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Addgene
Showing: 4561 - 4590 of 157662 results
  1. pMKO.1 GFP

    Plasmid
    #10676
    Purpose
    Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.
    Depositor
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    RNAi and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 7, 2005
    Availability
    Academic Institutions and Nonprofits only
  2. Sema6b(S)-AP-His

    Plasmid
    #72040
    Purpose
    Expresses the extracellular region of the Sema6B protein (truncated at extracellular domain cleavage site; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.
    Depositor
    Insert
    Sema6b (Sema6b Mouse)
    Use
    Tags
    AP-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
  3. pdCas9-tdTomato-VPR

    Plasmid
    #232632
    Purpose
    VPR activation domain coupled to catalytically inactive dCas9 for localization; contains tdTomato for labeling and as a spacer
    Depositor
    Insert
    dCas9-tdTomato-VPR
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Feb. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pCHW111101

    Plasmid
    #222452
    Purpose
    Level 1, Position 7 containing CBS4-miniDFR::Cre::NOS.
    Depositor
    Insert
    CBS4-miniDFR::Cre::NOS
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    no
    Promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. GPER-DuET

    Plasmid
    #213256
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Insert
    GPER (GPER1 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pCHAP3_Sap

    Plasmid
    #206849
    Purpose
    A plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was domesticated for SapI and integrated into the chloroplast fragment.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. pCHAP2

    Plasmid
    #206847
    Purpose
    A plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 4561 - 4590 of 157662 results