We narrowed to 4,881 results for: phy

1. pUPD_OsU3 (GB1184)

   Plasmid

   #68209

   Purpose

   Provides the O. sativa U3 RNA polIII promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   OsU3 promoter
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPAtUbq10 (GB0223)

   Plasmid

   #68174

   Purpose

   Provides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtUbq10 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTYF-super-mfPENK-2.2k-Venus-WPRE

   Plasmid

   #156400

   Purpose

   Expresses Venus under PENK gene promoter

   Depositor

   Shuji Kaneko

   Article

   Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

   Inserts

   Venus
   Gal4p65
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJC1-Rap2A-3xFLAG

   Plasmid

   #87972

   Purpose

   Expression of Rap2A in mammalian cells

   Depositor

   David Sabatini

   Article

   Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPKm-243

   Plasmid

   #90506

   Purpose

   mOrange and mitochondrial-tagged sfGFP (pSIN-OFP-mitosfGFP)

   Depositor

   Todd Coleman

   Article

   Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

   Insert

   mOrange
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pPAct2noUTR (GB0192)

   Plasmid

   #68177

   Purpose

   Provides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtAct2 promoter (no UTR)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUHD 10.3-human MIZ1 delta POZ

   Plasmid

   #74160

   Purpose

   Tet inducible expression of MIZ1 delta POZ

   Depositor

   Martine Roussel

   Article

   Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

   Insert

   MIZ1 delta POZ (ZBTB17 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   delta POZ domain (first 109 aa)

   Promoter

   CMV2

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pBAD/HisB-PAiRFP2

   Plasmid

   #44271

   Depositor

   Vladislav Verkhusha

   Article

   Piatkevich et al Nat Commun. 2013 Jul 10;4:2153. doi: 10.

   Insert

   PAiRFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   K69R/R83K/G120D/A123T/M163L/Q168E/R220P/S243N/V24…

   Promoter

   pBAD

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-C1X-Nup88-mut

   Plasmid

   #87337

   Purpose

   To express human Nup88 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

   Depositor

   Lei Lu

   Article

   Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

   Insert

   NUP88 (NUP88 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   shRNA resistant changes

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTYF-super-mfSERT-1.9k-Venus-WPRE

    Plasmid

    #156394

    Purpose

    Expresses Venus under SERT gene promoter

    Depositor

    Shuji Kaneko

    Article

    Nagai et al Biochem Biophys Res Commun. 2019 Oct 22;

    Inserts

    Venus
    Gal4p65
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUB6_ATR

    Plasmid

    #84016

    Purpose

    ATR minigene to test splicing event

    Depositor

    Robert Bradley

    Article

    Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

    Insert

    ATR (ATR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UB6

    Available since

    Jan. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHcPro (GB0067)

    Plasmid

    #68196

    Purpose

    Provides the CDS of the Tobacco echt virus HcPro protein as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    TEV HcPro coding region
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pY71-tet.I.16

    Plasmid

    #133536

    Purpose

    sfGFP driven by a T7 promoter with the tetO sequence 19 bp downstream from the promoter start site

    Depositor

    Richard Murray

    Article

    McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

    Insert

    sfGFP
    

    Use

    Tags

    Strep tag and TEV site

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. Rae1-pDONR221

    Plasmid

    #124240

    Purpose

    Gateway entry clone of Drosophila Rae1 cDNA.

    Depositor

    Peter Vilmos

    Article

    Kristo et al Biochim Biophys Acta Mol Cell Res. 2017

    Insert

    Drosophila Rae1 (Rae1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. M26V DJ-1 pET15b

    Plasmid

    #60688

    Purpose

    Recombinant protein expression of Park7

    Depositor

    Mark Wilson

    Article

    Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

    Insert

    M26V DJ-1 (PARK7 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    M26V

    Promoter

    T7

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pTAtUbq3 (GB0219)

    Plasmid

    #68192

    Purpose

    Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    AtUbq3 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENTR-R4R3-stop

    Plasmid

    #113745

    Purpose

    Contains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    stop cassette
    

    Use

    CRISPR; Gateway entry vector

    Tags

    Expression

    Mutation

    Added 3 stop codons to the original sequence; see…

    Promoter

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS/U6/hTID1

    Plasmid

    #13745

    Depositor

    Karl Munger

    Article

    Edwards et al Oncogene. 2004 Nov 4. 23(52):8419-31.

    Insert

    hTid1 siRNA (DNAJA3 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Inverted repeat of 21 bp with 6 nuc. spacer.

    Promoter

    Available since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pDONR-IAA17_no stop

    Plasmid

    #83855

    Purpose

    Gateway donor vector for IAA17 with no stop codon

    Depositor

    Jennifer Nemhauser

    Article

    Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

    Insert

    IAA17 (AXR3 Mustard Weed)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    no stop codon

    Promoter

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. EGFP-hPMCA4b delta6

    Plasmid

    #47590

    Depositor

    Emanuel Strehler

    Article

    Padanyi et al Biochim Biophys Acta. 2009 Jun;1793(6):1

    Insert

    PMCA4bdelta6 (ATP2B4 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    6 C-terminal AA deleted (needed for PDZ binding)

    Promoter

    CMV

    Available since

    Aug. 26, 2013

    Availability

    Academic Institutions and Nonprofits only