We narrowed to 8,294 results for: construct

1. SAH0141-0.0-conP01

   Plasmid

   #79828

   Purpose

   Transcriptional interference assessment; strong interfering promoter, landing site for spacers

   Depositor

   Katja Arndt

   Article

   Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

   Inserts

   Blue fluorescent protein
   mCherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   bla P3 and conP01

   Available since

   Aug. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. SAH0146-0.5-conP01

   Plasmid

   #79831

   Purpose

   Transcriptional interference assessment; strong interfering promoter, 500 bp spacer

   Depositor

   Katja Arndt

   Article

   Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

   Inserts

   Blue fluorescent protein
   mCherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   bla P3 and conP01

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. SAH0098-scrPX

   Plasmid

   #79830

   Purpose

   Transcriptional interference assessment; no interfering promoter (scrambled)

   Depositor

   Katja Arndt

   Article

   Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95

   Inserts

   Blue fluorescent protein
   mCherry
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   bla P3 and scrPX (no promoter activity)

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDS3-aurJ

   Plasmid

   #70152

   Purpose

   Fusarium graminearum AurJ encoded on CEN/ARS yeast shuttle vector with DS3 selection marker

   Depositor

   Morten Sommer

   Article

   Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

   Insert

   aurJ
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD1

   Available since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pDS2

   Plasmid

   #70135

   Purpose

   CEN/ARS yeast shuttle vector with DS2 selection marker

   Depositor

   Morten Sommer

   Article

   Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

   Insert

   DS2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   AgTEF1

   Available since

   Dec. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNH052

   Plasmid

   #42158

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   YFP-RT-YFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNH054

   Plasmid

   #42160

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   mTFP1-RT-mTFP1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNH039

   Plasmid

   #42151

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   CFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNH058

   Plasmid

   #42161

   Depositor

   Colin Dolphin

   Article

   Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

   Insert

   TAP Tag::mTFP1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCGN R-Ras 38V, CVLS

    Plasmid

    #14735

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

    Insert

    R-ras (RRAS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    38V, CVLS

    Promoter

    Available since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pCGN R-Ras 38V, SVLL

    Plasmid

    #14736

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

    Insert

    R-ras (RRAS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    38V, SVLL

    Promoter

    Available since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pCGN R-Ras 38V, SPCVLL

    Plasmid

    #14737

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

    Insert

    R-ras (RRAS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    38V, SPCVLL

    Promoter

    Available since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

13. pCKTRBS-LmrR-BzdB1_nSP

    Plasmid

    #102926

    Purpose

    pCKTRBS derivative carrying LmrR-BzdB1_nSP chimera, also known as ChBz02

    Depositor

    George Church

    Article

    Juarez et al Nat Commun. 2018 Aug 6;9(1):3101. doi: 1

    Insert

    LmrR-BzdB1_nSP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PtetO

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

    Plasmid

    #185913

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

    Plasmid

    #185919

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

    Promoter

    CMV and T7

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRA1EGFP

    Plasmid

    #193791

    Purpose

    Expresses EGFP under the control of PR promoter, pUC origin of replication, Ampicillin selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PR promoter

    Available since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRK892

    Plasmid

    #71803

    Purpose

    Expression of mutated L. gasseri gusA from strong Ppgm promoter

    Depositor

    Rodolphe Barrangou, Todd Klaenhammer

    Article

    Duong et al Microb Biotechnol. 2011 May;4(3):357-67.

    Insert

    gusA3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D573A

    Promoter

    Ppgm

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAM1

    Plasmid

    #60487

    Purpose

    This plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genome

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al BMC Microbiol. 2011 Feb 22;11:38. doi: 1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBabe-Kras Wt

    Plasmid

    #75282

    Purpose

    Expresses human Kras wild-type

    Depositor

    Channing Der

    Insert

    KRAS (KRAS Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    None. Wild-Type

    Promoter

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV Puro

    Plasmid

    #68469

    Purpose

    Retroviral plasmid with multiple cloning site and puromycin resistance

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Puromycin Resistance
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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