891

Purpose

Expression of PIF-sfGFP-ssrA in E. coli

Depositor

Neal Devaraj

Article

Bhattacharya et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

PIF-sfGFP-ssrA

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA4-Myc-HisA-H248Y-Sirt3

Plasmid

#24917

Depositor

Toren Finkel

Article

Ahn et al Proc Natl Acad Sci U S A. 2008 Sep 23. 1

Insert

Sirt3 (SIRT3 Human)

Tags

His and Myc

Expression

Mammalian

Mutation

His248 mutated to Tyr(Y)

Available Since

June 10, 2010

Availability

Academic Institutions and Nonprofits only
pFA6a-6xGLY-His-tag-hphMX4

Plasmid

#20764

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available Since

March 27, 2009

Availability

Academic Institutions and Nonprofits only

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Sfp pet29b C-terminal His Tag

Plasmid

#75015

Purpose

Expresses the PPTase Sfp from B. subtilis; loads CoA analogues onto ACP

Depositor

Michael Burkart

Article

Worthington et al Org Biomol Chem. 2006 Jan 7;4(1):44-6. E

Insert

Sfp

Tags

His Tag

Expression

Bacterial

Promoter

T7

Available Since

May 21, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_3C-Avi-His6

Plasmid

#113887

Purpose

Mammalian (inducible) protein expression, in vivo biotinylation

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Type

Empty backbone

Use

Lentiviral

Tags

3C-Avi-His6

Promoter

CMV-MIE-TetO2

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_hsyn_NES-his-CaMPARI2-WPRE-SV40

Plasmid

#101060

Purpose

AAV vector expressing CaMPARI2 (Kd = 200nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Article

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has Service

AAV1

Insert

CaMPARI2

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available Since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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