We narrowed to 10,838 results for: ENA

1. p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

   Plasmid

   #83485

   Purpose

   Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

   Insert

   Lbu_C2c2_R472A_H477A_R1048A_H1053A
   

   Use

   CRISPR

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   R472A, H477A, R1048A, H1053A

   Promoter

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAT005

   Plasmid

   #171632

   Purpose

   2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-B2M
   mNeon
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-a and U6

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSET-WHaloCaMP1a

   Plasmid

   #205304

   Purpose

   Expression of WHaloCaMP1a in E. coli

   Depositor

   Eric Schreiter

   Article

   Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

   Insert

   WHaloCaMP1a
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Sec61b-HA-mNG21-10

   Plasmid

   #141213

   Purpose

   Membrane Tethered Split Fluorescent Reporter for ER Retragrade

   Depositor

   Wayne Lencer

   Article

   Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

   Insert

   Sec61b-HA-mNG21-10
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. AA294

   Plasmid

   #215968

   Purpose

   Fragmid fragment: (Pol 2 promoter) U1 small nuclear RNA promoter

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   U1a_v1
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AA332

   Plasmid

   #216053

   Purpose

   Fragmid fragment: (C' terminus) 3x NLS sequences

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   SV40NLS_v1.10; SV40NLS_v1.11; SV40NLS_v1.12
   

   Use

   Fragment

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRetroX-Tight-MCS_PGK-GpNLuc

   Plasmid

   #70185

   Purpose

   Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

   Depositor

   Antonio Amelio

   Article

   Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. LOV-Turbo-V5_pRS415

   Plasmid

   #199667

   Purpose

   expresses LOV-Turbo in the yeast cytosol

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Tags

   V5

   Expression

   Yeast

   Mutation

   Promoter

   Gal1,10

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-EF1-IRES-NEO deleted ITR

   Plasmid

   #67906

   Purpose

   PiggyBac transposon encoding Neomycin resistance with deleted inverted terminal repeat

   Depositor

   Alex Kentsis

   Article

   Henssen et al Elife. 2015 Sep 25;4. pii: e10565. doi:

   Insert

   NeoR (neoR Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRDA_763

    Plasmid

    #201156

    Purpose

    EnAsCas12a with a deactivated DNAse domain (D908A), N' 5x tandem ALFA-tags and C' NLS for CRISPRa

    Depositor

    John Doench

    Article

    Griffith et al Cell Genom. 2023 Sep 1;3(9):100387. doi:

    Insert

    5x-ALFA tag-dEnAsCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    D908A

    Promoter

    EFS

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFDH

    Plasmid

    #131706

    Purpose

    Express constitutively an NAD-dependent formate dehydrogenase from Pseudomonas sp. 101 in E. coli

    Depositor

    Ron Milo

    Article

    Gleizer et al Cell. 2019 Nov 27;179(6):1255-1263.e12.

    Insert

    formate dehydrogenase
    

    Use

    Tags

    his-tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRetroX-Tight-MCS_PGK-OgNLuc

    Plasmid

    #70186

    Purpose

    Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

    Depositor

    Antonio Amelio

    Article

    Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCSF107mT-GATEWAY-3'-FLAG

    Plasmid

    #67619

    Purpose

    A pCS2 based vector to generate a 3'-Flag tag using GATEWAY cloning technology

    Depositor

    Todd Stukenberg

    Article

    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

    Type

    Empty backbone

    Use

    In vitro transcription or translation off of an s…

    Tags

    C-terminal Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SP6

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEY41

    Plasmid

    #191045

    Purpose

    dat-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    dat-1 (dat-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMGS54 (AID-P2A-Hygromycin)

    Plasmid

    #126583

    Purpose

    An AID-P2A-HygR cassette to tag the protein of interests with auxin-inducible degron at the c-terminal

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    AID-P2A-HygR
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSET-WHaloCaMP1b-EGFP

    Plasmid

    #205305

    Purpose

    Expression of WHaloCaMP1b-EGFP fusion in E. coli

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1b-EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEY11

    Plasmid

    #191017

    Purpose

    Fluorescent reporter driving panneuronal nuclear TagRFP-T expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    Ultrapanneuronal
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pREDIT_MCP-RecT

    Plasmid

    #164803

    Purpose

    mammalian-optimized expression of SSAP RecT fused to MCP (for MS2 aptamer), pLenti-EF1A-MCP-EXTEN-RecT-NLS

    Depositor

    Le Cong

    Article

    Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

    Insert

    RecT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLyGo-Ec-8

    Plasmid

    #163136

    Purpose

    pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P(rhaB)

    Available since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV hU6-sgRNA Nestin-dCas9-KRAB-T2a-GFP

    Plasmid

    #196988

    Purpose

    Derived from pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP, custom sgRNA cloning vector

    Depositor

    Elizabeth Kirby

    Article

    Dause et al Mol Neurobiol. 2025 Mar 25. doi: 10.1007

    Insert

    Nestin-dCas9-KRAB-T2a-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge