159,352 results

1. pDSG288

   Plasmid

   #115607

   Purpose

   surface-displayed Ag3, used in composability experiments; low copy

   Depositor

   Ingmar Riedel-Kruse

   Article

   Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

   Insert

   regulated adhesin construct
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJMP12042

   Plasmid

   #222354

   Purpose

   Tn7 integrative vector with PabstBR promoter expression cassette and aprR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.

   Depositor

   Jason Peters

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TLCV2-APOBEC1-YTH

   Plasmid

   #178949

   Purpose

   Lentiviral vector for dox-inducible expression of APOBEC1-YTH in mammalian cells

   Depositor

   Kate Meyer

   Article

   Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

   Insert

   APOBEC1-YTH-T2A-GFP
   

   Use

   Lentiviral and Synthetic Biology; Inducible

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CAG-BFP-sesRNA-tTA2-WPRE-pA

   Plasmid

   #192070

   Purpose

   Expression of BFP, sesRNA in mammalian cells, with tTA2 as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for tdTom
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pXPR_011

   Plasmid

   #59702

   Purpose

   This lentiviral vector can be used to assay Cas9 activity.

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

   Insert

   EGFP sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETDuet S2

   Plasmid

   #171921

   Purpose

   Express RNA-based (p)ppGpp sensor and a NirFP

   Depositor

   Mingxu You

   Article

   Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

   Inserts

   RNA-based (p)ppGpp sensor
   NirFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGM202T7

   Plasmid

   #69993

   Purpose

   E. coli/Streptomyces expression vector containing the T7-promoter

   Depositor

   Günther Muth

   Article

   Muth et al Appl Microbiol Biotechnol. 2018 Nov;102(

   Inserts

   aphII
   tsr
   rep
   sso
   T7 promoter
   dso
   His-Orf
   

   Use

   Tags

   N-terminal and C-terminal His tag encoding sequen…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNos-PE2-attB

   Plasmid

   #149549

   Purpose

   Expression of PE2 enzyme under control of the germ cell specific nanos promoter. Can be used to generate transgenic flies with vermillion+ selection.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   PE2
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   nanos (nos)

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CROP_C9_Puro_FGFR3

   Plasmid

   #183291

   Purpose

   All-in-One CRISPRko system with a guide RNA that targets FGFR3 gene

   Depositor

   Prashant Mali

   Article

   Hu et al Biomaterials. 2022 Jan;280:121276. doi:

   Insert

   FGFR3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEGFP-C1 hSlp2-a

    Plasmid

    #40047

    Depositor

    Fernando Martin-Belmonte

    Article

    Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

    Insert

    hSlp2-a (SYTL2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 VDAC1-Linker-GFP

    Plasmid

    #211734

    Purpose

    Expresses GFP tagged VDAC1 in mammalian cells. GFP is attached to the C-terminus of VDAC1 with a flexible linker.

    Depositor

    Roland Malli

    Article

    Pilic et al bioRxiv 2023.09.08.556841

    Insert

    Voltage-dependent anion channel 1 (VDAC1 )
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRSII422

    Plasmid

    #35462

    Depositor

    Steven Haase

    Article

    Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epu

    Type

    Empty backbone

    Use

    Yeast 2 micron vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LcV2-Hygro

    Plasmid

    #91977

    Purpose

    LentiCRISPR v2 with Hygro cloned in place of Puro

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p7058 pHAGE-P-CMVt-N-HA-GAW-FDFT1

    Plasmid

    #100151

    Purpose

    Expresses FDFT1

    Depositor

    Peter Howley

    Article

    Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

    Insert

    FDFT1 (FDFT1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLIK-NFLAG-hEWSR1G511A-CMyc

    Plasmid

    #50734

    Purpose

    Produces lentivirus expressing human EWSR1G511A mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2G

    Depositor

    Zissimos Mourelatos

    Article

    Couthouis et al Hum Mol Genet. 2012 Jul 1;21(13):2899-91

    Insert

    EWSR1 G511A (EWSR1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG and Myc

    Expression

    Mutation

    Changed Gly 511 to Ala

    Promoter

    TRE

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pCAGGS_L_MARV

    Plasmid

    #135473

    Purpose

    Mammalian expression vector encoding Marburg virus L gene under control of the AG promoter

    Depositor

    Elke Mühlberger

    Article

    Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

    Insert

    L gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    silent mutation (C to T) at position 7861; insert…

    Promoter

    AG

    Available since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-hsRPA4 (MSW#517)

    Plasmid

    #208070

    Purpose

    Expression of GFP-tagged hsRPA4 in human cells

    Depositor

    Marc Wold

    Article

    Haring et al Nucleic Acids Res. 2010 Jan;38(3):846-58

    Insert

    RPA4 (RPA4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CMV-GFP-NMHC II-A

    Plasmid

    #11347

    Depositor

    Robert Adelstein

    Article

    Wei et al Mol Biol Cell. 2000 Oct . 11(10):3617-27

    Insert

    NMHC II-A (MYH9 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG

    Plasmid

    #155305

    Purpose

    Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.

    Depositor

    Stanley Qi

    Article

    Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

    Insert

    mCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
    

    Use

    Lentiviral

    Tags

    2xNLS-3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TFORF0500

    Plasmid

    #143709

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PPARGC1A (PPARGC1A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGL3 basic E2

    Plasmid

    #48747

    Purpose

    Expression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1)

    Depositor

    Joseph Takahashi

    Article

    Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

    Insert

    mPer2 promoter/enhancer region containing E-box2 (-112 to +98)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMS_366

    Plasmid

    #182131

    Purpose

    expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation

    Depositor

    George Church

    Article

    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118

    Inserts

    retron RNA
    ec.86 RT
    CspRecT
    

    Use

    Tags

    Expression

    Mutation

    incorporated rifampicin-resistance-conferring don…

    Promoter

    Available since

    Sept. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HaloTag-sec61b

    Plasmid

    #186959

    Purpose

    Express sec61b connected with N-terminus HaloTag

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    Sec61 (SEC61B Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJBL711

    Plasmid

    #140381

    Purpose

    Expresses OtrR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-OtrR-T
    

    Use

    Tags

    TEV-6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET51b-His-TEV-HaloTag7

    Plasmid

    #167266

    Purpose

    Overexpression of HaloTag7 in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    HaloTag7
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pIC291

    Plasmid

    #51403

    Purpose

    Retroviral expression of EGFP-TEV-S-tag SKA1 for protein localization and affinity purification (LAP)

    Depositor

    Iain Cheeseman

    Article

    Welburn et al Dev Cell. 2009 Mar;16(3):374-85. doi: 10

    Insert

    Ska1 IMAGE 3909951 (SKA1 Human)
    

    Use

    Retroviral

    Tags

    EGFP, S tag, and TEV protease site

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. LentiCRISPR v2-sgACSL4 clone2

    Plasmid

    #162129

    Purpose

    Lentiviral sgRNA plasmid targeting human ACSL4

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgACSL4 (ACSL4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. LentiCRISPR v2-sgACSL4 clone1

    Plasmid

    #162128

    Purpose

    Lentiviral sgRNA plasmid targeting human ACSL4

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgACSL4 (ACSL4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDONR223_BCL6_WT

    Plasmid

    #81869

    Purpose

    Gateway Donor vector containing BCL6 , part of the Target Accelerator Plasmid Collection.

    Depositor

    William Hahn, David Root, Jesse Boehm

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    BCL6 (BCL6 Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    R160C

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJBL713

    Plasmid

    #140383

    Purpose

    Expresses CtcS protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-CtcS-T
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge