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Munc18cF119E-pDEST27

Plasmid

#85625

Purpose

GST tagged Munc18c with a F119E mutation

Depositor

David James

Article

Latham et al Traffic. 2006 Oct;7(10):1408-19. Epub 20

Insert

Munc18c (Stxbp3 Mouse)

Tags

GST

Expression

Mammalian

Mutation

F119E

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2381

Plasmid

#36790

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

ABL1-TALEN_Right (Abl1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pMaCTag-25

Plasmid

#120004

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with YFP-LID

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

YFP-LID

Use

Pcr template

Tags

YFP-LID

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-19

Plasmid

#124787

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mCherry-mNeonGreen

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mCherry-mNeonGreen

Use

Pcr template

Tags

mCherry-mNeonGreen

Promoter

None

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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mOC-STAMP_pcDNA3.1 D/V5-His-TOPO

Plasmid

#73448

Purpose

Express mouse OC-STAMP in mammalian cells

Depositor

Paul Odgren

Article

Yang et al J Cell Physiol. 2008 May;215(2):497-505.

Insert

Ocstamp (Ocstamp Mouse)

Tags

V5-6xHis

Expression

Mammalian

Mutation

N162D

Available Since

April 5, 2016

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1R

Plasmid

#68230

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPromE8 (GB0914)

Plasmid

#68175

Purpose

Provides the S. Lycopersicum fruit promoter E8 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlE8 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDD401

Plasmid

#91828

Purpose

Pmyo-2::GFP FP-slot donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Pmyo-2::GFP

Use

CRISPR

Expression

Worm

Mutation

Nucleotide 489 of the myo-2 promoter was changed …

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pT35S (GB0036)

Plasmid

#68187

Purpose

Provides the CaMV 35S terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

CaMV 35S terminator

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMaCTag-P03

Plasmid

#120014

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with moxCerulean3

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

moxCerulean3

Use

Pcr template

Tags

moxCerulean3

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-kanMX6-P3nmt1-mEGFP

Plasmid

#105137

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

Insert

kanMX6-P3nmt1-mEGFP

Tags

3nmt1-mEGFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

3nmt1

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAL942

Plasmid

#119721

Purpose

precursor slow-folding rbsB with the mutations Alanine 248 Threonine, Alanine 188 Cysteine and Glutamic acid 246 Cysteine

Depositor

Linda Randall

Article

Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

Insert

precusor slow-folding ribose import binding protein, rbsB, mutations A248T, A118C and E246C

Tags

none

Expression

Bacterial

Mutation

Alanine 248 to Threonine, Alanine 188 to Cysteine…

Promoter

T7

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pFbx15(-640/-527)-TK-Luc

Plasmid

#13413

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pFbx15(-701/+114)-Luc

Plasmid

#13409

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pAGH51

Plasmid

#107255

Purpose

HU-FRB construct (anchor) expressed in trans from the slr0168 neutral site of Synechocystis PCC6803. Used to anchor away any FKBP tagged target in presence of rapamycin.

Depositor

Erin O'Shea

Article

Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

Insert

HU-FRB

Use

Synthetic Biology

Tags

FRB

Promoter

native HU promoter

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha2R

Plasmid

#68231

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b+PCNA2 L171C

Plasmid

#66177

Purpose

Expresses the L171C mutant of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Mutation

L171C

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b+PCNA1 G108C

Plasmid

#66140

Purpose

Expresses the G108C mutant of Sulfolobus solfataricus PCNA1

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 1

Tags

His6 tag

Expression

Bacterial

Mutation

G108C

Promoter

T7 promoter

Available Since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-bSV-831-1792

Plasmid

#40961

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-830

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
EGFP-bSV-1010-1792

Plasmid

#40959

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-1009

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only
TAL2266

Plasmid

#36703

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FH-TALEN_Left (FH Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2267

Plasmid

#36704

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FH-TALEN_Right (FH Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2405

Plasmid

#36808

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CDK4-TALEN_Right (Cdk4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2404

Plasmid

#36807

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CDK4-TALEN_Left (Cdk4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
Human NCKX3 (pcDNA3.1+)

Plasmid

#75205

Purpose

Mammalian expression of SLC24A3

Depositor

Jonathan Lytton

Article

Kraev et al J Biol Chem. 2001 Jun 22;276(25):23161-7

Insert

SLC24A3 (SLC24A3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2202

Plasmid

#36665

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CDC73-TALEN_Left (Cdc73 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2203

