We narrowed to 17,071 results for: Por

1. GFP-PASS

   Plasmid

   #193970

   Purpose

   Monitoring the production of phosphatidic acid with GFP-PASS (phosphatidic acid biosensor with superior sensitivity)

   Depositor

   Guangwei Du

   Article

   Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

   Insert

   PA biosenor
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV-NINJ1

   Plasmid

   #201179

   Purpose

   Expression of NINJ1 gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Borges et al Elife. 2022 Dec 5;11:e78609. doi: 10.755

   Insert

   Ninjurin 1 (Ninj1 Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Promoter

   MESV

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. CROPseq-tRFP

   Plasmid

   #230938

   Purpose

   Modified version of the original CROP-seq vector (Addgene #86708), expressing tRFP instead of puroR for FACS

   Depositor

   Hyejung Won

   Article

   Lee et al Cell. 2025 Jan 22:S0092-8674(24)01435-1.

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   TagRFP

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTn7CdrA-gfpC

   Plasmid

   #111616

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species.

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Expression

   Bacterial

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. TLCV2-DelGFP

   Plasmid

   #133302

   Purpose

   EGFP reporter was removed in the TLCV2 vector so it could be used for inducible gene CRISPR knockout in cell lines with an eGFP reporter.

   Depositor

   Jingshi Shen

   Article

   Wang et al J Biol Chem. 2019 Dec 27;294(52):19988-1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLNCX2-mEGFP-TSG101

   Plasmid

   #116925

   Purpose

   expresses mEGFP-TSG101 in mammalian cells

   Depositor

   Sanford Simon

   Article

   Bleck et al Proc Natl Acad Sci U S A. 2014 Aug 19;11

   Insert

   TSG101 (TSG101 Human)
   

   Use

   Retroviral

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRluc-LC3wt

   Plasmid

   #105002

   Purpose

   Mammalian expression of Rluc-LC3wt. Can be used for measuring autophagic flux.

   Depositor

   Marja Jããttelã

   Article

   Farkas et al Methods Enzymol. 2017;588:1-13. doi: 10.

   Insert

   LC3B (Map1lc3b Rat)
   

   Use

   Luciferase

   Tags

   Rluc with C124A substitution

   Expression

   Mammalian

   Available Since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEasyCas9

   Plasmid

   #184909

   Purpose

   Expresses the Streptococcus pyogenes Cas9 protein

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. P445-FBP_17

   Plasmid

   #139708

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is flower specific, circadian controlled

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pPhODO1:NnLuz:tAtuOcs
   

   Use

   Luciferase

   Expression

   Plant

   Promoter

   various

   Available Since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. plenti-CMV-mCherry-T2A-GFP

    Plasmid

    #109427

    Purpose

    A lentiviral backbone expressing mCherry-T2A-eGFP off of a CMV promoter.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    mCherry T2A GFP
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-Trak1

    Plasmid

    #127621

    Purpose

    N-terminal GFP-tagged mouse Trak1

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Trak1 (Trak1 Mouse)
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. XLone-ETV2

    Plasmid

    #135763

    Purpose

    expresses human ETV2 upon doxycycline treatment

    Depositor

    Xiaojun Lian

    Article

    Ream et al Stem Cell Reports. 2024 Mar 5:S2213-6711

    Insert

    ETV2 (ETV2 Human)
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    TRE3G

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTriEx-RhoA FLARE.sc Biosensor WT

    Plasmid

    #12150

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor (RHOA Human)
    

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry

    Plasmid

    #124494

    Purpose

    Expresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherry

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Caspase-1 cleavage seq) T2A mCherry
    

    Use

    Synthetic Biology

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV6-AC EJ7-GFP

    Plasmid

    #113617

    Purpose

    Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP

    Depositor

    Jeremy Stark

    Article

    Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

    Insert

    EJ7-GFP variant of EGFP
    

    Expression

    Mammalian

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. Dat - EGFP - Tol2

    Plasmid

    #85418

    Purpose

    for making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) gene

    Depositor

    Marc Ekker

    Article

    Xi et al Dev Dyn. 2011 Nov;240(11):2539-47. doi:

    Insert

    dopamine transporter (slc6a3 Zebrafish)
    

    Use

    Zebrafish transgenesis

    Tags

    EGFP

    Promoter

    dat

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

17. pJAM1.20_PB-NEDD4L_deltaC2-PGK-Blast

    Plasmid

    #205565

    Purpose

    Express human NEDD4L (delta C2 isoform)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    NEDD4L (NEDD4L Human)
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    delta C2 isoform

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAG654 pFAST

    Plasmid

    #172861

    Purpose

    Expresses pFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    pFAST
    

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-sbGLuc-B7-EYFP

    Plasmid

    #114110

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Gomez-Ramirez et al J Neurosci Res. 2020 Mar;98(3):471-480.

    Inserts

    Gaussia luciferase, slow burn
    B7 hinge region
    Enhanced Yellow Fluorescent Protein
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Hy_pMT Flag-URH49 WT

    Plasmid

    #110127

    Purpose

    Expresses Flag-tagged human URH49 (DDX39A)

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    URH49 (DDX39A Human)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    April 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pLexM-SLC7A11_GFP

    Plasmid

    #197995

    Purpose

    Expresses SLC7A11 with an N-terminal FLAG tag and C-terminal TEV-GFP-His8 tag in mammalian cells

    Depositor

    Simon Newstead

    Article

    Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

    Insert

    SLC7A11 (SLC7A11 Human)
    

    Tags

    FLAG tag and TEV-GFP-His8

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pC0061 PsmCas13 (B05) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115211

    Purpose

    Expresses PsmCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    PsmCas13
    

    Tags

    SUMO

    Expression

    Bacterial

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-hRARβ.hRXRα

    Plasmid

    #135415

    Purpose

    Human Retinoic Acid Receptor-beta and Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector

    Depositor

    Catharine Ross

    Article

    Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

    Inserts

    Human retinoic acid receptor-beta
    Human retinoid X receptor-alpha
    

    Expression

    Mammalian

    Promoter

    CMV and SV40

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCHA1.1-MFSD12-V5

    Plasmid

    #149508

    Purpose

    Stable expression of human MFSD12 with recoded nucleotide sequence.

    Depositor

    David Sabatini

    Article

    Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

    Insert

    MFSD12 (MFSD12 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    Codon Optimized Sequence

    Promoter

    Ubc

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pR0306 CcaCas13b His6-TwinStrep-SUMO

    Plasmid

    #182687

    Purpose

    Expresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purification

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    CcaCas13b
    

    Tags

    SUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Circular-oc-CVB3-GFP

    Plasmid

    #226262

    Purpose

    Production of in vitro circularizable RNA via IVT

    Depositor

    Prashant Mali

    Article

    Tong et al Nat Biomed Eng. 2024 Aug 26. doi: 10.103

    Insert

    EGFP
    

    Use

    In vitro transcription

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EGFR-mApple

    Plasmid

    #203773

    Purpose

    Number and brightness analysis of EGFR oligomerization

    Depositor

    Thorsten Wohland

    Article

    Sankaran et al Nat Commun. 2021 Mar 19;12(1):1748. doi:

    Insert

    EGFR (EGFR Human)
    

    Tags

    mApple

    Expression

    Mammalian

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSNK

    Plasmid

    #135193

    Purpose

    Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassette

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    I-SceI lamba Red
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Promoter

    araBAD

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAC-ZEAXipi

    Plasmid

    #53287

    Purpose

    Contains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coli

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    crtE, idi, crtY, crtI, crtB, crtZ
    

    Use

    Low copy number bacterial cloning vector

    Promoter

    endogenous promoters

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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30. EYFP Peroxisome visual barcode

    Plasmid

    #158677

    Purpose

    tagging clonal populations with Peroxisomal EYFP fluorescent protein. Adding Peroxisome localization signal to EYFP WC

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    EYFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pET28-sfGFP[N150TAA]

    Plasmid

    #164581

    Purpose

    superfolder GFP with N150TAA mutation

    Depositor

    Ryan Mehl

    Article

    Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

    Insert

    sfGFP-150TAA
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N150TAA Mutation

    Promoter

    T7

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pEYFP-Sec24C

    Plasmid

    #66608

    Purpose

    YFP fusion of Sec24C

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

    Insert

    Sec24C (SEC24C Human)
    

    Expression

    Mammalian

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLentiPGK Hygro DEST H2B-mCerulean3

    Plasmid

    #90234

    Purpose

    Lentiviral vector to express H2B-mCerulean3 under PGK promoter (With Hygromycin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    H2B
    

    Use

    Lentiviral

    Tags

    mCerulean3

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMXs-puro-RFP-ATG9A

    Plasmid

    #60609

    Purpose

    Expresses RFP-hATG9A in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

    Insert

    Homo sapiens autophagy related 9A (PPARG Human)
    

    Use

    Retroviral

    Tags

    RFP

    Expression

    Mammalian

    Available Since

    Feb. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSprayerHE

    Plasmid

    #165150

    Purpose

    Sprayer plasmid for PROBER encoding BASU-GAL4 fusion (High Expression)

    Depositor

    Paul Khavari

    Article

    Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

    Insert

    BASU-GAL4
    

    Expression

    Mammalian

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. CD11b promoter in pGEM3zf(-)

    Plasmid

    #26168

    Depositor

    Daniel Tenen

    Article

    Dziennis et al Blood. 1995 Jan 15. 85(2):319-29.

    Insert

    CD11b promoter (ITGAM Human)
    

    Expression

    Bacterial

    Available Since

    Sept. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. human STIM1 YFP

    Plasmid

    #19754

    Depositor

    Anjana Rao

    Article

    Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

    Insert

    STIM1 (STIM1 Human)
    

    Use

    Retroviral

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    Oct. 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRRL-mU6-GFPGO2-IRES-mScarletI

    Plasmid

    #136896

    Purpose

    Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    GFPGO2-IRES-mScarletI
    

    Use

    Lentiviral

    Tags

    2xNLS on GFP, 3xNLS on mScarlet

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBait

    Plasmid

    #165146

    Purpose

    Bait plasmid for PROBER (empty backbone encoding replaceable ccdB cassette)

    Depositor

    Paul Khavari

    Article

    Mondal et al Nat Methods. 2022 Aug;19(8):959-968. doi

    Type

    Empty backbone

    Use

    Unspecified

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSimple-miR302/367

    Plasmid

    #98748

    Purpose

    Episomal reprogramming

    Depositor

    Sara Howden, James Thomson

    Article

    Howden et al Stem Cell Reports. 2015 Dec 8;5(6):1109-

    Insert

    miR302/367 (miRNA cluster) (MIR302A, MIR367 Human)
    

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge