We narrowed to 7,533 results for: ski

1. HA-MBP-TEVpCS-ADGRG7 CTFdeltaTA-FLAG

   Plasmid

   #217738

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRG7 (ADGRG7 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-421

   Available Since

   April 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDF0234 pCMV - huDisCas7-11 dead mutant

   Plasmid

   #172509

   Purpose

   Encodes human codon optimized catalytically dead DisCas7-11 protein alone for expression in mammalian cells

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   pCMV - huDisCas7-11 dead mutant
   

   Expression

   Mammalian

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-N1 Raptor Halo

   Plasmid

   #112754

   Purpose

   express Raptor-Halo

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Raptor-Halo (RPTOR Human)
   

   Tags

   Halo

   Expression

   Mammalian

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA5/FRT/TO_H2B_HaloTag9_T2A_EGFP

   Plasmid

   #169332

   Purpose

   Nuclear expression of HaloTag9 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag9 (H2BC21 Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hysn-flex-dsRed-shscramble

   Plasmid

   #71383

   Purpose

   Expresses scramble shRNA Cre-dependently by flex switch

   Depositor

   William Wisden

   Article

   Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

   Insert

   human synapsin promoter-flex switch-dsRed-shscramble
   

   Use

   AAV, Cre/Lox, and RNAi

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. IMS-HyPer2

   Plasmid

   #60248

   Purpose

   Genetically-encoded fluorescent hydrogen peroxide indicator HyPer2 targeted to mitochondrial intermembrane spase.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

   Insert

   IMS-HyPer2
   

   Tags

   Mitochondrial intermembrane space targeting signa…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCXLE-mCherry

   Plasmid

   #193293

   Purpose

   episomal vector expressing mCherry fluorescent protein

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   mCherry
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO_Vim-SNAP-tag2

   Plasmid

   #226518

   Purpose

   Mamalian cell expression of intermediate filaments (vimentin) fused to SNAP-tag2

   Depositor

   Kai Johnsson

   Article

   Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

   Insert

   Vimentin-SNAP-tag2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2 GFP-GSK3-MAPK

   Plasmid

   #29689

   Purpose

   GFP reporter protein fused to three GSK3 phosphorylation sites

   Depositor

   Edward De Robertis

   Article

   Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

   Insert

   GFP glycogen synthase kinase 3 recognition site primed by MAPK
   

   Tags

   Flag and Flag-EGFP

   Expression

   Mammalian

   Mutation

   recognition sites only, and E3 ligase site

   Promoter

   SP6

   Available Since

   July 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-eLACCO1.1

    Plasmid

    #168788

    Purpose

    Neuronal expression of fluorescent sensor for extracellular lactate

    Depositor

    Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    eLACCO1.1
    

    Use

    AAV

    Tags

    ER export and GPI

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-U6-gRNA scaffold-UbC-mCherry-PuroR

    Plasmid

    #232183

    Purpose

    Lentiviral plasmid for cloning an spCas9 gRNA with puromycin resistance and mCherry

    Depositor

    Charles Gersbach

    Article

    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6, hUbC

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-2Aneo v2

    Plasmid

    #80033

    Purpose

    Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

    Depositor

    Hiroyuki Konishi

    Article

    Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

    Type

    Empty backbone

    Use

    AAV and Cre/Lox; A platform vector to construct t…

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. phMYT1L-N106

    Plasmid

    #60861

    Purpose

    Expresses MYT1L under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    MYT1L (MYT1L Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. piGECI-N1

    Plasmid

    #160421

    Purpose

    Cytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECI

    Depositor

    Vladislav Verkhusha

    Article

    Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

    Insert

    iGECI
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. FRB-BFP-GCN4-ppKin14

    Plasmid

    #240426

    Purpose

    -end oriented kinesin for rapalog induced pulling experiments

    Depositor

    Anna Akhmanova

    Article

    Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755

    Insert

    ppKin14Vib
    

    Tags

    FRB, GCN4, and tagBFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-850

    Available Since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-fLuc=tdT

    Plasmid

    #120870

    Purpose

    Piggybac transposon plasmid for live animal optical imaging

    Depositor

    Jordan Green

    Article

    Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

    Insert

    pcDNA3-fLuc=tdT
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-GFAP-eLACCO1.1

    Plasmid

    #168790

    Purpose

    Astrocyte expression of fluorescent sensor for extracellular lactate

    Depositor

    Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    eLacco1.1
    

    Use

    AAV

    Tags

    ER export and GPI

    Expression

    Mammalian

    Promoter

    GFAP

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CAG-eLACCO1.1

    Plasmid

    #168789

    Purpose

    Fluorescent sensor for extracellular lactate

    Depositor

    Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2021 Dec 6;12(1):7058. doi:

    Insert

    eLACCO1.1
    

    Use

    AAV

    Tags

    ER export and GPI

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. 5'-BirA-FLAG GFP minimal CMV pCDNA5

    Plasmid

    #212087

    Purpose

    LR vector for integration of GFP into N2a FRT rtTA3 expression cells (BioID vector)

    Depositor

    Benjamin Blencowe

    Article

    Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

    Insert

    GFP
    

    Expression

    Mammalian

    Available Since

    Jan. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET30a(+)-PE2

    Plasmid

    #180015

    Purpose

    Expressing PE2 protein in E.coli for protein purification

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    PE2
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    Lac operator-controlled T7 promoter

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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