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pSMART-sgRNA (Sp)

Plasmid

#80427

Purpose

U6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.

Depositor

Sara Howden, Melissa Little

Publication

Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

Type

Empty backbone

Expression

Mammalian

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pAAV-hsyn-flex-dsRed-shvgat

Plasmid

#67845

Purpose

Expresses shRNA to the vesicular GABA transporter VGAT. Cre dependent expression by flex switch

Depositor

William Wisden

Publication

Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

Insert

AAV-hsyn-flexed-dsRed-miR30-vgat2.shRNA

Use

AAV, Cre/Lox, Mouse Targeting, and RNAi

Expression

Mammalian

Promoter

h-synapsin

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSIN4-EF1a-GATA1-IRES-Puro

Plasmid

#61062

Purpose

lentiviral vector for GATA1

Depositor

Igor Slukvin

Publication

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

GATA1 (GATA1 Human)

Use

Lentiviral

Tags

Flag

Promoter

EF1alpha

Available since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHL-EF1a-SphcCas9-iP-A

Plasmid

#60599

Purpose

Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

Depositor

Akitsu Hotta

Publication

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Insert

CRISPR Cas9

Use

CRISPR

Expression

Mammalian

Mutation

Codon usage optimized for human usage

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pICE-FLAG-NAT10-siR-WT

Plasmid

#59365

Purpose

Plasmid for constitutive or doxycycline-inducible expression of wild-type human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Publication

Larrieu et al Science. 2014 May 2;344(6183):527-32. do

Insert

human NAT10 (NAT10 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Mutations to render cDNA resistant to a siRNA

Promoter

CMV-tet

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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mKate1.31-pBAD

Plasmid

#54827

Purpose

Localization: Protein Expression Vector , Excitation: 588, Emission: 635. Also known as mKate2.

Depositor

Michael Davidson

Publication

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Type

Empty backbone

Tags

6xHis and mKate1.31 (aka mKate2)

Expression

Bacterial

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pKSI-1

Plasmid

#51725

Purpose

cloning backbone

Depositor

Sheng Yang

Publication

Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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GFP-Rab5DN(S34N)

Plasmid

#35141

Depositor

Sergio Grinstein

Publication

Bohdanowicz et al Mol Biol Cell. 2012 Jan;23(1):176-87. Ep

Insert

Rab5a (RAB5A Human)

Tags

EGFP

Expression

Mammalian

Mutation

Dominant Negative- S34N

Promoter

CMV

Available since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pTorPE-R-GECO1

Plasmid

#32465

Depositor

Robert Campbell

Publication

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

R-GECO1.0

Use

Lab constructed

Tags

6x His and Modified TorA MNNNDLFQASRRRFLAQLG[G to…

Expression

Bacterial

Mutation

Substitutions relative to the mApple-derived anal…

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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GST-YAP2

Plasmid

#24637

Depositor

Kunliang Guan

Insert

Yap2 (YAP1 Human)

Tags

GST

Expression

Bacterial

Available since

Sept. 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDONR223-FGFR2

Plasmid

#23904

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FGFR2 (FGFR2 Human)

Use

Gateway Cloning

Mutation

A52D, T525A (compared to GenBank reference sequen…

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-TYK2

Plasmid

#23908

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TYK2 (TYK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBS-hBAF60c

Plasmid

#21036

Depositor

Jerry Crabtree

Publication

Wang et al Genes Dev. 1996 Sep 1. 10(17):2117-30.

Insert

BAF60c (SMARCD3 Human)

Use

Phagemid

Available since

July 24, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
psiCHECK2-let-7 8x

Plasmid

#20931

Depositor

Yukihide Tomari

Publication

Iwasaki et al Mol Cell. 2009 Apr 10;34(1):58-67. doi:

Insert

8x let-7 target sites

Use

Luciferase

Expression

Insect and Mammalian

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pRK5-HA GST RagA 21L

Plasmid

#19299

Depositor

David Sabatini

Publication

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding A (RRAGA Human)

Tags

GST and HA

Expression

Mammalian

Mutation

T21L

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles
pcDNA3 myc card9

Plasmid

#16253

Depositor

William Kaelin

Publication

Yang et al Mol Cell. 2007 Oct 12. 28(1):15-27.

Insert

card9 (CARD9 Human)

Tags

myc

Expression

Mammalian

Available since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

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G-RCAS (A)

Plasmid

#15182

Depositor

Connie Cepko

Publication

Harpavat et al BMC Dev Biol. 2006 Jan 22;6:2. doi: 10.1

Insert

Gateway cassette

Use

RNAi and Retroviral; Avian expression

Available since

June 15, 2007

Availability

Academic Institutions and Nonprofits only

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pET15b-Casp8 delta DED

Plasmid

#11827

Depositor

Guy Salvesen

Publication

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 8 minus Death Effecter Domain (CASP8 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Minus Death Defecter Domain (aa 1-216)

Available since

May 12, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
Rc/CMV cyclin D1

Plasmid

#8962

Depositor

Bob Weinberg

Publication

Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

Insert

cyclin D1 (CCND1 Human)

Expression

Mammalian

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
Arrb1-RFP

Plasmid

#35401

Depositor

Robert Lefkowitz

Publication

Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

Insert

beta-arrestin-1 (Arrb1 Rat)

Tags

RFP

Expression

Mammalian

Promoter

T7

Available since

March 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article