Plasmid

#36666

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

CDC73-TALEN_Right (Cdc73 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2252

Plasmid

#36695

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

EWSR1-TALEN_Left (Ewsr1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2253

Plasmid

#36696

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

EWSR1-TALEN_Right (Ewsr1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Ywhag-3'UTR

Plasmid

#61778

Purpose

Luciferase reporter containing the 3'UTR of mouse Ywhag

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Ywhag 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
psiCHECK-2-Xbp1-3'UTR

Plasmid

#61779

Purpose

Luciferase reporter containing the 3'UTR of mouse Xbp1

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

mouse Xbp1 3'UTR

Use

Luciferase

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only
pP2A11 (GB0082)

Plasmid

#68168

Purpose

Provides the S. Lycopersicum fruit promoter 2A11 as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Sl2A11 promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b+PCNA3 WT

Plasmid

#66179

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pET15b+PCNA2 WT

Plasmid

#66175

Purpose

Expresses the wild type of Sulfolobus solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

Insert

DNA polymerase sliding clamp 2

Tags

His6 tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 30, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2204

Plasmid

#36667

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

EXT2-TALEN_Left (Ext2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2205

Plasmid

#36668

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

EXT2-TALEN_Right (Ext2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pFbx15(-1024/+114)-Luc

Plasmid

#13410

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
TAL2415

Plasmid

#36816

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

EXT1-TALEN_Right (Ext1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available Since

July 13, 2012

Availability

Academic Institutions and Nonprofits only
M19 pCS-twhh-dsl-1myc

Plasmid

#17156

Depositor

Randall Moon

Article

Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Insert

dorsalin-1 (GDF2 Chicken)

Tags

myc-tag and twhh promoter (D rerio)

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pENTR-fishRab8B[Q67L]

Plasmid

#24908

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

Rab8B (rab8b Zebrafish)

Use

Gateway entry vector

Mutation

Changed Glutamine 67 to Leucine

Available Since

June 28, 2010

Availability

Academic Institutions and Nonprofits only
pFbx15(-544/-527)-TK-Luc

Plasmid

#13414

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pFbx15(-638/+114)-Luc

Plasmid

#13408

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Upstream region of mouse Fbx15 gene

Use

Luciferase

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
FSW FLAG

Plasmid

#246725

Purpose

Neuron-specific expression of insert with a FLAG tag on its N-terminus after cloning

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
FSW mKeima

Plasmid

#246726

Purpose

Neuron-specific expression of insert with a mKeima tag on its N-terminus after cloning

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Tags

mKeima

Expression

Mammalian

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-MitoRlucN-T2A-RlucCER-IRES-mCherry

Plasmid

#249563

Purpose

For detection of ER-mitochondrial contact

Depositor

Ginam Cho, Jeffrey Golden, Youngshin Lim

Article

Chen et al J Vis Exp. 2024 Oct 11;(212):10.3791/668

Insert

MitoRlucN-T2A-RlucCER

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Promoter

Chicken beta-actin promoter with CMV enhancer

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_DualsgRNA_CTRL_Puro_T2A_BFP2

Plasmid

#236730

Purpose

Dual sgRNA targeting CTRL expressing BFP with Puromycin selection

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

Negative CTRL

Use

Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_DualsgRNA_CTRL_ER-dAPEX-BFP2

Plasmid

#236733

Purpose

Dual sgRNA targeting CTRL expressing dAPEX-BFP targeting to ER

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

Negative CTRL

Use

CRISPR and Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pX459-Fzd1/2 gRNA

Plasmid

#246562

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd1/2 gRNA (conserved region)

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd1/2 gRNA

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW-EGFP-Blast

Plasmid

#246561

Purpose

Lentiviral vector for inducible expression of EGFP (all-in-one Tet-On system)

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

GFP

Use

Lentiviral

Mutation

F64L, S65T (EGFP)

Promoter

tight TRE

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABE-Puro-BirA

Plasmid

#246557

Purpose

Retroviral expression vector for secreted E. coli BirA, C-terminal FLAG tag

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

BirA

Use

Retroviral

Tags

FLAG

Promoter

MMLV LTR

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 7701 - 7750 of 17163 results

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We narrowed to 17,163 results for: Emb;