We narrowed to 8,884 results for: tre promoter
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QUAS-0-T7-TetO-GFP_ColE1
Plasmid#171662PurposeQUAS directly upstream of a T7 promoter with a TetO operator. Expresses GFP in BL21(DE3) E. coli. Contains the ColE1 origin of replication.DepositorInsertQUAS-T7-TetO-GFP ssrA-T7 Stop
UseTagsssrA degradation tag (DAS+4)ExpressionBacterialMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136457
Plasmid#149537PurposeBacterial expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTags6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEVExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046858
Plasmid#145658PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046730
Plasmid#145659PurposeBacterial Expression plasmid for SARS-CoV-2 E (envelope)DepositorInsertSARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046823
Plasmid#145634PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;MycExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046816
Plasmid#145622PurposeBacterial Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferaseDepositorInsertSARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;FLAG_2ExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
MtMscL delta49
Plasmid#118342PurposeM. tuberculosis MscL (delta49) in pET19bDepositorInsertMscL (mscL Mycobacterium tuberculosis)
UseTags6x His Tag and Thrombin Cleavage siteExpressionBacterialMutationChanged Glutamic Acid (Glu) 102 to Stop CodonPromoterT7 PromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;cldn5bCE1-basEgfp
Plasmid#90136Purposecontains cldn5b enhancer sequence and a basal promoter driving expression of eGFPDepositorInsertcldn5bE1basEgfp (cldn5b Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;yrkP1Egfp
Plasmid#90168Purposecontains yrk specific gene promoter driving expression of eGFPDepositorInsertyrkP1Egfp (yrk Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;snx5P1Egfp
Plasmid#90166Purposecontains snx5 specific gene promoter driving expression of eGFPDepositorInsertsnx5P1Egfp (snx5 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;sheCE1-P1Egfp
Plasmid#90150Purposecontains She enhancer sequence, and She specific gene promoter driving expression of eGFPDepositorInsertsheE1P1Egfp (she Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;tmem88aCE1-basEgfp
Plasmid#90135Purposecontains tmem88a enhancer sequence and a basal promoter driving expression of eGFPDepositorInserttmem88aE1basEgfp (tmem88a Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Laccase2 Sense
Plasmid#69883PurposeExpresses the Drosophila laccase2 exon 2 circular RNADepositorInsertLaccase2 (stw Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pET11+PCNA3-CYP119(D77R/T214V)
Plasmid#66138PurposeExpresses the D77R/T214V mutant of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
UseTagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationD77R, T214VPromoterT7 promoterAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL3-PIM2mut2
Plasmid#51850Purposepromoter region of PIM2 lacking the forkhead binding site #1DepositorInsertPIM2 (PIM2 Human)
UseLuciferase; Reporter vectorTagsExpressionMutationPromoterAvailable sinceJuly 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEG634
Plasmid#40121DepositorInsertMex-5 (aa244-468) (mex-5 Nematode)
UseGateway destination vectorTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationcontains aa244-468Promoter4.4 kb mex-5 promoter fragmentAvailable sinceSept. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pFSW Syt1-C2B R398,399Q IRES GFP
Plasmid#133819PurposeEncodes the C2B domain of synaptotagmin 1 with mutations R398,399Q for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationR398,399QPromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pFSW Syt1-C2B K326,327E IRES GFP
Plasmid#133820PurposeEncodes the C2B domain of synaptotagmin 1 with mutations K326,327E for viral expressionDepositorInsertSyt1 (Syt1 Rat)
UseLentiviralTagsIRES-GFPExpressionMutationK326,327EPromoterhSyn (human synapsin I promoter)Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-SpCas9
Plasmid#104588PurposeAAV vector expressing Myc-tagged SpCas9 under the EFS promoterDepositorInsertStreptococcus pyogenes Cas9
UseAAV and CRISPRTagsMycExpressionMammalianMutationPromoterEFSAvailable sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX Nup62Fv2GFP
Plasmid#139683PurposeNup62 dimerization construct fused to 2 copies of GFP under an CMV promoterDepositorInsertNup62 (NUP62 Human)
UseLentiviralTags2 copies of GFP and Dimerization domain FKBPExpressionMammalianMutationPromoterCMVAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-zeo_3xHA-TurboID-NLS
Plasmid#200022PurposeFor lentiviral transduction of 3xHA-TurboID-NLS under control of a doxycyline-inducible promoterDepositorInsert3xHA-TurboID-NLS
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GsAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
slc1a3b:myrGFP-P2A-H2AmCherry
Plasmid#170208PurposeDrives myrGFP-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGFP labels the membrane and mCherry labels the nucleusDepositorInsertslc1a3b (slc1a3b Zebrafish)
UseZebrafishTagsmyrGFP-P2A-H2AmCherryExpressionMutationPromoterslc1a3bAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
hRad51(A89E)–Cas9(D10A)
Plasmid#125563PurposeExpresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoterDepositorInsertRad51(A89E)–Cas9(D10A) (RAD51 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSH424-ssr
Plasmid#198026PurposeExpresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1EDepositorInsertT0 terminator - Sm resistance cassette
UseTagsExpressionBacterialMutationPromoterSmR promoterAvailable sinceOct. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b-PelB-6H-Ag43-160N-sfGFP
Plasmid#172886PurposeDisplay sfGFP protein on E. coli cell surfaceDepositorInsertsUseTagsHHHHHH and TEV recognition site (ENLYFQG)ExpressionBacterialMutationDeleted 1-160 (N terminal)PromoterT7 PromoterAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLC-217
Plasmid#73163PurposeTemplate plasmid which encodes a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) to be used as a negative selection marker.DepositorInsertPrhaB-relE (relE E. coli (bacteria))
UseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 apmA
Plasmid#112881PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertapmA (apmA Staphylococcus aureus)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 rmtB
Plasmid#112880PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertrmtB (RmtB Escherichia coli)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP4 ermC
Plasmid#112884PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorInsertermC (ermM Staphylococcus epidermis)
UseTags6xHisExpressionBacterialMutationPromoterPlacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 cat
Plasmid#112888PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertcat (Brevibacillus brevis) (cat Brevibacillus brevis)
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFsynW SYT1 reporter
Plasmid#197275PurposeEncodes for viral expression of wild type Synaptotagmin 1 (SYT1) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).DepositorInsertSYT1 and HaloTag (Syt1 Rat)
UseLentiviralTagsFLAGExpressionMutationN/APromoterhSyn (human synapsin I promoter)Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAIP Nup62Fv2GFP
Plasmid#139684PurposeNup62 dimerization construct fused to 2 copies of GFP under an SFFV promoterDepositorInsertNup62 (NUP62 Human)
UseLentiviralTags2 copies of GFP and Dimerization domain FKBPExpressionMammalianMutationPromoterSFFVAvailable sinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only
