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157,662 results

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pcDNA3-HA-VprBP

Plasmid

#133586

Purpose

Expresses human VprBP wildtype in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

Vpr (HIV-1) binding protein (DCAF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Wildtype human VprBP (Dcaf1)

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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EJ2GFP-puro

Plasmid

#44025

Depositor

Jeremy Stark

Article

Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

Insert

EJ2GFP egfp-based chromosomal break reporter

Use

Tags

Expression

Mammalian

Mutation

insertion of I-SceI, 3 frame stop, and flanking m…

Promoter

pCAGGS

Available since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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pT2AL200R150G-EGFP-LC3B-RFP-LC3BΔG

Plasmid

#168998

Purpose

Expresses GFP-LC3-RFP-LC3ΔG in zebrafish to measure autophagic flux

Depositor

Noboru Mizushima

Article

Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Unspecified

Tags

EGFP and mRFP1

Expression

Mutation

Promoter

Available since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-evoCjCas9-PEmax_dRnH-BlastR

Plasmid

#194063

Purpose

Mammalian expression of evoCjCas9 PEmax_deltaRnH

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-PEmax_dRnH

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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myc-KIF5C

Plasmid

#127618

Purpose

N-terminal myc-tagged mouse KIF5C

Depositor

Josef Kittler

Article

Twelvetrees et al Neuron. 2010 Jan 14;65(1):53-65. doi: 10

Insert

KIF5C (Kif5c Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-hPMCA2w/b

Plasmid

#47586

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2w/b (ATP2B2 Human)

Use

Tags

EGFP-HA-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Solo-mStayGold-CENPA

Plasmid

#227283

Purpose

Donor template for mStayGold insertion into the N-terminus of the CENPA locus. For centromere visualization. To be co-transfected with sgRNA plasmid px330-CENPA (Addgene #227282)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

CENPA Homology Arms flanking a mStayGold Tag (CENPA Human)

Use

CRISPR; Donor template

Tags

Expression

Mammalian

Mutation

Promoter

Promoterless

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-CENPA

Plasmid

#227282

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of CENPA (CENPA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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Rtn4a-GFP

Plasmid

#61807

Purpose

Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

Insert

Rtn4a (RTN4 Human)

Use

Tags

AcGFP

Expression

Mammalian

Mutation

Rtn4a Stop Codon removed

Promoter

CMV

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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Plxna1-Fc-His

Plasmid

#72122

Purpose

Expresses the extracellular region of the PlexinA1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Plxna1 (Plxna1 Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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PGC-1 alpha promoter 2kb luciferase

Plasmid

#8887

Depositor

Bruce Spiegelman

Article

Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

Insert

PGC-1 alpha promoter (Ppargc1a Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pLENTI_RPS3_Keima

Plasmid

#127140

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3_Keima (RPS3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hApg5

Plasmid

#22952

Depositor

Noboru Mizushima

Article

Mizushima et al J Biol Chem. 1998 Dec 18. 273(51):33889-

Insert

Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae) (ATG5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pTCF236 HLA-B*40:01

Plasmid

#180463

Purpose

E. coli expression of soluble MHC protein

Depositor

John Altman

Insert

HLA-B*40:01

Use

Tags

BSP41

Expression

Bacterial

Mutation

Replacement of transmembrane domain with enzymati…

Promoter

Available since

Feb. 23, 2022

Availability

Academic Institutions and Nonprofits only

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PM-B

Plasmid

#209876

Purpose

Dimerization dependent fluorescent protein B anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP B

Use

Tags

Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pFL

Plasmid

#110739

Purpose

Plasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP site

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Polyhedrin and p10 promoters

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pOTTC1328 - pAAV EF1a HA-hM3D(Gq)

Plasmid

#89149

Purpose

An AAV packaging plasmid that expresses HA-tagged hM3D(Gq) excitatory DREADD from the EF1a promoter.

Depositor

Brandon Harvey

Article

Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

Insert

hM3D(Gq)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA BfuAI stuffer

Plasmid

#50920

Purpose

U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLX208 CMV sTurboID (C)-HA-FRB-ERM

Plasmid

#153007

Purpose

Expresses split-TurboID C-terminal fragment targeted to endoplasmic reticulum membrane

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID (C) (G74)-ERM (Cb5)

Use

Lentiviral

Tags

FRB and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-mScarlet

Plasmid

#131000

Purpose

AAV vector to drive the expression of mScarlet under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Has Service

AAV8

Insert

mScarlet

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKIIa

Available since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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BirA pLEMO

Plasmid

#119818

Purpose

Express endogenous biotin ligase in E.coli

Depositor

Alexander Gabibov

Article

Lomakin et al Bioconjug Chem. 2019 Apr 25. doi: 10.102

Insert

Biotin ligase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Rhamnose promoter

Available since

April 30, 2019

Availability

Academic Institutions and Nonprofits only

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pGST-PSP-hTG

Plasmid

#85470

Purpose

bacterial expression of human tissue transglutaminase

Depositor

Jeffrey Keillor, Ray Truant

Article

Roy et al Protein Expr Purif. 2013 Jan;87(1):41-6.

Insert

hTG2 (TGM2 Human)

Use

Tags

GST and PSP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLITMUS*-PBBa_J23106-geneIII

Plasmid

#134352

Purpose

Phagemid

Depositor

Mark Isalan

Article

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

Gene III

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-2XNLS-RNaseH1 delta 1-27 (D210N)

Plasmid

#196703

Purpose

Plasmid for transient mammalian expression of catalytically inactive RNase H1 mutant.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

human RNaseH1 D210N

Use

Tags

Expression

Mammalian

Mutation

D210N, catalytically inactive

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD33-mf-lon

Plasmid

#21867

Depositor

Robert Sauer

Article

Gur et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

Insert

Lon (Mfl404 Mesoplasma florum)

Use

Tags

Expression

Bacterial

Mutation

all TGA codons changed to TGG codons

Promoter

Available since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

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pHCMV_VSAV_G

Plasmid

#207311

Purpose

Mammalian expression of Vesicular Stomatitis Arizona virus G protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

VSAV_G

Use

Tags

Expression

Mammalian

Mutation

mammalian codon optimization

Promoter

CMV promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pETDuet-1 Hepatitis C Virus NS3/NS4A

Plasmid

#61696

Purpose

inducible dual expression of NS3 protease along with its cofactor NS4A

Depositor

Michael Johnson

Article

Lee et al PLoS One. 2013 Oct 9;8(10):e75144. doi:

Inserts

NS4A
NS3

Use

Tags

his

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-LAMP2

Plasmid

#222321

Purpose

Synchronize the trafficking of LAMP2 from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAMP2 fused to SBP-EGFP (LAMP2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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p2xFlag CMV2-YAP2

Plasmid

#19045

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only

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pST39

Plasmid

#64009

Purpose

original polycistronic cloning vector for plasmid suite (generation I)

Depositor

Song Tan

Article

Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge