We narrowed to 22,334 results for: CRISPR

1. pCXLE-dCas9VPH-T2A-GFP-shP53

   Plasmid

   #102895

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-p65-HSF1 followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9VPH-T2A-GFP-shP53
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)

   Plasmid

   #164040

   Purpose

   Expression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2

   Depositor

   Baohui Chen

   Article

   Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

   Insert

   sgTS5-sgTS6-sgTS7
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   mouse U6

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. NAE1 G7.3 gRNA

   Plasmid

   #90776

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NAE1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   NAE1 (Guide Designation G7.3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ133A2.0

   Plasmid

   #99891

   Purpose

   Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-EEA-g1-PGK-Puro

   Plasmid

   #102908

   Purpose

   PiggyBac transposon system construct for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   EEA-guide1-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-Cas12i-Max-T2A-EGFP

   Plasmid

   #188268

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Chen et al Innovation (Camb). 2022 May 26;3(4):1002

   Insert

   Cas12i-Max
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Cas12i-HiFi-T2A-EGFP

   Plasmid

   #188269

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Chen et al Innovation (Camb). 2022 May 26;3(4):1002

   Insert

   Cas12i-HiFi
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. RFN_EGFP_81

   Plasmid

   #60073

   Purpose

   Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   pair of multiplex gRNAs targeting EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pTU387

   Plasmid

   #178981

   Purpose

   pACYC Duet-1 containing full Type III-E system Candidatus “Scalindua brodae” CRISPR array

   Depositor

   Stan Brouns

   Article

   van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

   Insert

   CRISPR array from Candidatus Scalindua brodae
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GG-EBNA-EEA-guide1-PGK-Puro

    Plasmid

    #102904

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of a gRNA targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-guide1-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pYPQ133C2.0

    Plasmid

    #99893

    Purpose

    Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsU6

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSP2283

    Plasmid

    #70702

    Purpose

    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

    Inserts

    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX458_TFE3_2

    Plasmid

    #86326

    Purpose

    Encodes gRNA for 3' target of human TFE3

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against TFE3 (TFE3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. A3B-Cas9n-UGI-NLS

    Plasmid

    #198889

    Purpose

    Express full-length A3B BE3-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ131C2.0

    Plasmid

    #99886

    Purpose

    Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsU6

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYPQ132C2.0

    Plasmid

    #99889

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsU6

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pV1539

    Plasmid

    #111434

    Purpose

    Genedrive entry vector for Candida albicans

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCSN061

    Plasmid

    #101725

    Purpose

    Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Inserts

    S. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
    KanMX marker expression cassette.
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Heterologous TEF1 promoter from A. gossypii. and …

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. PLK1 G5.1 gRNA

    Plasmid

    #90834

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PLK1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    PLK1 (Guide Designation G5.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. psgRNA2.02-PP7-ccdB

    Plasmid

    #99912

    Purpose

    Basic cloning vector for the expression of PP7 inserted sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge