We narrowed to 18,642 results for: RAN-1

1. JT113-pETDuet1-(R)-hREV3L

   Plasmid

   #64872

   Purpose

   bacterial expression of human REV3L

   Depositor

   Richard Wood

   Article

   Tomida et al Nucleic Acids Res. 2015 Jan;43(2):1000-1

   Insert

   REV3L (REV3L Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. p3xFLAG-MKL1

   Plasmid

   #11978

   Purpose

   For mammalian expression of human MKL1 with a 3x FLAG tag

   Depositor

   Ron Prywes

   Article

   Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

   Insert

   MKL1 (MRTFA Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N579S T716A

   Promoter

   CMV

   Available Since

   Aug. 10, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDV24_AAV2_DART-VADAR_iRFP720-sensor

   Plasmid

   #200411

   Purpose

   AAV vector encoding a DART-VADAR sensor for iRFP720 mRNA

   Depositor

   James Collins

   Article

   Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

   Insert

   DART VADAR sensor
   

   Use

   AAV and Synthetic Biology

   Tags

   MCP-ADAR2dd(E488Q)-NES, TagBFP, and mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJL1-sfGFP-DQNAT

   Plasmid

   #198210

   Purpose

   pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 30 amino acids containing an optimal DQNAT sequon followed by a 6x-His tag

   Depositor

   Michael Jewett

   Article

   Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

   Insert

   sfGFP_DQNAT
   

   Use

   Tags

   6x His Tag and DQNAT glycosylation tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pXD70LacZ6-LacI-Ptac(LacO)-LacZ

   Plasmid

   #191621

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible promoter-lacZ, engineered from pMAL-c2X

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   lacZ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST

   Plasmid

   #105448

   Purpose

   Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vector

   Depositor

   Hillel Adesnik

   Article

   Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

   Has Service

   AAV9

   Insert

   ChrimsonR-mRuby2-ST
   

   Use

   AAV

   Tags

   mRuby2 and soma targeting motif

   Expression

   Mutation

   K176R in Chrimson

   Promoter

   hSyn

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. ATF4 6: mCD36.GFP

   Plasmid

   #21853

   Depositor

   David Ron

   Article

   Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

   Insert

   mCD36 (Cd36 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Only the first 13 AA of CD36 are fused to GFP

   Promoter

   Available Since

   Sept. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GB4542

   Plasmid

   #215261

   Purpose

   Module for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   CBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GB4401

   Plasmid

   #215252

   Purpose

   Module for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   SF-PNOS:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MT1E

    Plasmid

    #105702

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 1e

    Depositor

    Artur Krężel

    Article

    Peris-Diaz et al J Proteome Res. 2021 Jan 1;20(1):776-785

    Insert

    Metallothionein 1e (MT1E Human)
    

    Use

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pXD70CT5.1-PxylR-CymR, Pveg(2CuO)-LacZ

    Plasmid

    #191628

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1 (tandem CymR binding sites)-LacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDT-sgRNA-XMAS-1x

    Plasmid

    #164413

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSCIP-mB2M

    Plasmid

    #154091

    Purpose

    Self-Cutting and Integrating CRISPR backbone targeting murine B2M

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    sgRNA and BAIT targeting murine B2M locus
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCDNA3-Myc3-Cul3 delta N41

    Plasmid

    #21590

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

    Insert

    cullin 3 (CUL3 Human)
    

    Use

    Tags

    myc3

    Expression

    Mammalian

    Mutation

    Residues 34-74 deleted from N terminus.

    Promoter

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

15. phGR-SB

    Plasmid

    #133409

    Purpose

    overexpression of a human glucocorticoid receptor in mammalian cells

    Depositor

    Andrew Cato

    Article

    Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

    Insert

    NR3C1 (NR3C1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available Since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTSara-N

    Plasmid

    #60721

    Purpose

    Expresses the N-terminal fragment of split T7 RNAP split a position 179. Insert is driven by PBAD. Contains a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    Residues 1-179 of T7 RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Only amino acids 1-179 of T7 RNAP

    Promoter

    PBAD

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP_D2A_tdTomato

    Plasmid

    #184045

    Purpose

    Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using a dual 2A peptide sequence (P2AT2A)

    Depositor

    Angela Pannier

    Article

    Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

    Inserts

    Enhanced Green Fluorescent Protein
    tdTomato
    Dual 2A Peptide Sequence
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA

    Plasmid

    #129708

    Purpose

    Express cre-dependent KASH-EGFP and scramble shRNA

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    KASH-EGFP
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1

    Plasmid

    #129706

    Purpose

    Express cre-dependent KASH-EGFP and Gmeb1 shRNA

    Depositor

    Yi Zhang

    Article

    Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

    Insert

    KASH-EGFP
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

    Plasmid

    #159803

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. myc-BioID-Rab7

    Plasmid

    #121049

    Purpose

    Expresses myc tagged BioID and human Rab7a in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

    Inserts

    BioID
    Rab7A
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    R118G (highly promiscuous form)

    Promoter

    CMV

    Available Since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUB-spHRed

    Plasmid

    #84734

    Purpose

    Expresses pHRed in plant cells-biosensor for pHRed targeted to chloroplasts stoma

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    pHRed
    

    Use

    Tags

    RbcS targeting sequence

    Expression

    Plant

    Mutation

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAT9650-BEAR-GFP-preedited

    Plasmid

    #162992

    Purpose

    BEAR control plasmid with split EGFP and intact 5' splice site

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    EGFP split with an intron between amino acids 95-96
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCSIIbsr-6013x

    Plasmid

    #108654

    Purpose

    An HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    Raichu-EV
    

    Use

    Lentiviral

    Tags

    XFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBEAST-J23101-sfGFP

    Plasmid

    #116917

    Purpose

    Strong constitutive expression of super-folder GFP for cell-free expression

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    sfGFP
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    J23101

