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Showing: 6041 - 6080 of 10096 results

  11. pHR-UCOE-SFFV-dCas9-P2A-EGFP

    Plasmid
    #188898
    Purpose
    Human lentiviral vector for expression of dCas9 with a C-terminal HA-2xNLS and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element
    Depositor
    Marco Jost, Jonathan Weissman
    Article
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    dCas9
    Use
    Lentiviral
    Tags
    HA-2xNLS and P2A-GFP
    Expression
    Mutation
    Promoter
    SFFV
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    188898-plasmid-map-sequence-id-374870

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  36. pBridge-tyrosinase.σ2

    Plasmid
    #197415
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-2 σ2
    Use
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    encodes R42G substitution, contains silent substi…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197415-plasmid-map-sequence-id-388893

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Showing: 6041 - 6080 of 10096 results