We narrowed to 14,493 results for: NTS

Showing: 4681 - 4710 of 14493 results

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mTurquoise2-Rab7(WT)

Plasmid

#112959

Purpose

In vivo visualization of Rab7 (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Rab7

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCR4 hNFATc1-EE

Plasmid

#17857

Depositor

Jerry Crabtree

Article

Northrop et al Nature. 1994 Jun 9. 369(6480):497-502.

Insert

NFATc1 (NFATC1 Human)

Tags

EE

Expression

Bacterial

Available Since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
p2T-CMV-BE4maxCP1028-BlastR

Plasmid

#152993

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

BE4-nSpCas9 CP1028-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pMOD_A0103

Plasmid

#91000

Purpose

Module A, Promoter: CmYLCV, Gene: AtCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9

Use

CRISPR

Promoter

CmYLCV

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMSR

Plasmid

#161983

Purpose

Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile 630. Uses inducible expression of CD2517.1 toxin as a counter-selection marker

Depositor

Olga Soutourina

Article

Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac_human-IL-18

Plasmid

#214338

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMOD_A1511

Plasmid

#91037

Purpose

Module A, Promoter: OsAct1, Gene: Csy4-P2A-TaCas9, Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Csy4-P2A-TaCas9

Use

CRISPR

Promoter

OsAct1

Available Since

July 10, 2017

Availability

Academic Institutions and Nonprofits only
pJBL711

Plasmid

#140381

Purpose

Expresses OtrR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-OtrR-T

Tags

TEV-6XHis

Expression

Bacterial

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:(MCS)-S11-DI-GFP1–9

Plasmid

#108260

Purpose

Binary vectors used for the inducible expression of dual intein-coupled tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pET-DUET-1-mRhubarb713-HO

Plasmid

#141199

Purpose

Expresses mRhubarb713 fluorescent protein and heme oxygenase

Depositor

Elad Firnberg

Article

Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

Inserts

mRhubarb713
heme oxygenase

Tags

6x-His

Expression

Bacterial

Mutation

R134H, W309R, Q310A compared to iRFP

Promoter

T7

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDR111_mannose_Bgal no NLS

Plasmid

#188399

Purpose

Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Tags

PhoD secretion peptide

Expression

Bacterial

Promoter

D-mannose

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJIT163-2NLSCas9

Plasmid

#53064

Purpose

Expression of rice codon-optimized Cas9 in plant cells

Depositor

Caixia Gao

Article

Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

Insert

Cas9

Use

Plant expression

Mutation

Rice codon optimized

Promoter

2x35S

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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DCIP

Plasmid

#193860

Purpose

Encodes SV40-mCherry-GFP1-10 in pDGB1-aR backbone for plant expression. Requires pSOUP for replication in agrobacterium.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

NLS-mCherry-GFP1-10

Tags

SV40 NLS

Expression

Plant

Promoter

35S

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-GFP-mCherry-3pGW

Plasmid

#53965

Purpose

Bidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherry

Depositor

Saeed Tavazoie

Article

Oikonomou et al Cell Rep. 2014 Apr 10;7(1):281-92. doi:

Inserts

GFP
mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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Erk2

Plasmid

#29582

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Erk2 (Mapk1 Rat)

Tags

HIS6 and TEV

Expression

Bacterial

Mutation

Starting Met removed from Erk2

Available Since

July 16, 2011

Availability

Academic Institutions and Nonprofits only

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ITPKA-mScarlet-I

Plasmid

#98829

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

ITPKA

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pRU319

Plasmid

#167686

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::spCas9

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCMU-LEr

Plasmid

#61185

Purpose

Fluorescent reporter for late endosome mCherry-MtRAB5A2 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB5A2

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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His6-TEV-Venus-pBluescript

Plasmid

#166756

Purpose

For purification of Venus fluorophore

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Venus

Tags

6x Histidine tag, and TEV protease site

Expression

Bacterial

Promoter

T7

Available Since

March 26, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE655

Plasmid

#153253

Purpose

M1 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pKin1B

Plasmid

#31604

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

kinesin-1B (Kif5b Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pBac{3XP3::DsRed, Pactin::Ptrsps}

Plasmid

#86862

Purpose

Expresses DsRed in eyes and ubiquitous expression of P-transposase

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Insert

pACNNTNPII-S129A

Expression

Insect

Mutation

S129A

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-CDAmax-BlastR

Plasmid

#152995

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

CDA-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA

Plasmid

#64973

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

T7 promoter

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pKM126

Plasmid

#105134

Purpose

Clostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriT

Depositor

Joseph Sorg

Article

McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

Inserts

Upstream & Downstream pyrE deletion region
tetR
Cas9
gRNA

Use

E. coli - c. difficile shuttle vector

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5_tAKARβ

Plasmid

#119914

Purpose

Mammalian expression of the tAKARβ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKARβ

Expression

Mammalian

Available Since

Feb. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BYG

Plasmid

#158126

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (wild-type construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

mTagBFP

Expression

Yeast

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pUb RxCas13d + RxCas13d guide RNA

Plasmid

#176303

Purpose

Expresses RxCas13d and its associated guide RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

Insert

RxCas13d

Tags

HA tag

Expression

Insect

Promoter

Ubi-p63e

Available Since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAT15068-evoCBE

Plasmid

#163013

Purpose

plasmid expressing evoCBE

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

evoCBE

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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PMXS-SLC2A3

Plasmid

#72877

Purpose

expresses SLC2A3 in mammalian cells

Depositor

David Sabatini

Article

Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

Insert

SLC2A3 (SLC2A3 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only