164,881 results

1. TRIM33-GFP

   Plasmid

   #65399

   Purpose

   GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

   Depositor

   Kyle Miller

   Article

   Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

   Insert

   TRIM33 (TRIM33 Human)
   

   Use

   Tags

   EmGFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   May 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET29b-AK1-His

   Plasmid

   #124135

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Adenylate kinase (AK1 Chicken)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. deltaNp63alpha-FLAG

   Plasmid

   #26979

   Depositor

   David Sidransky

   Article

   Chatterjee et al Cell Cycle. 2008 May 1. 7(9):1231-7.

   Insert

   ΔNP63alpha (TP63 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   dominant negative isoform of p63; aa 1–587

   Promoter

   Available Since

   Dec. 15, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. SepRS(2)/pSertRNA(B4)/EF-Sep

   Plasmid

   #173897

   Purpose

   The plasmid contains an orthogonal aminoacyl-tRNA synthetase/tRNACUA pair, which directs the efficient incorporation of phosphoserine (pSer) into recombinant proteins in Escherichia coli.

   Depositor

   Jason W Chin

   Article

   Rogerson et al Nat Chem Biol. 2015 Jul;11(7):496-503. d

   Inserts

   SepRS(2)
   pSertRNA
   EF-Sep
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV p16 INK4A

   Plasmid

   #10916

   Depositor

   Bob Weinberg

   Article

   Medema et al Proc Natl Acad Sci U S A. 1995 Jul 3. 92

   Insert

   p16 INK4 (CDKN2A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA

   Plasmid

   #113691

   Purpose

   U6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Insert

   SaCas9
   

   Use

   AAV, CRISPR, and Cre/Lox

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Cytomegalo Virus(CMV)

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1+ hnRNPK myc HIS

   Plasmid

   #71662

   Purpose

   Mammalian expression of hnRNPK

   Depositor

   Gideon Dreyfuss

   Insert

   hnRNPK (HNRNPK Human)
   

   Use

   Tags

   HIS and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNO286-3

   Plasmid

   #107746

   Purpose

   Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)

   Depositor

   Jeffrey Tabor

   Article

   Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb

   Inserts

   CcaS
   Ho1
   PcyA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Deleted AA 223 and 512

   Promoter

   J23106 and J23108

   Available Since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. LRRK2-WT

   Plasmid

   #17609

   Depositor

   Ted Dawson

   Article

   West et al Proc Natl Acad Sci U S A. 2005 Nov 15;10

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKD3_mCherry

    Plasmid

    #187385

    Purpose

    To chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRT

    Depositor

    Roi Avraham

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYC01

    Plasmid

    #224586

    Purpose

    IPTG-inducible phage φX174 lysis gene E

    Depositor

    Ophelia Venturelli

    Article

    Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

    Insert

    phage φX174 lysis gene E (phiX174p08 )
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PA1lacO-1

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEmerald-Fascin-C-10

    Plasmid

    #54094

    Purpose

    Localization: Actin Bundling Protein, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Fascin (FSCN1 Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pAAV-TRE-mNeonGreen

    Plasmid

    #99131

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein mNeonGreen

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mNeonGreen
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-CaMKIIa-mCherry

    Plasmid

    #114469

    Purpose

    mCherry Control

    Depositor

    Karl Deisseroth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFA6a-link-ymNeongreen-URA3

    Plasmid

    #168059

    Purpose

    Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymNeonGreen
    

    Use

    Tags

    ymNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPR-v2-HygR-EGFP

    Plasmid

    #167188

    Purpose

    Lentiviral expression of sgRNA with hygromycin resistance gene and EGFP

    Depositor

    Yin Shen

    Article

    Ren et al Sci Adv. 2021 Sep 17;7(38):eabi4360. doi

    Insert

    Hygromycin B phosphotransferase and EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3 MDM2 WT

    Plasmid

    #16233

    Depositor

    Mien-Chie Hung

    Article

    Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. COC053

    Plasmid

    #209131

    Purpose

    Enzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.

    Depositor

    Christopher A. O'Callaghan

    Article

    O'callaghan et al Anal Biochem. 1999 Jan 1;266(1):9-15. do

    Insert

    Enzyme BirA (birA E. coli)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCFJ601 - Peft-3 Mos1 transposase

    Plasmid

    #34874

    Purpose

    Mos1 transposase expression vector for use in creating targeted transgene insertions and targeted deletions in C. elegans

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    Mos1 transpoase
    

    Use

    Worm targeting

    Tags

    Expression

    Mutation

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. Spectrin-M-cpstFRET

    Plasmid

    #61109

    Purpose

    Expression of Spectrin-cpstFRET to measure mechanical stress and report multidimensional stress fields

    Depositor

    Fred Sachs

    Article

    Meng et al J Cell Sci. 2012 Feb 1;125(Pt 3):743-50.

    Insert

    spectrin-cpstFRET
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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