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Plasmid#89334PurposeElk1 cDNA encoding aa 307-428, aa383 mutated from ser-Ala, The PCR product was cut with BamH1 and Xba 1(365bp). pSG424 was cut with Bam H1 and Xba1. The Elk1 fragment was ligated into the BamH1DepositorInsertELK1 (ELK1 Human)
UseTagsGal 4ExpressionMammalianMutationencodes aa 307-428, changed serine 383 to alanine…PromoterAvailable sinceApril 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
GAL-p300-1901-2414
Plasmid#89103Purposemammalian expression of human p300 amino acids 1901-2414 with N-terminal GAL4 DNA binding domainDepositorInsertp300 (EP300 Human)
UseTagsGAL4 DBDExpressionMammalianMutationaa 1901-2414PromoterAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQCXIB-FLAG-hDIXDC1-L-WT
Plasmid#61224PurposeRetrovirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseRetroviralTagsFLAGExpressionMutationPromoterAvailable sinceMarch 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-TER+-shDIXDC1-Hu-16
Plasmid#61237PurposeEntry clone encoding shRNA to human DIXDC1DepositorInsertDIXDC1 (DIXDC1 Human)
UseEntr cloneTagsExpressionMutationPromoterAvailable sinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Blast-FLAG-hDIXDC1 WT
Plasmid#61220PurposeLentivirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterPGKAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Hygro-FLAG-hDIXDC1 WT
Plasmid#61213PurposeRetrovirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseRetroviralTagsFLAGExpressionMutationPromoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV/TO-Puro-FLAG-hDIXDC1 WT
Plasmid#61218PurposeLentivirus expressing long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseLentiviralTagsFLAGExpressionMutationPromoterCMV/TOAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-MYC-hDIXDC1-L-F630S
Plasmid#61241PurposeEntry clone encoding long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseEntr cloneTagsMYCExpressionMutationChanged Phenylalanine 630 to SerinePromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-MYC-hDIXDC1-L-S592A
Plasmid#61240PurposeEntry clone encoding long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseEntr cloneTagsMYCExpressionMutationChanged Serine 592 to AlaninePromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-MYC-hDIXDC1-L-WT
Plasmid#61239PurposeEntry clone encoding long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseEntr cloneTagsMYCExpressionMutationPromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-FLAG-hDIXDC1-L-S592A
Plasmid#61211PurposeEntry clone encoding long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseEntr cloneTagsFLAGExpressionMutationChanged Serine 592 to AlaninePromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-FLAG-hDIXDC1-L-F630S
Plasmid#61212PurposeEntry clone encoding long isoform of human DIXDC1DepositorInserthuman DIXDC1 (DIXDC1 Human)
UseEntr cloneTagsFLAGExpressionMutationChanged Phenylalanine 630 to SerinePromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-10-3xFLAG-LGP2-MI
Plasmid#58682PurposeExpresses LGP2 with a mutation to motif I in mammalian cellsDepositorInsertLGP2-MI (DHX58 Human)
UseTags3x FLAGExpressionMammalianMutationLysine 30 mutated to AlaninePromoterCMVAvailable sinceJuly 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
CS2+MYC-Etv5:T139D
Plasmid#49097Purposeexpression of Etv5 T139D mutantDepositorInsertEtv5 T139D (etv5a Zebrafish)
UseXenopus/avian/zebrafishTagsMYCExpressionMammalianMutationT139DPromoterCMVAvailable sinceNov. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cs2+Etv5 S142D
Plasmid#49096Purposeexpression of Etv5 S142D mutantDepositorInsertEtv5 S142 D (etv5a Zebrafish)
UseXenopus/avian/zebrafishTagsExpressionMammalianMutationS142DPromoterCMVAvailable sinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
TRS6E1b-luc(-732/-721) mutant 3
