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9,148 results

Showing: 3021 - 3040 of 9148 results

  1. pJR85

    Plasmid
    #140095
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.
    Depositor
    Jonathan Weissman
    Publication
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    140095-plasmid-map-sequence-id-292013

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  7. 4xNFkB Luc

    Plasmid
    #111216
    Purpose
    reporter gene
    Depositor
    Johannes A. Schmid
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    firefly Luciferase
    Expression
    Mammalian
    Available since
    Nov. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    111216-plasmid-map-sequence-id-213082

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  10. pC0040-LwaCas13a crRNA backbone

    Plasmid
    #103851
    Purpose
    Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA
    Depositor
    Feng Zhang
    Publication
    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    U6-LwaCas13aDR-BbsI-BbsI-polyT
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Nov. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    103851-plasmid-map-sequence-id-199180

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  11. pBa-LSS-GFP-LDLR wt

    Plasmid
    #98184
    Purpose
    Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.
    Depositor
    Gary Banker, Marvin Bentley
    Publication
    Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LDLR (LDLR Human)
    Tags
    GFP and LDLR signal sequence, N-term of GFP so GF…
    Expression
    Mammalian
    Mutation
    none
    Promoter
    Chicken Beta Actin
    Available since
    Sept. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    98184-plasmid-map-sequence-id-490370

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  13. pAAVS1-NC-CRISPRi (Gen3)

    Plasmid
    #73499
    Purpose
    Constitutive CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus
    Depositor
    Bruce Conklin
    Publication
    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-KRAB
    Use
    CRISPR
    Tags
    HA, KRAB, and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically deactivated Cas9)
    Promoter
    CAG
    Available since
    Dec. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    73499-plasmid-map-sequence-id-364405

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  18. Tet-inducible Luciferase reporter

    Plasmid
    #64127
    Purpose
    Photoactivatable transcription system. Luciferase reporter under the control of a tet-inducible promoter.
    Depositor
    Moritoshi Sato
    Publication
    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    luciferase
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    TetOx13-CMV
    Available since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    64127-plasmid-map-sequence-id-198432

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  20. pFLAG-CMV-hErk1

    Plasmid
    #49328
    Purpose
    Expresses FLAG-tagged human Erk1
    Depositor
    Melanie Cobb
    Insert
    Erk1 (MAPK3 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    49328-plasmid-map-sequence-id-148204

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Showing: 3021 - 3040 of 9148 results