162,309 results

1. lentiCRISPR v2-sgSLC7A11/xCT-2

   Plasmid

   #161819

   Purpose

   knock out SLC7A11/xCT in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2020 Apr;22(4):476-486. d

   Insert

   solute carrier family 7 member 11 (SLC7A11 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CaMKII-GFP

   Plasmid

   #64545

   Purpose

   AAV-mediated expression of GFP under the CaMKII promoter.

   Depositor

   Edward Boyden

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC-hrDNA-12.0

   Plasmid

   #206320

   Purpose

   A plasmid containing a 12.0 kb EcoRI fragment that corresponds to sequences between 30.5 and 42.5 kb of the human rDNA repeat (GenBank: U13369)

   Depositor

   Brian McStay

   Article

   van Sluis et al Methods Mol Biol. 2016;1455:3-14. doi: 1

   Insert

   Intergenic spacer of human rDNA repeat
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMXS-GshF-blasticidin

   Plasmid

   #210313

   Purpose

   Expresses humanized Streptococcus thermophilus GshF in mammalian cells

   Depositor

   Kivanc Birsoy

   Article

   Wang et al Nature. 2021 Nov;599(7883):136-140. doi:

   Insert

   Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
   

   Use

   Retroviral

   Tags

   FLAG peptide

   Expression

   Mutation

   Codon optimized for expression in human cells

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. NylCp2-TS_pET28a

   Plasmid

   #215421

   Purpose

   Expression construct for NylCp2-TS gene, codon optimized for expression in E. coli

   Depositor

   Gregg Beckham

   Article

   Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

   Insert

   NylCp2-TS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D122G/H130Y/D36A/E263Q

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSLIK CXADR-V5 hygro

   Plasmid

   #220362

   Purpose

   Lentiviral expression vector for an inducible V5-tagged CXADR construct

   Depositor

   Kevin Janes

   Insert

   CXADR (CXADR Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1(+)-Cyto-CaNARi

   Plasmid

   #64849

   Purpose

   Cytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNARi
   

   Use

   Tags

   Cerulean, Nuclear export signal (NES), and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. GFP-MAPPER

   Plasmid

   #117721

   Purpose

   MAPPER Selectively Labels ER-PM Junctions

   Depositor

   Jen Liou

   Article

   Chang et al Cell Rep. 2013 Nov 14;5(3):813-25. doi:

   Insert

   MAPPER
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. 3xFLAG-CUL3-pCMV7.1

   Plasmid

   #155021

   Purpose

   N-terminal 3xFLAG-tagged CUL3 in a pCMV7.1 vector

   Depositor

   Andre Catic

   Article

   Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

   Insert

   Cullin 3 (CUL3 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Promoter

   pCMV7.1

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-GFP-TurboID-NES_pLX304

    Plasmid

    #202019

    Purpose

    expresses GFP-TurboID in the mammalian cytosol, lentiviral vector

    Depositor

    Alice Ting

    Article

    Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

    Insert

    Flag-GFP-TurboID-NES
    

    Use

    Lentiviral

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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