Plasmid#45386DepositorInsert6 copies of hPAI-1 promoter -732/-721 mutated at -723/-721
UseLuciferaseTagsluciferaseExpressionMammalianMutation-723/-721 mutated from tgt to acaPromoterAvailable sinceJune 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
TRS6E1b-luc(-732/-721) mutant 2
Plasmid#45385DepositorInsert6 copies of hPAI-1 promoter -732/-721 mutated at -730/-728
UseLuciferaseTagsluciferaseExpressionMammalianMutationmutated at -730/-728 from aca to tgtPromoterAvailable sinceJune 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP G protein Gamma 3(F70A,F71A) in pcDNA3.1
Plasmid#42185DepositorInsertG protein Gamma 3(F70A, F71A) (Gng3 Mouse)
UseTagsEGFPExpressionMammalianMutationF70A, F71APromotercmvAvailable sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP G protein Gamma 3(K68A,K69A) in pcDNA3.1
Plasmid#42188DepositorInsertGamma 3(K68A,K69A) (Gng3 Mouse)
UseTagsEGFPExpressionMammalianMutationK68A,K69APromotercmvAvailable sinceMarch 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
XE159 GFP-delta LZ-XDpr-delta PDZB-CS2+
Plasmid#16763DepositorInsertdelta LZ-XDpr-delta PDZB (dact1-b Frog)
UseXenopus expressionTagsgfpExpressionMutationXDpr that is doubly truncated to delete the first…PromoterAvailable sinceMarch 13, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHT (His10-4xFRB-SNAP-Lck G2A)
Plasmid#177887PurposeBaculo expression of decahistidine tag fused to 4xFRB, 1xSNAP tag and human Lck G2ADepositorInsertLck (LCK Human)
UseTags10xHis-TEV-4xFRB-SNAP tagExpressionInsectMutationG2APromoterPolyhedrinAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
EphA2-YFP
Plasmid#108852PurposeEncodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linkerDepositorInsertEPHA2 (EPHA2 Human)
UseTagsLabeled with eYFP on the C-Terminus via a 15 amin…ExpressionMammalianMutationPromoterAvailable sinceApril 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-EGFP-NLS-P2A-mCherry-PTS1
Plasmid#87803PurposeMammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using P2A. NLS and PTS1 can be replaced by proteins of interest.DepositorInsertsNLS
PTS1
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterCMV promoter, tetracycline operatorAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPGK-T7/2-CD44v3 (fl)
Plasmid#137814Purposeexpression of CD44 proteins in mammalian cellsDepositorInsertCD44v3 (fl) (CD44 Human)
UseTagsGFPExpressionMammalianMutationfull length and N280D in the V3 regionPromoterPGKAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-Necl5-ScNeo
Plasmid#209901PurposeTo monitor the status of Necl-5, the plasmid encodes a recombinant Necl-5 fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertNecl5-ScNeo (Pvr Mouse)
UseLentiviralTagsmNeonGreen and mScarletExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIpuro-TGFa-ScNeo
Plasmid#209895PurposeTo monitor the status of TGFα, the plasmid encodes a recombinant TGFα fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent proteinDepositorInsertTGFa-ScNeo (TGFA Human)
UseLentiviralTagsGGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…ExpressionMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
EGFP-CAAX
Plasmid#86056PurposeA mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.DepositorInsertHomo sapiens Harvey rat sarcoma (HRAS Human)
UseTagsGFPExpressionMammalianMutationcontains the region from 825-857 of HRAS as a far…PromoterCMVAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.3 LRP5
Plasmid#115907PurposeExpresses human LRP5 in mammalian cells.DepositorInsertLRP5 (LRP5 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV14-GINIP
Plasmid#204845PurposeExpress 3x FLAG tagged-GINIP in mammalian cellsDepositorInsertPHF24 (KIAA1045 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3 2 kb prom. CD274
Plasmid#107003PurposeModified Luciferase expression vectorDepositorInsertCD274 (CD274 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only