165,971 results

1. TFORF2061

   Plasmid

   #141900

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST exorh:mCherry

   Plasmid

   #195989

   Purpose

   Transgenesis backbone

   Depositor

   Christian Mosimann, Nicolas Rohner

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Other

   Available Since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57-MCS1-2A-MCS2

   Plasmid

   #71782

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Expression

   Mammalian

   Available Since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3 HA-Sumo1 WT

   Plasmid

   #48966

   Purpose

   Expresses HA-Sumo1 WT in mammalian cells

   Depositor

   Guy Salvesen

   Article

   Bekes et al J Biol Chem. 2011 Mar 25;286(12):10238-4

   Insert

   SUMO1 (SUMO1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Oct. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221_SLC9A9

   Plasmid

   #132211

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC9A9 (SLC9A9 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 28, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. AAV2-ITR-pEFS-NLS_hfCas13d(RfxCas13d_N2V8)_FLAG_NLS-pA-U6-DR-Pcsk9-sg5-DR-Pcsk9-sg6-DR-AAV2-ITR

   Plasmid

   #191795

   Purpose

   vector for encoding and AAV packaging a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by EFS promoter, Pcsk9-target guide RNAs compatible with RfxCas13d driven by hU6

   Depositor

   Huawei Tong

   Article

   Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

   Insert

   humanized hfCas13d
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Mutation

   A134V, A140V, A141V, A143V

   Promoter

   EFS, hU6

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-3XHA-p53

   Plasmid

   #196267

   Purpose

   Mammalian expression of 3xHA tagged wild-type p53

   Depositor

   Laura Attardi

   Article

   Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

   Insert

   p53 (Trp53 Mouse)
   

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. RMCE wild type DNA-PKcs

   Plasmid

   #233911

   Purpose

   Low copy number provides expression of human DNA-PKcs

   Depositor

   Katheryn Meek

   Article

   Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

   Insert

   dna-pkcs cDNA (PRKDC Human)
   

   Expression

   Mammalian

   Available Since

   April 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGreen2x35SLUC

   Plasmid

   #108228

   Purpose

   Plant expression as translational fusions to FLUC with RLUC normalization

   Depositor

   Alejandro Ferrando

   Article

   Solé-Gil et al Methods Mol Biol. 2024;2722:79-87. doi:

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Firefly Luciferase

   Expression

   Plant

   Promoter

   2x35S

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR4_G3BP2

    Plasmid

    #127105

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    G3BP2 (G3BP2 Human)
    

    Use

    Gateway shuttling vector

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-PGK-EGFP-ALDH1A3-Neo

    Plasmid

    #189744

    Purpose

    The construct was used to express GFP tagged human ALDH1A3 in mammalian cells for the analysis of the subcellular location of this protein.

    Depositor

    Robert Sobol

    Article

    Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

    Insert

    ALDH1A3 (ALDH1A3 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Mito-PyronicSF/pCMV-myc-mito

    Plasmid

    #124813

    Purpose

    Highly resposive GFP-based pyruvate nanosensor to explore mitochondrial metabolism.

    Depositor

    Luis Felipe Barros

    Article

    Arce-Molina et al eLife. 2020 Mar 6;9. pii: 53917. doi: 10

    Insert

    PyronicSF
    

    Tags

    Mitochondrial Sequence Signal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Integrin beta 2 - mYFP

    Plasmid

    #8638

    Depositor

    Timothy Springer

    Article

    Kim et al Science 2003 Sep 19;301(5640):1720-5.

    Insert

    Integrin beta 2 (ITGB2 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    stop codon of beta2 was replaced with AgeI restri…

    Available Since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKT0178

    Plasmid

    #8744

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    tdimer2
    

    Tags

    Codon optimized linker

    Expression

    Yeast

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG416Gal-GFP

    Plasmid

    #38309

    Depositor

    Susan Lindquist

    Article

    McLellan et al ACS Chem Biol. 2012 Jun 22.

