159,332 results

1. EGFP-Tubulin-6

   Plasmid

   #56450

   Purpose

   Localization: Microtubules, Excitation: 488, Emission: 507

   Depositor

   Michael Davidson

   Article

   Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pCSC-LHX8-IRES-GBX1

   Plasmid

   #182235

   Purpose

   To convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with ASCL1, Sox11, FGF2 and two small molecules, forskolin and LDN-193189

   Depositor

   Chun-Li Zhang

   Article

   Ma et al Mol Neurodegener. 2020 Oct 21;15(1):61.

   Insert

   LHX8-IRES-GBX1 (LHX8, GBX1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-mBlueberry2-P2A-EGFP

   Plasmid

   #184937

   Purpose

   Expresses blue fluorescent protein mBlueberry2 and EGFP in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   mBlueberry2-P2A-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Strep-KIFC1*-mCh

   Plasmid

   #120169

   Purpose

   Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member C1 (KIFC1 Human)
   

   Use

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated KIFC1: 125-673 aa

   Promoter

   CMV

   Available since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKb-TnpB2

   Plasmid

   #215334

   Purpose

   ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site

   Depositor

   Kutubuddin Molla

   Article

   Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10

   Insert

   Rice codon optimized ISDra2 TnpB
   

   Use

   Tags

   Not applicable

   Expression

   Plant

   Mutation

   Promoter

   Rice ubiquitin promoter for TnpB and maize ubiqui…

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-sadCas9-VP64

   Plasmid

   #115790

   Purpose

   An AAV vector expressing S. aureus dCas9 fused to VP64.

   Depositor

   Nadav Ahituv

   Article

   Matharu et al Science. 2018 Dec 13. pii: science.aau06

   Insert

   Sa-dCas9
   

   Use

   AAV

   Tags

   NLS and NLS-VP64

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-N-Puro-mNeon-ACTB

   Plasmid

   #207752

   Purpose

   Donor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. ppyCAG_RNaseH1_WT

   Plasmid

   #111906

   Purpose

   Express RNASEH1 in mammalian cell.

   Depositor

   Xiang-Dong Fu

   Article

   Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

   Insert

   RNASEH1 (RNASEH1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-29b(+)_His-CBD-SpEndoH

   Plasmid

   #218196

   Purpose

   Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH

   Depositor

   Florence Pojer

   Insert

   EndoH
   

   Use

   Tags

   tufA-His-CBD

   Expression

   Bacterial

   Mutation

   codon optimized Ecoli, residues 45-313

   Promoter

   T7

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJBL715

    Plasmid

    #140385

    Purpose

    Expresses MphR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-MphR-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV hSyn FLEx-FRT hM4Di-mCherry

    Plasmid

    #161576

    Purpose

    Expression of hM4Di-mCherry in a Flp-dependent fashion

    Depositor

    Robert Malenka, Kevin Beier

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    hM4Di-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    NA

    Promoter

    hSyn

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ecoli cpGFP

    Plasmid

    #215713

    Purpose

    Expresses cpGFP in Ecoli

    Depositor

    Katharine Diehl

    Article

    Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

    Insert

    cpGFP
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    circularly permuted GFP

    Promoter

    T7

    Available since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHCMV_FLUCV_HE

    Plasmid

    #207275

    Purpose

    Mammalian expression of Influenza C virus HE protein

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    FLUCV_HE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSDMA58

    Plasmid

    #67942

    Purpose

    Complementation in Cryptococcus neoformans. Targets DNA constructs to a Safe Haven Site, a small gene-free region. Contains HYG resistance marker.

    Depositor

    James Fraser

    Article

    Arras et al PLoS One. 2015 Apr 9;10(4):e0122916. doi

    Type

    Empty backbone

    Use

    Cryptococcal complementation vector

    Tags

    Expression

    Mutation

    Promoter

    ACT1 for Cryptococcal marker

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGM1202

    Plasmid

    #69615

    Purpose

    inducible gene expression in Streptomyces, C-terminal His-tag encoding sequence

    Depositor

    Günther Muth

    Article

    Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

    Inserts

    aac
    tsr
    rep
    sso
    TipA promoter
    dso
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKM512

    Plasmid

    #140192

    Purpose

    For curing of Orbit-integrated plasmids and associated drug marker

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Inserts

    zeocin resistance gene
    Bxb1gp47 (directionality factor)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cloned in gp47 directinality factor in place of R…

    Promoter

    EM7 and Ptet

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB-cT3G-cERP2-RUNX2

    Plasmid

    #192926

    Purpose

    Barcoded piggybac transposon vector with Dox-inducible expression of RUNX2

    Depositor

    George Church

    Article

    Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

    Insert

    RUNX2 (RUNX2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    TET3G-miniCMV

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. STAT3-GFP

    Plasmid

    #110495

    Purpose

    reporter of STAT3 signaling activity

    Depositor

    Michael Lewis

    Article

    Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

    Insert

    4 repeats of STAT3 binding site M67 at GFP promoter region
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PRK5-FLAG-Bag6

    Plasmid

    #61836

    Purpose

    express N-terminally FLAG tagged human Bag6

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    Bag6 (BAG6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. L0-I35 p35S-XVE-pEstradiol GG0-GG1

    Plasmid

    #195904

    Purpose

    Level 0 inducibile estradiol promter and XVE activator protein (p35S-XVE gene-tRBCS-tNOS-LexA-p35S with GG0 and GG1 golden gate linkers)

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    p35S-XVE-LexA-p35S
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Flag-HA-OTUD5

    Plasmid

    #22610

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    OTUD5 (OTUD5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJMP3665

    Plasmid

    #222351

    Purpose

    Expression vector with PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

    Depositor

    Jason Peters

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Flag-ZDHHC5

    Plasmid

    #231654

    Purpose

    Expresses human ZDHHC5 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC5 (ZDHHC5 Human)
    

    Use

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV- κLight1.3

    Plasmid

    #201223

    Purpose

    Express kLight1.3 biosensor in mammalian cells

    Depositor

    Lin Tian

    Article

    Dong et al Nat Neurosci. 2024 Jul 15. doi: 10.1038/

    Insert

    κLight1.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUC18T-mini-Tn7T-hph-Ptet2

    Plasmid

    #218368

    Purpose

    Empty backbone for Tn7-site insertion with tetR-PtetA expression system with two tetR operator sites

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. [pSK122] pETDuet GST-p18(delta5)

    Plasmid

    #136144

    Purpose

    bacterial expression of GST-p18

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Proc Natl Acad Sci U S A. 2018 Sep 18;11

    Insert

    GST-p18
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRSET Nucleolin

    Plasmid

    #13037

    Depositor

    Peter Newburger

    Article

    Wu et al J Cell Biochem. 2000 Apr . 77(3):507-16.

    Insert

    Nucleolin (NCL Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti-basP:fLuc-TK:rLuc

    Plasmid

    #138369

    Purpose

    Lentiviral plasmid contains basal promoter driving firefly Luciferase 2 and Renilla Luciferase driven by TK promoter

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    sequence multi cloning sites, minP driving firefly luciferase and TK promoter driving renilla luciferase
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p(U6a-BsaI-gRNA)

    Plasmid

    #65955

    Purpose

    Tribolium U6a promoter with BsaI cloning site to insert guide RNA sequence

    Depositor

    Michalis Averof

    Article

    Gilles et al Development. 2015 Jul 9. pii: dev.125054

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1hSIRT5_HA

    Plasmid

    #24483

    Depositor

    Eric Verdin

    Article

    Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

    Insert

    Sirtuin 5 (SIRT5 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge