165,775 results

1. SpyCatcher003 S49C

   Plasmid

   #133448

   Purpose

   Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyCatcher003 S49C
   

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Contains S49C mutation

   Promoter

   T7

   Available Since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV[Exp]-EF1A>Tet3G:IRES:Neo

   Plasmid

   #198756

   Purpose

   Vector encodes a Tet-On Advanced transactivator under control of a constitutive EF1α promoter and confers resistance to the antibiotic G418

   Depositor

   Nael Nadif Kasri

   Article

   Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

   Insert

   Tet3G, IRES, Neo
   

   Use

   Lentiviral

   Promoter

   EF1A

   Available Since

   July 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDestTol2CG2-U6:gRNA

   Plasmid

   #63156

   Purpose

   Destination vector for Gateway based on vector #395 from the Tol2Kit, containing a gRNA scaffold under the control of a zebrafish U6 promoter, and cmlc2:GFP as a transgenesis marker.

   Depositor

   Leonard Zon

   Article

   Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

   Type

   Empty backbone

   Use

   CRISPR; Tol2 destination vector for gateway

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR223-ERBB2

   Plasmid

   #23888

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pHR_TetO_CD19-P2A-mCherry

   Plasmid

   #133803

   Purpose

   repressible expression of CD19 and mCherry in mammalian cells

   Depositor

   Wendell Lim

   Article

   Toda et al Science. 2018 Jul 13;361(6398):156-162.

   Insert

   putative ECD of H. sapiens CD19 (CD19 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9n(BB)-2A-Puro (PX462) V2.0

   Plasmid

   #62987

   Purpose

   Cas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9n-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Cas9 D10A nickase mutant

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEGFP-Rac1

   Plasmid

   #83950

   Purpose

   Rac1 FLIM donor for dynamic imaging of GTPase activity

   Depositor

   Ryohei Yasuda

   Article

   Hedrick et al Nature. 2016 Sep 28;538(7623):104-108. d

   Insert

   ras-related C3 botulinum toxin substrate 1 (RAC1 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657

   Plasmid

   #212963

   Purpose

   Lentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.

   Depositor

   Roland Rad

   Article

   Montero et al Nat Methods. 2024 Feb 26. doi: 10.1038/s

   Insert

   DR1_CasRx_GFP targeting spacer
   

   Use

   CRISPR and Lentiviral

   Tags

   TagRFP657

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-G-Flamp1

   Plasmid

   #188567

   Purpose

   green biosensor for cAMP in mammalian cells

   Depositor

   Jun Chu

   Article

   Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

   Insert

   G-Flamp1
   

   Tags

   His tag

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-mScarlet-I

    Plasmid

    #159172

    Purpose

    To express a bright monomeric red FP for labelling and detection of mammalian cells

    Depositor

    Pantelis Tsoulfas

    Insert

    mScarlet-I
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Mutation

    Codons were optimized to Human codon usage

    Promoter

    CMV

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. lentiSAM v2 (Puro)

    Plasmid

    #92062

    Purpose

    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Adam Karpf

    Article

    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 (sgRNA) and EF1a (dCas9-VP64)

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Integrin β8-GFP

    Plasmid

    #205093

    Purpose

    Express GFP-tagged human integrin β8 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 8 (ITGB8 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBAMD1-4

    Plasmid

    #61565

    Purpose

    Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

    Depositor

    Víctor de Lorenzo

    Article

    Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

    Type

    Empty backbone

    Use

    Synthetic Biology; Mini-tn5 vector

    Available Since

    Jan. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. RMCE K>R DNA-PKcs

    Plasmid

    #233917

    Purpose

    Low copy number provides expression of human DNA-PKcs with a single K>R substitution that ablates kinase activity.

    Depositor

    Katheryn Meek

    Article

    Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

    Insert

    human DNA-PKcs kinase inactive mutant K3753>R (PRKDC Human)
    

    Expression

    Mammalian

    Mutation

    K3753R

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pR26

    Plasmid

    #127372

    Purpose

    Allows for doxycycline-inducible gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

    Depositor

    Lance Miller

    Article

    Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

    Type

    Empty backbone

    Use

    CRISPR and Mouse Targeting

    Promoter

    tight TRE promoter

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC0056 - LwCas13a-msfGFP-NES

    Plasmid

    #105815

    Purpose

    Expresses active LwaCas13a-NES for mammalian RNA targeting

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LwCas13a
    

    Use

    CRISPR and Lentiviral

    Tags

    NES and msfGFP-NES-3xHA

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. iCas

    Plasmid

    #84232

    Purpose

    Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)

    Depositor

    Meng How Tan

    Article

    Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038

    Insert

    Cas9
    

    Tags

    2A-OFP co-expression and ERT2-ERT2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAX APP 751

    Plasmid

    #30138

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Expression

    Mammalian

    Mutation

    wild type splice variant 751

    Available Since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-Camk2-NES-Twitch-NR

    Plasmid

    #173043

    Purpose

    TnC-based calcium sensor with green-red FRET pair ratiometric readout

    Depositor

    Yiyang Gong

    Article

    Zhang et al Commun Biol. 2021 Jul 29;4(1):924. doi:

    Insert

    NES-Twitch-NR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CamK2

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAcGP67A-mMD2-proA

    Plasmid

    #187878

    Purpose

    baculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tag

    Depositor

    Bruce Beutler

    Article

    Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

    Insert

    MD2 (Ly96 Mouse)
    

    Expression

    Insect

    Mutation

    includes residues 19–160 of mouse MD-2

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-hSyn-DIO-mCherry

    Plasmid

    #50459

    Purpose

    Double floxed mCherry under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    mCherry
    

    Use

    AAV

    Tags

    N/A

    Promoter

    human Synapsin 1

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCRISPRi_Mxi1_yl_NHEJ

    Plasmid

    #91249

    Purpose

    CRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HR

    Depositor

    Ian Wheeldon

    Article

    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.

    Inserts

    Codon optimized dCas9-Mxi1
    KU70 sgRNA expression cassette
    KU80a sgRNA expression cassette
    KU80b sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Promoter

    SCR1'-tRNA and UAS1B8-TEF(136)

    Available Since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. DNA-PKcs PQR>ala

    Plasmid

    #83319

    Purpose

    human DNA-PKcs PQR>ala

    Depositor

    Katheryn Meek

    Article

    Cui et al Mol Cell Biol. 2005 Dec;25(24):10842-52.

    Insert

    human DNA-PKcs (PRKDC Human)
    

    Expression

    Mammalian

    Mutation

    5 phosphorylation sites in the PQR cluster substi…

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLL3.7

    Plasmid

    #11795

    Purpose

    3rd generation lentiviral vector that expresses shRNA under the mouse U6 promoter. A CMV-EGFP reporter cassette is included in the vector to monitor expression.

    Depositor

    Luk Parijs

    Article

    Rubinson et al Nat Genet. 2003 Mar . 33(3):401-6.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEMG-AIS

    Plasmid

    #225133

    Purpose

    For genome editing in P. putida KT2440, arabinose is used for the induction of I-sceI gene. Km.

    Depositor

    Lars Blank

    Article

    Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Oct. 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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26. mCherry-ZO1-C-14

    Plasmid

    #55165

    Purpose

    Localization: Tight Junctions, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    ZO1 (TJP1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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27. pSpCas9_T2A_iRFP670 sgAAVS1

    Plasmid

    #192477

    Purpose

    All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A iRFP670

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pGBKT7-GW

    Plasmid

    #61703

    Purpose

    Gateway compatible Y2H vector - BD

    Depositor

    Yuhai Cui

    Article

    Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mCardinal-N1

    Plasmid

    #54590

    Purpose

    Localization: N1 Cloning Vector, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Type

    Empty backbone

    Tags

    mCardinal

    Expression

    Mammalian

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pRPN265NB-enh-MNase

    Plasmid

    #166035

    Purpose

    Expresses a nanobody against GFP fused to micrococcal nuclease for purposes of green Cut & Run

    Depositor

    Marc Timmers

    Article

    Nizamuddin et al Nucleic Acids Res. 2021 May 21;49(9):e49

    Insert

    Glutathione-S-Transferase/enhancer/MNase
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    lac promoter / tac promoter

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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