We narrowed to 88,565 results for: Mal;

1. EF1a_SPIB_P2A_Hygro_Barcode

   Plasmid

   #120483

   Purpose

   Barcoded lentiviral vector to express SPIB in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   SPIB (SPIB Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV+ episome

   Plasmid

   #169742

   Purpose

   CMV+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   pCMV-tetO2

   Available Since

   Oct. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. WT (AF20)

   Plasmid

   #21359

   Depositor

   Gregory Germino

   Article

   Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

   Insert

   autosomal dominant polycystic kidney disease type I (PKD1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   wild-type sequence*

   Available Since

   Jan. 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX-FKBP-Rab5-Blasticidin

   Plasmid

   #120715

   Purpose

   Expresses early endosome-targeted recruiter protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   Rab5a
   

   Use

   Lentiviral

   Tags

   FKBP and mCherry

   Expression

   Mammalian

   Mutation

   Amino acids 2-215

   Promoter

   CMV

   Available Since

   Jan. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-FLAG-CaMKIID-K43M

   Plasmid

   #186830

   Purpose

   Expresses FLAG-CaMKIID-K43M in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   Camk2d (Camk2d Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed Lysine 43 to Methionine

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX-FKBP-Rab11-Blasticidin

   Plasmid

   #120717

   Purpose

   Expresses recycling endosome-targeted recruiter protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   Rab11a
   

   Use

   Lentiviral

   Tags

   FKBP and mCherry

   Expression

   Mammalian

   Mutation

   Amino acids 2-216

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-Chronos-mT-Sapphire-NRN

   Plasmid

   #149692

   Purpose

   For packaging and expression of Chronos-mT-Sapphire featuring a neuritin (NRN) 3' UTR in neurons via AAV

   Depositor

   Robert Malenka

   Article

   Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

   Insert

   Chronos
   

   Use

   AAV

   Tags

   mT-Sapphire

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ef1a_LIN28_Ires_Puro

   Plasmid

   #18921

   Depositor

   Linzhao Cheng

   Article

   Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

   Insert

   LIN28 (LIN28A Human)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 4, 2008

   Availability

   Academic Institutions and Nonprofits only

9. Tmem214 pmRFP-N2 (1348)

   Plasmid

   #62047

   Purpose

   mammalian expression of nuclear envelope transmembrane protein

   Depositor

   Eric Schirmer

   Article

   Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

   Insert

   Tmem214 (TMEM214 Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pEGIP*35

    Plasmid

    #26776

    Purpose

    Enables the creation of a chromosomally integrated mutant EGFP target sequence by lentiviral transduction to assess the efficiency of HR-mediated gene targeting

    Depositor

    Linzhao Cheng

    Article

    Zou et al Cell Stem Cell. 2009 Jul 2. 5(1):97-110.

    Insert

    GFP*35
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tmem214 pEGFP-N2 (1072)

    Plasmid

    #62026

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    Tmem214 (TMEM214 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

12. AsCas12a-DR-cr in PBCSK-

    Plasmid

    #126640

    Purpose

    Cloning vector AsCas12a DR-crRNA for expression in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

    Insert

    AsCas12a Full Length Direct Repeat crRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6 Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNoTag-DGCR8

    Plasmid

    #164694

    Purpose

    Express DGCR8 without tag in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Insert

    DGCR8 (DGCR8 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-ChrimsonR-LSSmOrange-NRN

    Plasmid

    #149693

    Purpose

    For packaging and expression of ChrimsonR-LSSmOrange featuring a neuritin (NRN) 3' UTR in neurons via AAV

    Depositor

    Robert Malenka

    Article

    Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

    Insert

    ChrimsonR
    

    Use

    AAV

    Tags

    LSSmOrange

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-OTUB1 T134R-pcDNA3.1

    Plasmid

    #118211

    Purpose

    Expresses FLAG-OTUB1 T134R mutant in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    OTUB1 (OTUB1 Human)
    

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    changed threonine 134 to arginine

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-CibN-dCas9

    Plasmid

    #60551

    Purpose

    Expresses CibN-dCas9 in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    CibN-dCas9
    

    Use

    CRISPR

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    inactivating Cas9 mutations D10A and H840A

    Promoter

    CMV

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV_FLAG-NFL(WT)

    Plasmid

    #182659

    Purpose

    Expresses wild-type mouse neurofilament light chain with an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Insert

    wild type mouse neurofilament light chain (Nefl Mouse)
    

    Tags

    FLAG tag (DYKDDDDK)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-CreiLOV-P2A-FusionRed

    Plasmid

    #192818

    Purpose

    Expresses CreiLOV with FusionRed tag in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

    Insert

    CreiLOV
    

    Use

    AAV

    Tags

    FusionRed

    Expression

    Mammalian

    Promoter

    chicken β-actin promoter

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTS105 pHAGE2 CMVtetO2 MCS-TEV-22xGS-GFP

    Plasmid

    #199358

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TEV-EGFP

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNAIIIB cdc42 WT

    Plasmid

    #61635

    Purpose

    mammalian expression of cdc42 wt

    Depositor

    JS Gutkind

    Article

    Coso et al Cell. 1995 Jun 30;81(7):1137-46.

    Insert

    cdc42 (CDC42 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only