    Insert

    EGFP (eGFP )
    

    Expression

    Yeast

    Available Since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

16. RMCE halo-flag DNA-PKcs

    Plasmid

    #233918

    Purpose

    Low copy number provides expression of human DNA-PKcs with an N-terminal halo-flag tag.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs (PRKDC Human)
    

    Tags

    flag-halo

    Expression

    Mammalian

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. UBC1cer120G

    Plasmid

    #161017

    Purpose

    ubiquitously weakly expressed 120-meric GFP nanocage

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    120meric GFP nanocage
    

    Expression

    Plant

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLminP_Luc2P_RE4

    Plasmid

    #90338

    Purpose

    NRF2 - Antioxidant response element gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    NRF2

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. Integrin β4-GFP

    Plasmid

    #205092

    Purpose

    Express GFP-tagged human integrin β4 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 4 (ITGB4 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAG416GAL-ccdB-EYFP

    Plasmid

    #14219

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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21. pFUW-tetO-Irf4

    Plasmid

    #139804

    Purpose

    doxycycline-inducible expression of mouse Irf4 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Irf4 (Irf4 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pMAT11

    Plasmid

    #112592

    Purpose

    Used for expression of protein in E. coli as Thrombin and TEV cleavableN-terminal 6His-tag-MBP fusions and without NcoI site within MBP.

    Depositor

    Marko Hyvönen, Johan Peränen

    Article

    Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

    Type

    Empty backbone

    Tags

    6His-MBP

    Expression

    Bacterial

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAAV-CAG-smURFP-P2A-GFP

    Plasmid

    #197151

    Purpose

    Expresses the protein of smURFP-P2A-GFP in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

    Insert

    smURFP-P2A-GFP
    

    Use

    AAV

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. ABE8e-SpRY

    Plasmid

    #226858

    Purpose

    Expresses ABE8e-SpRY base editor in mammalian cells with eGFP marker

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    ABE8e-SpRY
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. HSP70-pp7i-mCh-UPG

    Plasmid

    #161011

    Purpose

    C-terminal mCherry fusion to Arabidopsis HSP70 with intronic PP7

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    PP7 and H2B-mScarlet
    

    Expression

    Plant

    Available Since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. HDAC3 Flag

    Plasmid

    #13819

    Purpose

    Mammalian expression of human histone deacetylase 3 with flag tag

    Depositor

    Eric Verdin

    Article

    Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

    Insert

    HDAC3 (HDAC3 Human)
    

    Tags

    flag

    Expression

    Mammalian

    Available Since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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27. iLOV-Farnesyl-5

    Plasmid

    #56516

    Purpose

    Localization: Membrane, Excitation: 450, Emission: 495

    Depositor

    Michael Davidson

    Insert

    Farnesyl (HRAS Human)
    

    Tags

    iLOV

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

28. pAd388-shLuc

    Plasmid

    #83275

    Purpose

    shRNA targeting luciferace

    Depositor

    Joe Landry

    Article

    Mayes et al Oncotarget. 2017 May 12;8(38):64344-6435

    Insert

    shRNA to Luciferase
    

    Use

    Retroviral

    Promoter

    pUC ori

    Available Since

    Dec. 2, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pJC162.1_pc2RNAi

    Plasmid

    #26541

    Depositor

    Phillip Newmark

    Article

    Collins et al PLoS Biol. 2010 . 8(10):e1000509.

    Insert

    prohormone convertase 2
    

    Use

    RNAi

    Available Since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

30. CIRC-V1-Ana-CVB3 IRES-EGFP-BsaI

    Plasmid

    #246755

    Purpose

    RNA circularization with CIRC using Ana intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BsaI.

    Depositor

    Wensheng Wei

    Article

    Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

    Insert

    CIRC-V1-Ana-CVB3 IRES-EGFP-BsaI
    

    Use

    Synthetic Biology

    Available Since

    Oct. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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