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSLQ1371-sgGAL4

    Plasmid

    #121514

    Purpose

    sgGAL4 control. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Stanley Qi, Ovijit Chaudhuri

    Article

    Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

    Insert

    sgGAL4
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available Since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pACT2-μ3B

    Plasmid

    #198175

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP-3 μ3B
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GR A458T

    Plasmid

    #133410

    Purpose

    overexpression of a mutant human glucocorticoid receptor in mammalian cells

    Depositor

    Andrew Cato

    Article

    Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

    Insert

    NR3C1 (NR3C1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A458T

    Promoter

    Available Since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

    Plasmid

    #159799

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pMX-Flag-WIP

    Plasmid

    #11772

    Depositor

    Jack Strominger

    Article

    Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

    Insert

    WIP (WIPF1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

31. pXD70DA9-Pct5-DadhABC(A2)

    Plasmid

    #191632

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    Dopamine dehydroxylase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCAGGS-6020nes

    Plasmid

    #108656

    Purpose

    A JNK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-JNK-EV
    

    Use

    Tags

    XFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRSET a sfGCaMP6s

    Plasmid

    #100022

    Purpose

    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. cpEGFP replaced with a superfolder cpGFP variant.

    Depositor

    Wolf Frommer

    Article

    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

    Insert

    sfGCaMP6s
    

    Use

    Tags

    6x HIS tag and Xpress_EK tag

    Expression

    Bacterial

    Mutation

    cpEGFP replaced with superfolder cpGFP

    Promoter

    T7

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pUB-sSypHer (RbcS-SypHer)

    Plasmid

    #84732

    Purpose

    Expresses SypHer in plant cells-biosensor for pH targeted to chloroplasts stoma

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    sSypHer
    

    Use

    Tags

    RbcS targeting sequence

    Expression

    Plant

    Mutation

    Promoter

    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 1199 pAdTrack FKHR AAA

    Plasmid

    #9037

    Depositor

    William Sellers

    Article

    Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

    Insert

    FKHR AAA (FOXO1 Human)
    

    Use

    Adenoviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    T24A S256A S319A

    Promoter

    Available Since

    Sept. 28, 2005

    Availability

    Academic Institutions and Nonprofits only

36. pISceI Dusp6:memGFP

    Plasmid

    #32668

    Purpose

    Expresses membrane localized EGFP under the Dusp6 promoter

    Depositor

    Michael Tsang

    Article

    Wang et al Development. 2011 Jan;138(1):45-54. Epub

    Insert

    Dusp6 promoter (dusp6 Zebrafish)
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. Pet15b-Endosomal Escape Control

    Plasmid

    #186552

    Purpose

    Negative Control confirming the need for a CPP peptide in mediating internalization

    Depositor

    Jean Gariepy

    Article

    Mohammed et al J Control Release. 2012 Nov 28;164(1):58

    Insert

    6H-ETA-GFP
    

    Use

    Tags

    6xHis and EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Pet15b-Internalization and Endosomal Escape

    Plasmid

    #186551

    Purpose

    Internalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cells

    Depositor

    Jean Gariepy

    Article

    Mohammed et al J Control Release. 2012 Nov 28;164(1):58

    Insert

    10R-8His-ETA-EGFP
    

    Use

    Tags

    8xHis and EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. LEP-shREN

    Plasmid

    #105580

    Purpose

    retrovirally express control shRNA with puro resistance and GFP marker

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    control shRNA targeting luciferase
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTR

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p3XFLAG-MKL1-~100

    Plasmid

    #27176

    Depositor

    Ron Prywes

    Article

    Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

    Insert

    MKL1 (MRTFA Human)
    

    Use

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Deletion of 100 aa from ATG

    Promoter

    Available Since

    Dec. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

41. pCAGGS-6017nes

    Plasmid

    #108655

    Purpose

    A PKA biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-PKA-EV
    

    Use

    Tags

    XFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available Since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCAGGS-6015nes

    Plasmid

    #108657

    Purpose

    A S6 Kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    hyBRET-S6K-EV
    

    Use

    Tags

    XFP

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pQUAST-shibire (ts1)

    Plasmid

    #24362

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    Shibire (ts1) (shi Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCSIIbsr-6085x

    Plasmid

    #108653

    Purpose

    A tyrosine kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

    Depositor

    Michiyuki Matsuda

    Article

    Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

    Insert

    PicchuEV
    

    Use

    Lentiviral

    Tags

    XFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ

    Plasmid

    #191627

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZ

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    lacZ
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pACEC3GH (Apr)

    Plasmid

    #110085

    Purpose

    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pMJB1-RPS22B-Cl

    Plasmid

    #160431

    Purpose

    Expresses "cre-less" yeast RPS22B tagged with mCherry under the GAL1 promoter

    Depositor

    Gary Stormo

    Article

    Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

    Insert

    RPS22B (RPS22B Budding Yeast)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Removed introns, shuffled codons to change nucleo…

    Promoter

    GAL1 and S. cerevisiae GAL1

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pQCi-hTRAC-sgRNA

    Plasmid

    #154093

    Purpose

    pQCi backbone with sgRNA targeting human TCR-alpha common chain

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    sgRNA targeting human TRAC locus
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. SGL40C-H1.EFS.RFP657

    Plasmid

    #69148

    Purpose

    Advanced lentiviral Vector for sgRNA (H1 Promoter) delivery with RFP657 expression (EFS Promoter)

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    sgRNA
    EFS
    tagRFP657
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pBS U6 WASp RNAi

    Plasmid

    #11775

    Depositor

    Jack Strominger

    Article

    Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

    Insert

    WASp RNAi (WAS Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only