19,634 results

1. pCDH-CMV-mCherry-T2A-Puro

   Plasmid

   #72264

   Purpose

   Express mCherry and puromycin resistance gene under CMV promoter. 3rd gen lentiviral vector.

   Depositor

   Kazuhiro Oka

   Insert

   mCherry-T2A-Puro
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST17-UbE2S

   Plasmid

   #15807

   Depositor

   Wade Harper

   Article

   Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

   Insert

   Ubiquitin conjugating enzyme UbE2S (UBE2S Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 28, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDF-1b-RFC140ΔN555

   Plasmid

   #175043

   Purpose

   Overexpression of human (Rfc1) p140ΔN555 in E.coli

   Depositor

   Brian Kelch

   Article

   Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   Replication factor C subunit 1 (RFC1 Human)
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. p36-p37-p38-p40-pET-Duet-1

   Plasmid

   #175049

   Purpose

   Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli

   Depositor

   Brian Kelch

   Article

   Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

   Insert

   Replication factor C subunits 2-5 (RFC4, RFC2, RFC3, RFC5 Human)
   

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Red cGull

   Plasmid

   #191853

   Purpose

   Expresses biosensor for cGMP in mammalian cells

   Depositor

   Takashi Tsuboi

   Article

   Takizawa et al Commun Biol. 2022 Sep 5;5(1):833. doi: 1

   Insert

   Red cGull
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-AAV-syn-iGABASnFR2-WPRE

   Plasmid

   #218874

   Purpose

   AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Has Service

   AAV1 and AAV5

   Insert

   iGABASnFR2
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S99A F102Y F104Y L178S

   Promoter

   Synapsin

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. p5154

   Plasmid

   #175390

   Purpose

   High Copy Number DNA Donor Vector for Bxb1 Integrase-mediated Cassette Exchange (for inserts <15kb) into dual site mouse strains (Bxb-GT/GA)

   Depositor

   Michael Wiles

   Article

   Low et al Sci Rep. 2022 Mar 31;12(1):5424. doi: 10

   Type

   Empty backbone

   Use

   Mouse Targeting; Cloning vector, to be used to de…

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-LC3

   Plasmid

   #21073

   Purpose

   Mammalian expression of rat LC3 fused to EGFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   First ATG is not included.

   Promoter

   CMV

   Available Since

   May 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSIP403

   Plasmid

   #122028

   Purpose

   Plasmid for expression of gene of interest in Lactobacillus plantarum and L. sakei

   Depositor

   Geir Mathiesen, Lars Axelsson

   Article

   Sorvig et al Microbiology. 2005 Jul;151(Pt 7):2439-49

   Insert

   gusA
   

   Expression

   Bacterial

   Promoter

   sppA

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-SFFV-KRAB-dCas9-P2A-mCherry

    Plasmid

    #60954

    Purpose

    2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

    Depositor

    Jonathan Weissman

    Article

    Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

    Insert

    KRAB-dCas9-P2A-mCherry fusion
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA, KRAB domain, and mCherry

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. WT Smarca4-sfGFP

    Plasmid

    #107056

    Purpose

    Expression of wild-type Smarca4 (Brg1) with C-terminal super-folder GFP fusion.

    Depositor

    Courtney Hodges, Jerry Crabtree

    Article

    Hodges et al Nat Struct Mol Biol. 2018 Jan;25(1):61-7

    Insert

    SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (Smarca4 Mouse)
    

    Use

    Lentiviral

    Tags

    Super-folder GFP (sfGFP)

    Expression

    Mammalian

    Mutation

    wild-type

    Promoter

    CAG

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti SpBsmBI sgRNA Hygro

    Plasmid

    #62205

    Purpose

    An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

    Depositor

    Rene Maehr

    Article

    Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTP396

    Plasmid

    #149336

    Purpose

    Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

    Depositor

    Rebecca Voorhees

    Article

    Pleiner et al Science. 2020 May 21. pii: science.abb50

    Insert

    anti-GFP nanobody
    

    Tags

    Biotin acceptor peptide (Avi-tag), His14, and SUM…

    Expression

    Bacterial

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn1-FLEX-CybGam-WPRE

    Plasmid

    #207662

    Purpose

    Control for CybSEP2

    Depositor

    Sung Han

    Article

    Kim et al bioRxiv 2023.01.19.524797

    Insert

    Gamillus
    

    Use

    AAV

    Mutation

    Histidine 86 and 159 to alanines, inserted gamill…

    Promoter

    hSynapsin

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLKO.1 neo

    Plasmid

    #13425

    Purpose

    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses neomycin for selection.

    Depositor

    Sheila Stewart

    Insert

    non-hairpin 18bp
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Dec. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-Rab27A

    Plasmid

    #89237

    Purpose

    Expression of GFP-tagged human Rab27A in mammalian cells

    Depositor

    William Gahl

    Article

    Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

    Insert

    human Rab27A (RAB7A Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available Since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVX-EF1alpha-eGFP-2xStrep-IRES-Puro

    Plasmid

    #141395

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCherry-C1-RanQ69L

    Plasmid

    #30309

    Depositor

    Jay Brenman

    Article

    Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

    Insert

    RanQ69L (RAN N/A)
    

    Tags

    FLAG-Cherry

    Expression

    Mammalian

    Mutation

    Glutamine 69 is changed to Leucine

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLJM1-Empty

    Plasmid

    #91980

    Purpose

    Empty backbone

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. p4xmts-mScarlet3_N1

    Plasmid

    #189774

    Purpose

    Construct for labelling mitochondria in mammalian cells using mitochondrial targeted mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    4xmts-mScarlet3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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