We narrowed to 5,036 results for: U6...

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pORANGE Cacnb3-GFP KI

Plasmid

#139662

Purpose

Endogenous tagging of CaVβ3: C-terminal (amino acid position: W479)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-TUBA1B

Plasmid

#207763

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSL3617 (pCRISPR(BbsI)_Pse)

Plasmid

#200902

Purpose

Expresses Pseudoalteromonas CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

PseCRISPR (entry)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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TNPO1-shRNA

Plasmid

#86082

Purpose

A lentiviral construct for building stable cell lines expressing Transportin-1 shRNA via puromycin selection.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Transportin-1 (TNPO1 Human)

Expression

Mammalian

Promoter

U6

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRDA_526

Plasmid

#184859

Purpose

Lentiviral vector that enables constitutive sgRNA expression. Also encodes Thy1.1 as a marker of transduction.

Depositor

Arlene Sharpe

Article

Milling et al J Exp Med. 2024 Apr 1;221(4):e20230699.

Insert

sgRNA cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE

Plasmid

#176273

Purpose

Expression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI sgRNA (sgTet) and BFP

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pWN_U6-B2M-miniGag-Cas9

Plasmid

#228958

Purpose

miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag-Cas9

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pORANGE LOX Cloning template vector

Plasmid

#139651

Purpose

Cloning template for ORANGE-CAKE conditional knock-in constructs

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pdCas9-Tet1-CD

Plasmid

#83340

Purpose

To introduce dCas9-fused Tet1-CD and sgRNA2.0 system

Depositor

Ronggui Hu

Article

Xu et al Cell Discov. 2016 May 3;2:16009. doi: 10

Insert

dCas9-Tet1-CD and sgRNA scaffold with 2xMS2 binding sites

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

U6, CBH

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPUR-mU6-sgRNA-2XPP7

Plasmid

#194504

Purpose

Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

Depositor

Chunqing Song

Article

Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSpCas9-2A-Puro-TBK1-gRNA (PX459)

Plasmid

#221550

Purpose

Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al EMBO J. 2024 Sep;43(18):3948-3967. doi:

Insert

gRNA targeting human TBK1 (TBK1 Human)

Expression

Mammalian

Promoter

u6

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-eCas9-sgNT

Plasmid

#140238

Purpose

Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

non-targeting sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-pegRNA-RNF2_+5GtoT

Plasmid

#135957

Purpose

S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_5GtoT pegRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAcCas12nHS_RV6_empty

Plasmid

#203811

Purpose

Plasmid containing a CMV-driven NLS-AcCas12n-NLS (human-codonoptimized) cassette and a U6-driven sgRNA_V6 cassette (containing BsaI sites for spacer insertion). Carbenicillin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

AcCas12nHS_RV6

Use

CRISPR

Promoter

CMV

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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gRNA-HEK3-Puro

Plasmid

#136282

Purpose

Expresses a guide RNA targeting HEK3 site

Depositor

Zhaohui Ye

Article

McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:

Insert

sgRNA-HEK3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-guide-puro mSptlc2 - 2

Plasmid

#198494

Purpose

lentiviral stable expression of mSptlc2 gRNA 2

Depositor

Suzanne Pfeffer

Article

Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

Insert

mSptlc2 (Sptlc2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pU6-sp-sgRNA-RNF2_+41nick

Plasmid

#135958

Purpose

S. pyogenes sgRNA for +41 nick in RNF2

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_+41 nicking sgRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-CETN2

Plasmid

#227291

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of CETN2 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of CETN2 (CETN2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro

Plasmid

#211687

Purpose

U6-sgRNA-EFS-Cas9-P2A-Puro

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

SpyCas9 and guide RNA recipient vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pDGE334

Plasmid

#153243

Purpose

M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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