19,773 results

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lenti_dCas9-KRAB-MeCP2

Plasmid

#122205

Purpose

Bipartite dCas9 repressor (dCas9-KRAB-MeCP2) in a UCOE-SFFV-dCas9 lentiviral vector.

Depositor

Andrea Califano

Article

Tan et al bioRxiv 2021.06.28.449297

Insert

dCas9-KRAB-MeCP2

Use

Lentiviral

Tags

KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 (D10A, H840A)

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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GW1-pHRed

Plasmid

#31473

Purpose

genetically encoded pH sensor made from mutagenesis of the red fluorescent protein mKeima

Depositor

Gary Yellen

Article

Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

Insert

pHRed

Expression

Mammalian

Available Since

July 21, 2011

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro CDK4 R24C

Plasmid

#11254

Depositor

Bob Weinberg

Article

Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Expression

Mammalian

Mutation

R24C

Available Since

Feb. 14, 2006

Availability

Academic Institutions and Nonprofits only

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EBFP2-H2B-6

Plasmid

#55243

Purpose

Localization: Nucleus/Histones, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Tags

EBFP2

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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MaLionG

Plasmid

#113906

Purpose

Green ATP sensor

Depositor

Tetsuya Kitaguchi

Article

Arai et al Angew Chem Int Ed Engl. 2018 Jun 27. doi

Insert

MaLionG

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pBiex-1 GFPNanobody_SNAP

Plasmid

#82711

Purpose

Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

Insert

GFP Nanobody

Tags

6xHis Purification tag, FLAG purification tag, an…

Expression

Bacterial and Insect

Promoter

T7 Promoter

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-LC3

Plasmid

#21073

Purpose

Mammalian expression of rat LC3 fused to EGFP

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

EGFP

Expression

Mammalian

Mutation

First ATG is not included.

Promoter

CMV

Available Since

May 11, 2009

Availability

Academic Institutions and Nonprofits only

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pLNCX2-CFP-VGPDFGR-YFP

Plasmid

#59587

Purpose

Encodes a granzyme B FRET reporter

Depositor

Tim Mitchison

Article

Choi et al Proc Natl Acad Sci U S A. 2013 Apr 16;11

Insert

ECFP-VGPDFGR-Venus

Use

Retroviral

Tags

ECFP and Venus

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pTP264

Plasmid

#149334

Purpose

Bacterial expression of Biotin ligase BirA

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Biotin ligase BirA

Tags

His14 and bdNEDD8

Expression

Bacterial

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-MCS-WPRE-pA

Plasmid

#63931

Purpose

Empty AAV-RAM plasmid w. multiple cloning site (MCS)

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Type

Empty backbone

Use

AAV

Promoter

pRAM and TRE

Available Since

Dec. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDIRECT_22A

Plasmid

#91133

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:AtCas9 + AtU6:gRNA

Use

CRISPR

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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G6PD/pRK5

Plasmid

#41521

Depositor

Xiaolu Yang

Article

Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

Insert

glucose-6-phosphate dehydrogenase (G6PD Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV5B-Flag-Smurf2 wt

Plasmid

#11746

Depositor

Jeff Wrana

Article

Kavsak et al Mol Cell. 2000 Dec . 6(6):1365-75.

Insert

SMURF2 (SMURF2 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-Ef1a-DIO eNpHR 3.0-EYFP

Plasmid

#26966

Purpose

AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

Depositor

Karl Deisseroth

Article

Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

eNpHR 3.0

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

ER export at 3' end of gene. Trafficking sig…

Promoter

EF1alpha

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only

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PER2:Luc Plasmid

Plasmid

#206875

Purpose

Per2 promoter luciferase reporter

Depositor

Michelle Farkas

Article

Lin et al Integr Cancer Ther. 2019 Jan-Dec;18:1534

Insert

Period 2 (Per2 Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-Gal4ff.zf1

Plasmid

#61392

Purpose

mRNA synthesis of Gal4ff codon modified for zebrafish

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Insert

Gal4ff.zf1

Use

Zebrafish expression

Promoter

sp6

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pMK1334

Plasmid

#127965

Purpose

sgRNA expression vector compatible with CROP-Seq and imaging screens

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

EF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pBPZpGAL4DBDUw

Plasmid

#26233

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

Zip-GAL4DBD

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR221_EGFP

Plasmid

#25899

Purpose

Gateway entry vector containing EGFP.

Depositor

David Root

Article

Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

Insert

EGFP

Use

Gateway entry vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ArchT-BFP2-TSERex

Plasmid

#123312

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

ArchT-BFP2

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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