We narrowed to 8,806 results for: CAG

1. Luciferase shRNA-TRE

   Plasmid

   #225339

   Purpose

   shRNAmir backbone under tet-operator for RNA interference, with YFP

   Depositor

   Huda Zoghbi

   Article

   Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

   Insert

   Luciferase shRNA (LOC116160065 Mouse)
   

   Use

   AAV and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE (tetracycline-responsive element)

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd

   Plasmid

   #216172

   Purpose

   Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- Control- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TDP-43 mRuby K84ONBK

   Plasmid

   #216226

   Purpose

   TDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.

   Depositor

   Jennifer Lee

   Article

   Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

   Insert

   Transactive response DNA binding protein of 43 kDa (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Lysine 84 mutated to a TAG stop codon

   Promoter

   CMV Promoter

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. UCK2_Deletion_gRNA_1

   Plasmid

   #204672

   Purpose

   Dual gRNA plasmid for UCK2 deletion

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   uridine-cytidine kinase 2 (UCK2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. EGFP-ST-P2A-pSC-CAAX

   Plasmid

   #207637

   Purpose

   A plasmid encoding EGFP fused to SpyTag (ST) and photocaged SpyCatcher (pSC) localized to the cell membrane via CAAX tag.

   Depositor

   Cole DeForest

   Article

   Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

   Insert

   EGFP-SpyTag-P2A-photocaged SpyCatcher-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Amber stop codon at SC's critical lysine

   Promoter

   CMV

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgCTRL-puro

   Plasmid

   #199634

   Purpose

   sgRNA control guide targeting intergenic region

   Depositor

   William Hahn

   Article

   So et al JCI Insight. 2022 Oct 10;7(19):e158755.

   Insert

   N/A
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRANS-Pho2-1/2-2-Csy4

   Plasmid

   #161763

   Purpose

   pTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x Csy4-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)

   Depositor

   Shaun Curtin

   Article

   Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

   Insert

   Cas9 and gRNAs
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CmYLCV Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRANS-Pho2-1/2-2-tRNA

   Plasmid

   #161762

   Purpose

   pTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x tRNA-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)

   Depositor

   Shaun Curtin

   Article

   Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

   Insert

   Cas9 and gRNAs
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CmYLCV Promoter

   Available since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-guide-puro mRab12-1

   Plasmid

   #198475

   Purpose

   lentiviral stable expression of mRab12 gRNA 1

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mouse Rab12 (Rab12 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC1300_pUB10_pcoCASphi_E9t_V2_2x35Sp_HSP18t_ribozyme_AtPDS3_gRNA10

    Plasmid

    #197959

    Purpose

    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by 2x35Sp and flanked by ribozymes.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pcoCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S promoter and pUBQ10

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC1300_pUB10_pcoCASphi_E9t_V2_pUB10_E9t_ribozyme_AtPDS3_gRNA10

    Plasmid

    #197960

    Purpose

    T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by UBQ10 gene promoter and flanked by ribozymes.

    Depositor

    Steven Jacobsen

    Article

    Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

    Inserts

    pcoCasphi-2
    AtPDS3 gRNA10
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pUBQ10

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. mU6-sgCebpa_v1-hu6-sgCebpb_v1

    Plasmid

    #177251

    Purpose

    Expresses Cebpa_v1 (mU6), Cebpb_v1 (hU6) gRNAs and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

    Insert

    sgCebpa_v1/sgCebpb_v1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mU6/hU6

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. mU6-sgCebpa_v1-hu6-sgCebpd_v1

    Plasmid

    #177253

    Purpose

    Expresses Cebpa_v1 (mU6), Cebpd_v1 (hU6) gRNAs and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

    Insert

    sgCebpa_v1/sgCebpd_v1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mU6/hU6

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. mU6-sgCebpa_v2-hu6-sgCebpd_v2

    Plasmid

    #177254

    Purpose

    Expresses Cebpa_v2 (mU6), Cebpd_v2(hU6) gRNAs and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

    Insert

    sgCebpa_v2/sgCebpd_v2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mU6/hU6

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. hu6-sgCebpa_v1 (opti)

    Plasmid

    #177245

    Purpose

    Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpa gRNA

    Depositor

    Monte Winslow

    Article

    Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

    Insert

    sgCebpa_1st
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1-Tet-Puro-shMMERVK9c_1 (inducible)

    Plasmid

    #189957

    Purpose

    shRNA mediated knockdown

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    ERV_MMERVK9c
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO.1-Tet-Puro-shMMERVK9c_1

    Plasmid

    #185022

    Purpose

    shRNA mediated knockdown

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    ERV_MMERVK9c
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Tol2-LyzC-PBD-GFP-U6ac-ctrl guides

    Plasmid

    #168252

    Purpose

    "label active Rac; control for neutrophil-specific knockout"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    PBD GFP
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    LyzC

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides

    Plasmid

    #168253

    Purpose

    "label stable F-actin; control for neutrophil-specific knockout"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    Utrophin-GFP
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    LyzC

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ATP1A1_G3_MTOR_Nick_Intron-45_Dual_sgRNA

    Plasmid

    #178099

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 + MTOR nick intron-45 sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ATP1A1_G4_Q118R_MTOR_F2108L_Dual_pegRNA

    Plasmid

    #178102

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-F2108L pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R pegRNA + MTOR-F2108L pegRNA
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. ATP1A1_G4_Q118R_MTOR_I2017T_Dual_pegRNA

    Plasmid

    #178103

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-I2017T pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R pegRNA + MTOR-I2017T pegRNA
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ATP1A1_G8_MTOR_Nick_Exon-44_Dual_sgRNA

    Plasmid

    #178105

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G8 + MTOR nick exon-44 sgRNAs
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ATP1A1_G4_Q118R_MTOR_E2419K_Dual_pegRNA

    Plasmid

    #173209

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-E2419K pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R pegRNA + MTOR E2419K pegRNA
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ATP1A1_G3_MTOR_Nick_Exon-53_Dual_sgRNA

    Plasmid

    #173211

    Purpose

    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick exon-53 sgRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G3 sgRNA + MTOR Nick exon-53 sgRNA
    

    Use

    CRISPR; Prime editing (pe3)

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ATP1A1_G4_Q118R_RUNX1_+4_T_to_G_Dual_pegRNA

    Plasmid

    #173212

    Purpose

    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and RUNX1 +4 T to G pegRNAs from two independent U6 promoters.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R pegRNA + RUNX1 +4 T to G pegRNA
    

    Use

    CRISPR; Prime editing

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tandem U6 promoters

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSC51

    Plasmid

    #104825

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoter

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Insert

    Glyma.12g075700, Glyma.11g145900
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PLKO.1-UPF3B-3'UTR

    Plasmid

    #136044

    Purpose

    UPF3B shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (CAGGGCAAAGAATAGAGAGAA)

    Depositor

    Anthony Leung

    Article

    Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

    Insert

    UPF3B (UPF3B Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPN273

    Plasmid

    #91644

    Purpose

    Express sgRNA targeting human MMP16

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    MMP16 (MMP16 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. NSL1 C6.4 gRNA

    Plasmid

    #90805

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NSL1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    NSL1 (Guide Designation C6.4)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge